• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01177

Gene sd

CG8544

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:15822679..15822679	13F4	FBti0206938	83253

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GAGAAGGAGTATGACGGTGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGCCTTGTGCAAATCACTC	GAGTATGACGGTGCTGGGTT	CCGTCATACTCCTTCTCCTCC	CGGTTCGGTTCGTACGTACA

 

Amplicon 5'

Amplicon 3'

CCGCCTTGTGCAAATCACTCGTATGCTGTCATACCGCGATCCAAAACAAAAACCGAAAAGCCGAAAAAGCGAAAAAGCAAAAAAGCAGAAACTGAAACACACGCAACTCATTTACCAAGCTACTGCGCGCCTACTGTTGCATCGAAAAAAAAAAAAAAGAAATGAAAAAAAGAAACACAACTGCACAAGAGAATCATTGTAGCTCCACAACTCCGACCACCGAAAACCCATCATCACTGCCATAATCCGCAATGCTCATTTAGGATTTGCAAATGTCTTGTTTAGCTTTCTGTTTTGTGTGTGCTCACCCAAAAAAAAAACATCAATGTGCCGGAGGAACCTCTGGCCCAGCTTCTCCAGTCTCAACAGATTTGAAATTGCTCAAAGAGGTGTCTTAGATTATTGTGCTGTTCGATGTTATGCCAACATCCCAGTCTAAAGTAGCTCGTTATTGGGAACAACTCGTTTCAAGAGATTTCCAATCTAAAGAACTTTGATTCCTTACAAGAAGATGTTTGTTTTTAAGTGGAATTCAACTCTATATTAACGAAGCTCACTTTTAGAGACCTTTTGCATCTTCTGAGGCTTTAAACCTTATAAGAAGGTTTTTAAAAGTGTATCATCTTAGGACCACTAACGTAGACACCTCTTTGAATGAGATTTCGAATCCTCGGAGGCTTTTGGGTGTCCATAAGTACGCAACGAAGAATATGGCATATTCGAGATATTCTATAGCATACTTTTGGACACTTTGTTGATGAAGGGATTTTCGATCTATCGCACCCGTGTATATAACCAATGTCCTGCCATCGACGAGGAACTTCACTTTTCATCTCAAACAACCCAGCACCGTCATACTC

CCGTCATACTCCTTCTCCTCCTCCTTCTGTTTTCATCTCTAGCTAGCTAGCCATGCAATCGAACTCCCCAACCCTATCATCCGCCCAAACCACTCGAACATCAAAATTGCCCATAAATGAGATGCCCGTCGTACTGCTACGCCAAAACACCAATGCGCTCGCTTGCTCATTTGAAATAGACTCCTTGCTTTTGACTTGCTATGCGAAGATAGAAAGTAGCTTCGCCCAAAAAAAAAAAAAAACAATTTCAAAGTTCGCTATTAAACTGTGTGTGTACTATCGTATGTAACAAACATACTCGCTGGGTAACCTTAGTTAAGTGATACAATCAGTCGGTTAGTGAATAGTTGATTAACAAATCACCTCTCACCATATATGTCTAACCACTCTTGTACATCATCTACTGACACCGATGGGTCTAAGTTTTAAAGCTAGCCTAATCATTCGATCGAAACGAGTATATGAAACTGATTTTAATCACTTGTACATTTGCACTTTTTCCAACAGCAATTCTGGCAACCTGGACTACAGCCAAGCACGTCCCAAGAGTAAGTTGGCATCTCGTCGTCTACTTTATGAGCTAGCTATACAACGCATTTGCATTGACCAATAGTTTCTATGATTACAGCATCAAGCCCTTCCCCCAGCCGCCGTATCCAGCTGGCAAAACGTCGACTGCGGTTTCCGGGGACGAAACTGGAATTCCGCCCTCACAATTGCCCTGGGAAGGACGAGCCATTGCCACGCACAAATTCCGCTTACTCGAGTTTACGGCGTTCATGGAAATCCAGAGAGATGAAATTGTACGTACGAACCGAACCG

 

---

Search Again