• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01170

Gene dx

CG3929

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:6535035..6535042	6B1	FBti0202704	81204

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TGATCCTGAAGAATTGGCTAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCGGTATTTTCATCCGGC	TTCTTCAGGATCAGTGGTAAAC	AAGGATGCACCAGTCTGTGA	GTGGAGATCTCGCTGTGCTT

 

Amplicon 5'

Amplicon 3'

GTGCGGTATTTTCATCCGGCCTTATTTAGCCATTTTCCGCTGTTGTCTCTCATTAAATCGCTGTCCAACAATAAAAATTTCTCGCCTGCGCTTGCGCTAAATGCTAGAAAAACTGTTTTTACCATCAAACGTGAATTCTTAAGCTGCGCCTAAACGAAACCGAGTGACTAAAGAACCAGAACGAAAACTTCGGGAAAATGGAAGCCAGGGAAAATCAGGGATAACTAACGCTGGCAGCGGGTCCACCATTTTTAATTTCTTTGTTTATTTTGTGCCCATCTTCGCGAGCGAGCGAGATAGCGCGACAGCAACAGCAAGAGAGAGCGAGAGAGAGAGAGTGAGTGAGTGAGAGCTAGTGAAGAGAGCGCAGGAGGAGTTGGATATGGAAATGGGCATGGATATGGCAATGGGCTCACTCCACGGATAACGGATCAACTGCAAGCAATGGCCAGCAGCGCCGGAAGTGCGGCATCCGGATCCGTTGTTCCCGGTGGCGGAGGTAGCGCCGCCTCCAGTTGTGCCACCATGGCCCTGTCCACCGCCGGATCCGGTGGGCCGCCCGTGAACCACGCCCACGCCGTCTGCGTGTGGGAGTTCGAGTCGCGCGGCAAGTGGCTGCCCTATTCGCCGGCGGTGTCGCAGCACTTGGAACGCGCCCACGCCAAGAAACTGACGCGCGTCATGCTGAGCGATGCGGATCCCAGCCTGGAGCAGTACTACGTCAACGTGCGCACAATGACCCAGGAATCGGAGGCGGAAACGGCCGGCTCCGGCCTGCTGACCATCGGTGTTCGGCGCATGTTCTACGCACCCAGCTCGCCGGCGGGCAAGGGCACCAAGTGGGAGTGGTCGGGCGGCAGTGCCGATAGCAACAACGACTGGCGGCCCTACAACATGCACGTCCAGTGCATCATCGAGGACGCCTGGGCGAGGGTGAGTGCCGTGCAGAATCGAATGTTTTCCCATTGGCAGTCTAGTTGGAATTTTGAGTTGCTCAGGAATTTTTTTATCGGGAGAATATATGGGGAATCTTAAAAGAGTGTGGGTTTACCACTGATCCTGAAGAA

AAGGATGCACCAGTCTGTGATGTGTCCATAGGTGTTTAAATAGTACATATAAGCCAAATTCCACAGTTCACATCTCAGCCAAAAAACGTAGTAGATCGAAGAAGTGTACCTTTTTGAATTTCTGATGCTTAGACAAATAAATCTGCATTTAAATTTTTTAGCTAAAATCAAATTAATTTTTTTTTCAAAAATTTCCATTTTTGCAACCTCAAAATGGGACAAAAAAATTGAAAAAAAATTTAATTTTCACTTTTGTAAAAGTTTGAATACAGAAACTTTCTGCTTTAAAAATAATGACTATTAAAATCATTCGCACTTAAATCGCTGGAGTTTTGGCTGAGTTATGGTATTATGATCAAATTTTAGGGGCTATATAAATTTATTCTTCTTCTTGCACAAAGTCCAACTGCAACCGCATATCAATCAATCAATATCATCAATTATAGATTGTAACATAACTTATTCCTACTCCCTTGCAGGGCGAACAAACCTTGGACCTGTGCAACACCCACATCGGCCTGCCGTACACCATTAACTTTTGCAATCTCACCCAGCTGCGCCAACCCAGCGGACCCATGCGCAGCATTCGGCGTACCCAACAGGCGCCGTATCCCTTGGTGAAACTAACGCCACAACAGGCCAACCAACTCAAGTCGAATTCCGCCAGCGTGAGCAGCCAGTACAACACTCTACCCAAACTGGGAGACACCAAGAGCCTGCACAGAGTGCCCATGACCAGGCAACAGCACCCATTGCCCACCAGCCATCAAGTGCAGCAGCAGCAGCATCAGCACCAGCATCAACAGCAGCAGCAGCAGCAACATCATCACCAGCATCAGCAACAACAGCATCAGCAACAGCAGCAACATCAGATGCAGCACCATCAAATCCATCATCAGACGGCGCCCAGGAAGCCGCCCAAGAAGCACAGCGAGATCTCCAC

 

---

Search Again