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CRIMIC CR01169

Gene ft

CG3352

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:4209993..4209998	24D8	FBti0202703	81203

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK	AGAAAAGCACGCTCATGCCGACGAAT	TTGCTGACACAGTGACTACCTCTGTG

Images

Construct Design Sequences

sgRNA sequence	TAACATTACCCAGTTTAAGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGTATCGCTCACCTGATGGG	AGGCCAGGTTAAGCGAGATG	TTGACCCGCTTAAACTGGGT	TGTTTTGCTCCAATGGCTGC

 

Amplicon 5'

Amplicon 3'

GGTATCGCTCACCTGATGGGGAGTTTAAAGAATAATTTAAGAAAATACTCGATGCTGTATCTAAAATGGTTAGAAGATTTCTCACCTGTATAGTGGCATATGCCTCTGTGTACTGCACTCCGTCGGATACGCGAATCCTTAGCTCATACTTGCTCTTTGTCTCCCGATCGAGGGTATCGTTGAGGATCAGTTTTCCAGTGGACGGTTCCAGCAGGAACTTGCCATCGTGCCCATCCATCAGAAGGAACGTCACCGAACCAAGCGTATCCGGATCGTGGGCTCGCAGCGTAGAGATGGTGTGTCCAATCTGTAGATCCTCGCTGACATTGTAGACGAACGGTGTGTCGAGGAACTGCGGTGGACTATCGTTCTTGTCGAGAACGGTTACTCTGACCTGCAGAAAAGATACATACCAAAGTATTGCGTTGCGTCAATAACACTGACCCACTTGGCAGGAGGAGGAACCACAAACTGGCAACGGCGATATGGCATCGAGCGGAAACTTTAGTGCCATATTCGGCACTAAATGCCCAAAGTATTCCATGTACGAGCCTCCACTTATGTGCCCACCAAACTTTTGTCGCAATGTCAATCGCATTCTTCATCTCGCTTAACCTGGCCT

TTGACCCGCTTAAACTGGGTAATGTTATATGGTTTATGGGATATGCGAAGCCAGTTGCCGCAGCTTGAACTTTCTGCTTATGACCGAGCGAATTATAGTTTAGGCCACTTCTCTCTGAACGAAACCGGCTTGTTAACCTTTATTGCGGCACTTTAATGTTTATAATTATTTTTCACTAGTTCTAATTAATGTACAATTTTGCAAGTGCCTTTCTAAAGTCATTATGTGTACAAGAGTCGGAAAGGACGAAGTATCGATATGCTCTTAGCATTTTTGTAAGTCCAGCTAACGCTCTACAAAATCCCACCTCTTATTTCTTGATAGTGTCGCGCACATGGAGGGCAGAAGAAGTGGAGCCCATGGGGCCATAAATTCTCGACACCAGATTCACTCGTAAAATATATAGCGTCGTGTGCTGAGGTGATGGTGTTAAGTCGGTGCCGGGCGAGTAAATACTGCATTTGTAATAAAATTTATGCAAATTGTGTAACATCAGACGGCGGAGGCTAGGACCCAGCTAGGAGATCCACTTCCACTCACGAATGCATTAGTGGGCAGCCATTGGAGCAAAACA

 

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