CR01174

CRIMIC CR01174

Gene CG10257

CG10257

Information

Donor Location Cytology FBti Stock number
pM37-p02R:14943100..1494313751D5FBti020270881208

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence TTTCCATTAAATGTAAGTGGCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GAACGGAACAGAGCTGAGCT ATCGCAGGTACCTTGCCATT TCGCGATAATTATCCGCCCA GTACTGGTCGTGCGATTTGC
 
Amplicon 5' Amplicon 3'
GAACGGAACAGAGCTGAGCTGGGCTGCCGGAGAGCCCGGCCAAAAGGCAAAGGCAGCCAGCCGGGCCAACTAGTTTTTCTTTGGCTCCTCCTTTTGGCTTTTATTGTACACGGTGGTTTGTTGTTTAGTGTGCCATTGGCGCTAACAACGTGCGGTTCGGTCTTGGGTTTTCTCGCCGCCCGTTGAATTCAGAGAGCGGAATAGATTCTAGCCCGATTTCGGGGTCTAAGCCCACAAACATAGGGGTGGAGTGAAAGTGCATTGCAGCCAGATCTGCAGATTAGACAAACTTGAGTTCCTTGAACTTCCCAAATATTATAGATATATTATACCTACGTGGTTTTCTCTGGTTTTGTGTAACCCATTTAGTTGGCTGCACTTTAAAGGCGATTACTATAAATATTAATAGGATTTATTGATTGACTGGCGGTGGGCCAGAGCCAGATCGACACGAGGCTTCGGACCCATGTCCTTTCTTTCGCCCACACTGCGCCTGGAGAATCTGTCCACGCAGCCCACGATGACGCAGGATCATGTGCCCGAGGACCAGCGCTATAACAAACAGAATATTGTGGCTCAATGGTGTGTGCCCATCAATGGCAAGGTACCTGCGAT TCGCGATAATTATCCGCCCATTCCCATTTTTATTTTTTGTAATTTTTATTTTTTTTGCGCAGATGTACCGCATCGAGCTGGAACATGGCACAACCAGTGGGCGGCGCATGATTTGGGTCAATGGACGGGTAAGTTTTTTAGCTCCCAGCGAGCTGAATGGGTGGTTGGTGGCTTTGGAGCTCGTAAAGTATTTGCATTGTGAAGGTAAACTTAATTACGGGTCGTGTACGTACGTAATCGTACTTCAGCAATGCAGAATGATATTTAAAAAGGTCTCATTTAGTTGATAAAAATATTTAATTAAATTCTTTGGGTCTTATTATTTCTAAACATATATGTATATACTTCTGTCATCCCTCACCGACATTTAGCATTTCAGTTTTTATGTTTAGTACACCGTTGGGTTTATTCTTTCTTACTAAAGTGCTTTAGTGCTTTAGAGTGCTTTTAAACTTTAAGTCCATATGAACTGTATAAATTAAATCGATACAAACAAACAAAATAGAAGATTTTACCAAAGACAACGGTGCGTATGAGTGATAAACTGGTAGAGCAAATGCAACAATTTAAAGGGCTATCAAAATGAACTATAAAGTTCATCCGCCTAATTTTCTTCTCCTAATCAAATTCCCAGGAAGTCCTGCGTCGCGACTGGATGTTCAAACTGGTTGGCGAAGACACCTTTCACATCGATCAGACGCGCTGCATCATACGCGTTGATCCTGCGCCGGGCTTCAAATACGAGTACTCCCTCTACATCGACGGCAAATCGCACGACCAGTAC
 

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