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CRIMIC CR01173

Gene CG2970

CG2970

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:23951132..23951150	60A13	FBti0202707	81207

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACGACCAGTCAATAAGGTTCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAATCCATAAGCAGCGGCAC	GTTCAGGTAGAAGTCGTCTTGC	GAAAAACACCCTGGCCGTTG	ATCCGGACTCACTGACAGGT

 

Amplicon 5'

Amplicon 3'

CAATCCATAAGCAGCGGCACGTCACCACATCTGTTATATCACCGTTAAGCCTCGTACGGCGTGGAGGATCCGGAGTTCGCCATAACACAACTGGCCCAGACGACGATGAGATCGGAGCTGGGCAAGATGTCCATGGACAAGGTCTTCCGCGAAAGGGAGTCCCTCAACGTCAGCATCGTCGACTCGATCAACAAGGCCAGCGAGGCGTGGGGCATCGCCTGTCTGCGATACGAGATCCGTGATATTCGAGTAGGTCACATCCTTCAGTTGACTCTCAAGTAGCGAGATTAATGGATCGTGTCCCTTTTCAGCTGCCCACCAGGGTTCACGAGGCGATGCAGATGCAAGTGGAGGCCGAGCGGCGAAAGCGAGCCGCTATTCTCGAATCGGAGGGTGTTCGCGAGGCCGAAATCAACATAGCCGAGGGCAAGCGGAAGTCTAGGATTCTAGCCTCCGGTAAGCGGCAGTGTTCCTTACAGAATGTACCCAGTAGTTACATTCATGGTTCCTTCTTCCACAGAGGCGGAGCGGCAGGAGCACATCAATAAGGCCAGCGGAGAGGCGGCTGCCATTATAGCCGTGGCGGATGCCAGAGCCCGCAGTCTACTGGCCATAGCGAAATCCTTATCCCACCTGGTAAGTATTTCGCCGGGGGACTCCACCAACTCTCGATTTCGGTTAGGTAGAATGCTAGGCATTTAATTTCAAAAACACTTTGGCATCACACTACGAACAAATATTGGAATTCGGAGATGTTTCAATCAGAGTATCAGCAACGATTGCTTCCGGGCGTCGCCGTCGGCAAAAAGGAGGACATCGATCTGCGCTGCATTCCCACACGAGCTGGTAACCACTGGAACCGGAACTTTGTCAATGCTCCGGGAGACTCCAATGTGTACGAGAGCTCTGTGTACTCATGCCTCTTGCGCAACGAGTTCCTGGACGAGAACATCACTTCCTTGGAGAACTGCGAGCAGACCGCACAGGTCCATGCCCAGACTGATGATTTTAGGGATCGGCATAACCGTATCTTTGGTTTTGCCAGCGGTTCTTCGTCGCCAGTACGCACACCATTCGCCAACATCAGCGAGAGCAGCCAGCGGCTGCTCAGCATCTTCAAGAGACCCATGCGCCGACTGCCACGTCGCCCGTACAAGATACTCGATGCGCCGGAGCTGCAAGACGACTTCTACCTGAAC

GAAAAACACCCTGGCCGTTGGTCTCGGGTGCTCGGTTTATCTCTGGAGTGCCGTCAGTGGTCAGGTGACGCGACTATGCGACTTCAACAATGAGGATAACCTGATTACAGCAGTTAGCTGGCACGGTGAAGGCCGGCAGGTGGCCATTGGCACCCAGTCCGGGTACGTGACCATTTGGGATGCCGAGAACCAAAAGCAGATAAACCGACTGGAAGAGCACTCGGCGCGTGTAACGGCGCTAGCTTGGTGCGGCAACCGATTGGCCAGTGGCTCCCGCGACCGCTCAATTTTGCAGCGGGATATCCGCAATCCGCCGACGCACATAACCCGCTGCTTGCGCGGACACAAGCTAGAGGTGTGTGGCCTACAGTGGTCGCCCAGCAATCGATACTTGGCCAGCGGTGGCAGCGACAACCGCCTGTTGGTTTGGACCGACGATTGGCCGGAGCCCATTTACGCCTTCGACGAGCACAAGGCGGTGGTCAAGGCGCTGGGCTGGTCGCCACATAAGTCCGGTCTTCTGGCCAGTGGCGGTGGGTCCGCCGATCGCTGCTTGCGCTTTTGGAACGTCCTCACCGGGAAGCTGGTGAAGTGCATCAACACCGGCGCCCAGATCAGCAACCTGGCCTGGGCCAGGGATTCCCGAGAGCTGGTTACCACCCACGGACACGCGCAGCCACAGGTCATTGCCTGGCGCTATCCCTCGCTGAAGCAGATGGCCAGGCTGTCCGGGCACACACAGCGGGTACTCCACCTGTCAGTGAGTCCGGAT

 

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