• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01175

Gene NLaz

CG33126

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:1361372..1361411	22A1	FBti0210325	86338

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CATTCTGATAGATTTAGAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGACGGCGTATGACTCGTAA	TCAGTTGCAAGCGAACATGC	ATGATTAACATGCGATGCGAG	TCTCCTGTGGGTGGATGCTA

 

Amplicon 5'

Amplicon 3'

CGACGGCGTATGACTCGTAATCTGTGCCCAAAACCAGGTAGTTGGCCTTCGTCAATGGCTCTAAAATGTATTGGATAAGCAAAAAAATGTTTGTTAAAGAGACCACCTAAAATTGGCATTACTCACGCGTCGGGTAAAAGGCCACGGCCAATTGACCGGGTCCAAGGACCTTGGCCTGTCCAGTTACATTCGAGGGTTGTCCGGTGCTTTTTCATCGATTTTTTGATTAAATGGAGTGTTTCAGATTATACTACTTGATACTCACAATCGATTGATGGCCGCATTCACCACCGAAACAGTGCTGTTGTCTATGAGACTGTAGTTGGCGTAGATGCACTTCTTGCCGATCTCGAAAGCAAATGGATAGGCTGCGTACTCGTACCAGACGCCCATATACTGAAGTATTCGAAATAATAATAGTAATAAATACAATGTAAGAATATTACGTTTAAGCAGATGGTCATATAATAAGTAATACAAACTACAGCACGAAGTGTTTACTTGTTATTTAAAACTGTGGAAATAATTATAGCTACATTAGATAAGTAAGGTCCGATAATTAAAAAAATACCAAATACAAATATTTACCTAGAAAGCTGTAAGAACAGTGCACTTTATTAATATGAAAGCAATTTGCTTATCTTATCATTATAGTTTTCGCTATCTATCAATTTTGCAAAACTAACGAAATTTTAAATTTGAAGATCGAATTTCCCCAATTGGTAAACCATGTGAAGTTTTACTAATATCGCTTAATTCGATATATACCCTTATATTTAAATTTTAGTTCATAACTATTGAGACTTTGAAGTGCCTTTATTGGCATGTTCGCTTGCAACTGA

ATGATTAACATGCGATGCGAGATGGTTGGCCTAAGGTCAGAACTCACCGCTTCCGCGTCGAAGGTGTCCAGTAGCTTAACATCTGGGCACTTGCCAGGAAATGGCACCTGGGCATGGGCCACCCATACTGCCCCAAATACCACGGAGATAAGCAGCAACAGGTGCGAACTGTGGGGATGATTAATGAAATGTAAATAACTTGGAGCTAGTGCTCAGCGAGTGGCGCTTACCTCGAGTGATGATTCATTCTGGAATATACTGGGAGATACGGTCGCTATCCACTGAACTTGGTATCCGTTCTACTTCTGGCTTAGAACTGAGGTTCGACTGCATCTGCATTGTATCTTTTATACGCTGGATGCGAGAGCTTTGCTTTTCGCGAGCGAGCTTTTCGAGTGCGCCGAATCGGATTGCCCATGTGAACAAAGTGAAATGCAGACGATCTGATTGCAGAATCAGAACATATTGCTCTTGTTACAATCGCTAGGAGGCAGCTATGGAAAACAATCCCAATACAACTCACTTAATTTCTGTATGCACATCGCGATAATTCGCTTTCGATAAGGGCTTCCGCATGCAAAGCGGAACAATTTGCACTGCAATTACGGGGGTCAATTAATTAAGAGAGATCGCAACTTAGTAGGTTCAATGCCCTGATATGGCAAAGTGAAATTGAATAGTGAGTAAGCAGAAATGAATTATTCAGTTCGCAAATCAGAATGACTATTTCAGTTATTGCATACGTATTAATCAGACACCTTTAATTTAAGTTACATCTTAACCATTTATATAATTATTTATATTAATACCTTTGCATATTCAATTACTTCCAAGTATTACAAGTTTTATGACACTATAATAAAAATGTATTTCTGCTACACTGATTTTACTAAAATATTTTAAATTTGGTACCACTGCAATATTTAAATAAATAAATGGTATTTCTTAGCATCCACCCACAGGAGA

 

---

Search Again