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CRIMIC CR01162

Gene lqfR

CG42250

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:22412304..22412325	94A12	FBti0202700	81200

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CGTTCGAAACGTGATGGAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACACTGGACACGACAGCTC	TGGGCCAATTGTCGCAAATG	TCTATCAACGTCGCAGCCAA	GACTGGGTGAGTCGCTATCG

 

Amplicon 5'

Amplicon 3'

AACACTGGACACGACAGCTCACCAAATATTAGCTGCTGTGATTTTGTTTTACAATTTTCGTTTAAATTTTTACATTTTTGTTTGCGGAAAGCGCGGGTGCGAGACAGGTGCGCCGTTTCCATGCAAAAGTGCAAATCGTCAGCTGCAAAACAGTCAGAAAACGGCACGCCAAGCGAAAACGAGTTCGACGTCTTGTTGTAGTTGGATTTTCCAGCCACTTTTCGACGAGATTGTGTGAAAAATTCCGTAGCATTCAATAGTCTCAAAATGGTGGATAAATTCATCAGCATGTGGAAAGTGCGCGAATTGGCGGACAAGGTGTAAGTGATCAGGAATATATAGGATATATCCATACGTATATGTAATGGGATGTGTGTATAACATTCCCTTAACGCCGAAGTGAATGTTAAGACTCCCACGCCCCAAGTTTTCTGATTTCCCGCCACGCCCCCAACTCCTCAAGGATTTCAAAAGCCCTATGTCATGCATAAATCCCTAATAATTCATAAAGATATCTTACTTATGGATCAACTGCATCGGGGTGCTGGATTAGGTTCACGTTTTTAACTGCTTTCAAGGTTGTTCGCCATTTCAAGAGAGCTTTACCAACACAGCCACATTCCGATATTGATATCCATGTGTATATGCGCCTGTGTGTGTGGGGCTTTATCAAGAAACAATTTGTTTGCTTTCGACTGCCCAAATATAACAATTTTTTCACCTGTGTTAACAGCTCCACTTCCAGGCTTTCCAGTGAAATGGGTGTTTCTCTTTCCCCCATCGAATTATTAATTATGGGTGTCCCCTTTCACCTGCGCCTTTTATGGGGTAGTTATTTCTAAAAGCGAGCCTATAGTTCGAATAAATAATGGTGGCTCGATAGCCAACACCGAACTCATTAGGTTAAGTGCGCGGGTACACCTTTCCTAGGGCTTATCTCGAACTGATAAGGAACACCTAGATGGCCCATTTGCGACAATTGGCCCA

TCTATCAACGTCGCAGCCAAGCCATGCGTATTACTTATTTATCTGCATCTTTGCATTGTATATCCATATCGTCACACAACGTTTACTTAAAAATACCAGTTCTGATTAACTGGCCCTCTATCTATTTGATTATCTATTCAATTGAGTTTACGGAATGTCCCAGTCAATATGCCTTTTTTCTTTACTTAAGTATATATCAATTATTTAAGGAAACAAGCTCGGATTAAGATTTGTATTTACATATGTACAAAAGTTCTACGACTAACATCGTGAATAGTTAATAGTTACTTTAAAATAAAACAATGACTTCGTTCCCACTTGCAGCACCAATGTCGTGATGAATTACACGGAAACGGAGGGCAAGGTGCGGGAGGCCACCAACGATGATCCTTGGGGGCCTACAGGACCCCTCATGCAGGAATTGGCCTATTCCACCTTCTCATACGAAACATTCCCGGAGGTGATGTCCATGCTGTGGAAGCGCATGCTGCAGGACAATAAAACCAACTGGCGACGCACGTACAAGGTAACGTTTTCAGTTTAAACTAATAAATATAGAGCTAACGAGTATATATTTACTTACAGAGCCTCCTTCTGCTAAACTATTTGGTGCGAAACGGCTCTGAACGGGTGGTAACCTCCTCTCGGGAGCACATCTACGATCTGCGCTCGCTGGAGAACTATACATTCACCGACGAGGGCGGCAAGGATCAGGGTATTAATGTTAGGCATAAGGTACGAGAGCTTATAGACTTTATTCAGGATGATGATCGTTTGCGCGAGGAGCGCAAAAAGGCGAAGAAGAACAAGGACAAGTACATCGGCATGAGCAGCGACGCCATGGGCATGCGAAGCGGTGGCTACAGCGGCTATAGCGGTGGATCTGGAGGAGGCGGCGGTGGCAGCGGTGGCTACAATGATGGCGACTATCGCTCTAGTCGTGGAGACAATTGGTGTAAGTAAGAAATTTTTATTTTTTTAAGATTTTTGTTCAAATTCTCTATATGTAATATTAATATTGATGATTTTTCTTCAGACTCCGACAAAAGCGCCGACAAGGATCGGTATGAGGATGATGATACTCACTACGATGGAGAGCGAGAGGGATCCGATAGCGACTCACCCAGTC

 

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