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CRIMIC CR01166

Gene bowl

CG10021

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:3781077..3781092	24C4	FBti0202702	81202

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATAATGCCCACGGAAAGCTCATCCAG	AACGAATCCTCCTCGTCGCCACTTAA

Images

Construct Design Sequences

sgRNA sequence	CCAGCATTAAAATTGAGGGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCACCTCCGCCATATCTCAC	TAATGCTGGTGGAGGAGCC	CCAAGGGGTTGGGGGCTATA	GGGTGATTCCTCCATGTGCA

 

Amplicon 5'

Amplicon 3'

CCACCTCCGCCATATCTCACCGGACCGGGTCCATCGCCCACGGGAGCCGGAAGTTTTCCATTTCCACCAGGAGCCGCCGCCGCAGCTCTATTTCCACCTGGCCTGGGTCCTGGAATGCATGCCGGACTCGATAGGCGCCTGCTACGTGCTCCTGGTCGGGCATCGCGGCCTAAAAAACAGTTCATCTGCAAGTTCTGCAATCGACAATTCACCAAGTCGTACAATCTGCTCATCCACGAGAGGACACACACGGACGAGCGGCCCTATTCCTGTGATATCTGCGGCAAGGCGTTCCGGCGACAGGATCACCTGAGGGATCACAGGTGAGTCGAGGTTGGGGTACAAGTAATCACAAAAGGGGGCAGTGTCTGCTCGGGTAATTGCCATCAGGCCTGGAGTTATTCTTTGTGCTCAAAGTGTGAACGAATGGCGAATAATGGAATACCCTGTAAAGGCAAAGGATAATTAGATCAATGTAATTTATTGCTTACTAGGCAGTTTATACACACATTCTGAACAGGTGTGACAGTAATCTCGGCCAAAAGGGAAAGGCGACCACTACTCGTAGATGGAGTTTTATTGATTTTAGATATATCAGACAATCGCTTCAAAAGACCCCTTTTACCATAAGGAATTTTCCAGGAAATGTTTAAACTTTACGACGAATTTTCCTACCTCAACTCAAATAAGTAAGTCCCCATAAAGCGGACCCAAAATCAGAGCTCCTCTGACGGCCAGTAAAACTTCAACTCGGCCGCAGAGTACAACACAAAGTTAATTAACTCTGTTGGTTTTCCTTTGGGGCTCCTCCACCAGCATTA

CCAAGGGGTTGGGGGCTATATATCGGGTCTGTGTATCCCGGTGCCGATGCCCATGCCGATGCCAGGCAGCAGGTCCAGCATCCCGGCTTCAATGTGTCGGCCTGTCCGAGCGTTCTGTCTTTTGATCGTTCGGTATCGGTCCATTTGTCGTTTGTTTGCTTTGCCGCGGGGCTAATTCTTCTTAAGTTTTTTGTATTTTTTTTTTACCAGAAACGCACGGAGCATGGGAGAAGAATCTCGGTGCTTCGGAGGCAGAACCTGTTTTGGGTTTTTATACATTTATTTTTTTTTTTCCACCAGCAGCTGCTGCCTCCGATGCGATGCTGAATCTGTGGCTCCGTCGAAGATGGGGACTTTAACTTTTAACTGTGGCTGCTGCTATCCAACAGCAAGTGGACGCCATTGCCAAGATGGCCGCCATAAACATAAAAAATTAATGCAGCCAGATCGCATACACGAAAGTTAAATACCCTATCAGTTAAGAGGATACAAAATCATAGGATTTCTTTAATGAAAAACTTTAAAGAATTGGGTTTAAGGTTAAAACAAATCCAGGTTAGGTTTCATTTTGAAAGAGAAAGATAGGTATTATATGCTATAGTATTATATTATTTATTCTATTGTCTGCTCTAGCCAGTTATATCCTTAGTTAGAGTATTGTAAAGCTGTATATCAGTGAGCTTTATCGGTTCAAGTTAAGGCTGCATTTGAGCAGAGATAACTGTCAACCTGGGCTACCAATTGCAGCTACCAGGAAAACGCCCACGCTATAACAATAAAGTCTAATTGTTTCTCCACTTGTATTCGCCCAGGTATATTCACTCCAAGGAGAAACCCTTCAAATGCACGGAATGCGGCAAGGGATTCTGCCAATCGAGAACCTTGGCTGTCCACAAGATCCTGCACATGGAGGAATCACCC

 

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