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CRIMIC CR01163

Gene CG43689

CG43689

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:4080144..4080171	4A4	FBti0210324	86337

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCAGCAACTGAGTACCGCCTTCGAT	CAACCCGACAAACTGTCAAGTTCCCA

Images

Construct Design Sequences

sgRNA sequence	GACGATGAGGATGTATGTAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACAGAACTGTTTTTCGGCGC	GTCGTCGCCTGTGGAAATTG	GGGTAAGTGGGTGTGGCATG	CATGACAGTCAGCCAGAGGG

 

Amplicon 5'

Amplicon 3'

ACAGAACTGTTTTTCGGCGCGATAAGTGCTTTGCACAGATTGTGTGAACAAAGATTTGAGCAGTGCTGTAAATAGCACTACGATTCAAACATAAAATAGTGCTGTAAATGGTACTACGACCACAAACAGTTCTGTTAACAGCAATGTAAACTTGGTTTCAATAATTTTGAAAGATGCAGTACAGTAGTTCACCTGCGATTCGTTGTGGTTCGGCTGGGTTTCCGTTTCCGGTTCGATTGAACGATCGGTTGATGTGCGAGACTTCTTGTTGCATTGAGTTTACCGCTTCAATTGGCAAAATCTGAGTAAAGCCCCTTGTCCCTACCGCCCCCCCACCAACCAACCCACTAACCAAGCACTCCCTTAAGAGAGACAGAGACGGACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGATAGAGTGTATGTGTGAGTCTTTTGATGTGTGTCTGATCATTGAACGAAAGAAAATAAGAAAAACTAGCGAGAGACACTCAATTTCCCTCGGTAATAGGCAACATTATGACGATTATTATACCGCTGGTTTTTGTTGTCATTATTATTTGCACAGACTCCTGCCCGTGACTCCTGCCCCACATATCTAAAAATAAAACCCTTTTTGCAGCTCATTCAGCAGCCAAAAAGACATTAAGCGATTTTGTCGTTATGAAAATGGGATCGGTAAGGGGGTGTGTGCTGTACAATTCGGTGTAGTTGGGGGTTTCGAGCGCACAGCAACTAATGGCTTGTAAACCCTTTATGCCACACAACACAGAGGCACATGGACAGACATCTTTTGGTTGAGTGCCTGAGCCTTGAGCTTTGCCTTCTACTAATTTCGCAGTACAATTTCCACAGGCGACGAC

GGGTAAGTGGGTGTGGCATGATTAGATATAAAGCATTATGAGCACGTTTTCCTTCAAAAACCACTTTTGCATATTTATTTTGTCATTATGTTTTGGCATTAATATGCTCAGCCAGAAGCTGGAAGCTACTTTTCCTGCTGTGCGTTTACTCGTATGTATGTCCTTGGTATTCATATAATTTGAATGACTTTTTAAATAGTTGACGCAAGCCGTAGATTCTAAAGCTCGCTGCGTATATGTAATAAGTCATTAGCGTTCGGCGGCAAGCCTTATGGTTATTGTTTTAGCCTCTTTGCTAATGTGGCTTATTAGGATTAACCCTTTAGAGTTTGTGGGCGTCTGGTTTCTAGACTCCACTCCTCTCAATCATTAGTTTTCGCCATGTTTCTTCCTCTGTTTTTTTTTTTTTCTCTACCACACACACACACACGGTGAAATTTTCTAATGCACAAACACTTTAGACTCCCTTACCGTTGGCTTTATTTTAATTAAATTCTAATTAGAGACGAGTCGGTCCTTTTACCTTTGCTCGCTCGCTTCTCCGGCAGCTGCAGTTTGACATTTTGTGCCCGGCCACGTAATAGACAAAAACAGGAGTAAAATATTTCGAAAAATATTTCATTAACCAACCCCCCCATATCACACACACACACACACACACACCATACGTATACCATACATACATCAGAAAATATCGGGGGGCTTGGCATTCTATCTTCGAGGAGTTGCGCTTAGAAAGCGACCAAGTTGCTTGGGTCTGGCTTGGTTTGGCTTGGGTTTGATTTTTGAAGCTGTCTCGAAACCCGTTCCCTCTCGACTTCCCCTCTGGCTGACTGTCATG

 

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