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CRIMIC CR01172

Gene mdy

CG31991

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:16823147..16823147	36B1	FBti0202706	81206

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CATCACACAGACCTCGGAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCGAGGATACCGCTCTGAA	CAGACCTCGGAGTTGGTTCC	CGAGGTCTGTGTGATGCCAT	CAGGCCACAATGCAGTTGAC

 

Amplicon 5'

Amplicon 3'

TCCGAGGATACCGCTCTGAAATATATGTTGAAAAATGCATAAGATTGGACTCTGTAAAATAAAATTTGTGATGTATAATATAACTTACCAGTTTACCCAAGGCTTGAATCTGAGCAGATCCCTGCTTGATGAAAGCAGAAATCAGGCGAATTAATGGTGTGACATTGCCCGTCCTGTCCCACAGAGCTGGTGACAACAAGCAGGGGAACAAAGCCCAATAGGGTTCCGGTATGGTACCAGTGCCCTCGCGCATTTCCAACAGCACAGAAAGCATCTGGAAGACGTAGGGCATAAATTCGACAATGTCCTGCTGTAAGATGCCCTGGAAAACTGGGAACAATGCCTCCTCAAAGGAGCTCACAGCCGAGGAATCGGCGTGACAAACAATTCTAGGGAAAAAGAAAATTTGTTATTGCTCTTTACCACCAAAATGCATAACACTTACTTTATACACAGAGCCAGCGTTTCAAAAAGATAGTGGTTGAACTGCGGACGCGATGGATTCTTGGCCACTTGGGTGAGAATCTCGGTAAGCCGTGGAAGGGCGACGCCCATAAAGGGCATGGCGGCCGATTGCAAGACCGAAAAACTTCGCATAATGGCTAATAATAATAATTAGAGTTTTGTTAACATATTAGAAACGTATGAGATAGTCCCCCAAACTCACCTTTCATTACGTACTCATTTTCACCGGAACCTGGGAGTGAAAGGGTCGCAAATAACCCACTAATTAGTTCAGTTGTGTAAGGTGCTAAAATCTGAGGACCAAAAACGATCGCGTTGCTAGCATCCCGCATAGAAAGGATCTTCTCCACTGAACAAGCAGCATAGCTATGAACAACAGAGCTTTCGGCGGGGAGATGTCGAATTAGCTGGGGCAGGCAGCTTGCTAAGACTTGGGGACCCAAAATGCTGCGGAACACCATTACATACTTAATTGCAGCAGCCTTAAGAACAGGAAACTCGTTAACTGCAAAGAGCATTGGAAGAATGCAGATATAAGATGATCGAATTGGGAAAATGGTTATTTGGGCTTACTGTTGGGTCTTTCCAGTTCTGGAATAATTTGCTGGGCACAAAATTCGGGAAGTGGAACCAACTCCGAGGTCTG

CGAGGTCTGTGTGATGCCATGCTTTTGAGTTCCTCCTCGAGATGCCCACGAAGTAACCAAATAAATTGCCGTATCCTTTGACCTCCAATTGGTAGCCGGATTCTCCTTGTACTTGGTCAACAATCTCTCTAGATACTGGCCAAAAATGCCAAAGATCTTTTGTTCAAAGTTGATTGATAGAGTCTTCACCAGATCACATGCCGCCCGACGTCGTGTGTCAATGTCAGAGCCTTCAATATCCCTTCGAATGTACTCCTCCGGACTGTCCTCGAAAATTTCTTCATCTGAGGGCCGGATGTCTAGATTGGGAATGACAACTTTATCGCAGATCTGAGCAAGAATCTCTGGGTTCTCGAAAATGCTCTGATAATGCTGGCGGTCAGCCACCACAGAGAGGAACTGCAGAGCATGGGAGACCAACTGTGCGCAAAATAAGAATGGAAAAATTATAAAAATAATTATTAAGAAAGCGTATGGTTTCTGTTACTTACCGAATCGTACTTGGTGTGCAAACTTGTCTTGACCAGTAACTCCCAAACTGCAGTCACAAATTGCTCCATAAATGGCTTGAACTCCTCGTCGTACTTTTTTGCATAAAGGCAAATGTTCTCGCAGACTTGAGCACGCAGATGCTCTAGAACACCCGCATCCTCATCATCTGCAGTACGCAGGGATGGCACATCCGCAGCTAGTTGCTGAATAAACGCTCCCATCCACGTATTGATATTGTCCTCAAAGAACTCCGGCAAATCTTGGGAGTTGAGCGAGAAAAACACCTTGTTCACAAGGACCAATGAACCGTAGATGACCTTCAGGGCGCCGGCATTGTTCTCGTGCACCTTAGTCAACTGCATTGTGGCCTG

 

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