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CRIMIC CR01178

Gene cutlet

CG33122

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:3708818..3708827	24C1	FBti0202710	81210

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CACTTTCCACAGGGCTTGGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCTCAGACGCACCTGGAAA	AAGCCCTGTGGAAAGTGCAT	ATGTTTGCCCCGGCTAATGA	AACGATGTCTACGATCCCGC

 

Amplicon 5'

Amplicon 3'

GTCTCAGACGCACCTGGAAAAGCACTGAGTGCATCGAGATCCGGTTCTGTTTGGAAGACATAGTGTCCTTCCTGGGACTTGACCTGCACAAACGTTAGGCCGAGATCAACCATCACCTCGATGGTGTGGCGCAAGTCCTGCTGCTCCCTGAGGTCATTTAAATATGCACTTATTGGAATGTTCCAAGGTAATCCGGGTATACTCACTTTGGCGATAGCAACTGCACCGCCACGGAGCGCAGTTGCGGCGATAGAATCCTCTTTAGCAGCGGCACCGTATCGAGAAGCAGAGTTCCGCCCTGACCCACACCCAGCGCAGAGGTGGTGACACCTTTGCAGAGGGCCTGAAAGATGTTTCTCTGGTTGGTGCTCTTCTGCTGGAACTGCAAGGATACAAAAAGAAGGGTTAGTCCTTGGGCAAACAGTGCGAAATCTATGCAGGAGTCTCATTAATCTTGTGATTGCATTTGAGGCACATAATGGACAAGAAATTACATGGCTTGATTGTGTTGATTATGCACTTTCCACAGGGCTT

ATGTTTGCCCCGGCTAATGACGCCTGCATAAATCATGAGCCTGCCTCAAAGAAGCAGTCTCTCTGCGTTGAAAAGAAATAATGTGGCCCGGGGCCATCATCTCCTCATCGTCATCGTCATCGTTTCGTTTCTTTTTCTTTCAGCAGCTCACCTCAAAGCCACGCGTGGGAAATGCGATTTTTGGCCAAGCCAGTGTGGCGAACAGCAAATGCCAAGCGACGAAACCGTACTGCAGGTACGGGTACACACTGTAGTTCTGCTGTCTGCTGATCTGATGCTGCACTGTATCCGTGAAGCAAAACCATTTTAGGGCTTCGCACACGCCCGTAAAGTTTGGATCGGGCATCTTCTGTTGCAGGTAGTTCTCATACACGCCTTGCGTTAGTCTAAAGAGTGAAGGAAAATATAAATATCATTCGTCTATTTAATATAAGTTAACGTGCGATTACCTTTCATAATCCCCACTAGAGTGGACCACCTCTAGTACATTGCGAACTCGTGTTGTCACAGACGTGTTTGTCATGGTAACCTGGGCGGGCTCATCGACCTTATTGGCGTCTGTGTGCAGGGTTTTCCGCGGACGTTGTATCTGAAAACAGAAATAACATTAAATAGCACCCCACAAACACGCAACATACAAAAGCGCACCCGAAAAATAGCATCCCACACGGCGAACAGACCTTGATGCCTGTCCTTTTGTCCCAGATTGTTGTTCAGCACATCTTGCAGAGTAAGACTGTGCTTTTGAGCACTACAAGTTGAATTATCGTTAGTATAGTAATGAGTAAGTATTTGCTAATTACTCACTTAAAAAACTGCATGGATGATATACAGCTCCTCACATCGTTTCCGGATTTCTCAGCTAGCGCGATAAGTGAACCAAAGTCCGTCTTAAGCTGCTCTTTGAAGGCAATCTTAACTAATCTGAAAGTAAGATATAATTGCCTTCAGCATATATACTATATTTTAAATATATATAAAAGATATTGCCTCTCTGCCAGACGTGCTGCATCGATGGGCGGAAACGTGACCACAAAGGCCACCTGCCTTAAAGGGCGCAGGGCGGGATCGTAGACATCGTT

 

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