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CRIMIC CR01171

Gene Spn42De

CG9460

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:6887511..6887511	42D6	FBti0202705	81205

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	short band

Images

Construct Design Sequences

sgRNA sequence	CTGCAGAGCATTTAGGTTATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGCCGACGAGTTGAAACT	GGCCACTAGTATTCGGGTCA	AGTGGCCAATAACCTAAATGCTC	CACACAGGAGGCCGATACAG

 

Amplicon 5'

Amplicon 3'

ACTGCCGACGAGTTGAAACTGGGATTGGGTCTGGAAGGAGCTGGAAAGACCGAGGTGGCCGAGAAATTGGACCAGTTGTTGGCCAAGGGACAGTGGGAGAAGGCTAGTGGGGACGAGGACGTGCCCAAATTGAAGTATGCCAATCGGATTTTCGTGACTCAGAGATTCAAACTGACCCAGACCTATCAGGATTTGGTGAGCAAGAACTTCGCAGCAGCTGCAGAGAATGTGAACTTTACCCAAAAAGCGGACACAGCCAAGCACATTAATTCCTGGGTGGAGGAGCAGACTCATCAGCAAATCAAGGACCTCATTGCTCCGGAATCTCTGGACGCAGATACTTCGGCCATCCTGGTCAATGCCATCTACTTCAAGGCCGACTGGCAGAGCAGCTTTCCTGATTACGCCACTTACGCCAGCGACTTCGTCAACCACGGAGGTCGGAAGGTCAGCGTGGATACTATGTCCCAAGAGGATTACTTTAGGTTCGGCGAACTGACCGAGTTGAAGGCCAAGGTCGTGGAACTGCCCTACACAGGCACAGATATCGTTTTCCTGATCATCCTGCCTCAGGAGGAGCAGGGACTGGCCATCGTCGAGGAAAAACTGATGGGCATAGACCTCAATGAAATAAGCTCCCAATTGAGAAGGCGAAAGGTACGCGTGCAGCTGCCCAAATTCAAATTCGAATTTGATGTCCCTCTACAGGCAGCCCTCGAGGAGGTGAGTCCTTAAGCCTAAGATATCTAAATACTAGATTGGTGACCCGAATACTAGTGGCC

AGTGGCCAATAACCTAAATGCTCTGCAGCTTTAAAATTCGATGTGTGCGATATGTTGTGTTGAACTATTTTTGAATGTTTGATATACCATAAATAGATTCGCCATGGCTGTCCGTTTGTCTGTTCTTATGAACCCCTTCAGAAGTAAAAGCTCGCGAAAAAGTTTTGATGGAGCATACAGACTCAATTTCAAAACATAACCACGTCCGGTAGACTTTTTGATAATATGTGTATATATAATATTTCTGAATATGTTTATTTAGAATAGAAAATAACTACTGATATACCAAAAACATTTTTGCTACTCCCATGAAGTACTTACATTCTTGAACAGCATTAATTTGGACTCTTTCAGTTGGGCATTAAGAAACTGTTCTCTCCTGGGGCAAATCTGAGCAGCCTGTACCAGGGATCGGAACCCCTACGCATCTCGGAGGTGAAGCACAAGGCGATCATCGAGGTTAACGAAAAGGGCACTACGGCCAGTGGAGCAACCTGTAAGTCTGTCCGTAAACATAAATATACGAACATTTTTGACAGTCTTTATTCCTTTCATTTCAGTTATCAAGGTCTCGGTGGAGTCGCTTACGATTGGAGAGGAAGTATTCGAGTTCATCGCGGATCATCCCTTCTTCTTTGCCATCAAGGATGCCCAAAACACCTTATTCCTGGGCCACGTCAGTCAGTTGTGAGTCGGCGCTGGAATTCCCCAATATGATGTTTTGTCCGCATCCAATAAATGCCAGTTCACGATGTCAAGTGCACAAGTACCGACAGGTATATCAACCTGGCTACTTGATTAGTTTTGGGTTGCATAATACTTTTGGCCTTCCTGTTTGCTGCTTCTGTAATTAGAGCCATCCGAGCTGACGTTGATAGTTTATTAAGAGGACTTGGCTCGGTTCAGCCTCCGATCCTGGCCGAGTGCCAGGCTCTTTGGCAGTCCTGTATCGGCCTCCTGTGTG

 

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