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CRIMIC CR01182

Gene CG17593

CG17593

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:3515308..3515315	23F6	FBti0202711	81211

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CGATGAGAAGAGGTCAAATGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGCGTGTGCTTATTGGGAA	CTCTTCTCATCGAACAGCCGA	TGCGGTTAGGAACTTTGATCA	AAGTTCTCATCCAGCAGGGC

 

Amplicon 5'

Amplicon 3'

AGGCGTGTGCTTATTGGGAATACCGAATTGCATTCAAACTAAAAGGCACTAACGCGTTTTATTCTTCAATCTGCACGCGACTGTCACACTAACCATTTAACCTAGTTTTTTTTTCTTAATTAGTGTGAAAAGTTTTAGGACATTTAAATAAATTATAACTTAAGTGAACCGACTAGATTACGCCATCGTACTACCCCCAAGATGCGACCAGTATTTGCCTTCCTGCTCCTGGCGCTCATCTGCTTCGCCAGCACTAAGTCGGAGAGCGACTTCGAGGAAGACGACAGTGATGATTTTGCCGAATTCGACGACGGCAATTTTTACGCTGAGCCAGCGGCCAAGAATGCAGATTCGTTCTCCGGAGGAGCAGGATCCAGCCACGGCGAGTCCCGCGACGATCCGCCTGCCAAGGAGCCGCGGAAGCAAGCACAAAACCAGGAGAGCGACGAGGAGGACGGCCTTGTCGAGGATGACGATGAGTTTGAGCACTTCCAGGTAAACAAAGTCGGCTGTTCGATGAGAAGAG

TGCGGTTAGGAACTTTGATCAAGAATATGCCCAGTGAAATGTAACTAGGCTTTCCTATAGGTGGCCTTGTGTTCCTTTACTTCCTTCATAAAATACTTGGTCATAAGGGTGCATAAAGAACCATTGGGAACATTTAATACATTGCTTTTAAACAGCTTTCACCATGATGACATGGAATTTTGCCGTACCTTACTTTAAATCTTCTTTAATGTGAAGTGCTTCTACAAATCTGTAATCGTTAGATAGTCATTGAATTATGTAACGTAGAAAGCAGTTGTAGTTTATACACTTTGTCGGAACCCTCATAACTTGTACTTCGAAATCAGGGAACCACGGGTTCACCAAAAGAGCAAAGGTGTAACAAGAAATTATTTATACCTACAATTGACTTAAACCTATTTCCAGGATGAGGAAGAGTTCGAGGGCTACGATGGCGGAGACTCCAAGGATCCGCCCATCGACCAAAAGGTTGAGCCAAAACTGAACATACCAAACATCCCTCTGCACTTCCGCACCCACTGGGACTCCTACTGGATGGAGATGCTCATGGTGGCAGGGTTGTTGGCCTACTTCGCCAACTTCTTTGCGGGAAAGGCCAAAAACGCGCGTTTGGCGCAGCTTTGGTTCAGTACCCACAGAGCCCTGCTGGATGAGAACTT

 

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