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CRIMIC CR01187

Gene sut2

CG17975

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8087495..8087508	44A4	FBti0206884	83255

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AACAAACTCGTCCCGGGTGAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGACGCGGAATCACTGGATA	CCGGGTGAAGGGGAAAGTA	GCTTAAGTCTTTCGTTTGCGC	GATGCAGGTGAACACTCCGA

 

Amplicon 5'

Amplicon 3'

CGACGCGGAATCACTGGATACTCTTCCACGGCGGGTGCGTTAGTTAATTAAGTGCCAACGCTTGAATTGTTTAGCAATAAGTGCTTAGCATTTGGAAGGCAAATATATCCTGATCTCCCTATATATATATGCCTGTGGGTAGGTTAATAAAATGTTGTATTTTCCACTACGAAAGATCTCTGATCAATTCGTAAGAGTATATAACTATTGACTTATATTGCCGGGAAATAGTTGGAGCAAACAAACGCTCCCATCTAACGATAAGAAAACAAGTATTCGCAAGACAAAAACGCTGAATTTATCTATGGCTGGTTATATGCTGTAAATGTATTCCAAGTCCGGGGCAACCAAAGTGAAGATGTGTTTACGTGATTGATATACAAGCGGTGTGGGTGACTCAACAAGCTGTCGAATGGCACTGAGTGAACCGTCGGATGCGAGTCACTTTCGGTCTAGCTTTTGGGGTGCCAGCATCGTTCCATCGCCATGGTTGTTGTAATGGTGATTCAAAACATCTTCTTGATACTTTCCCCTTCACCCGG

GCTTAAGTCTTTCGTTTGCGCTTTTTGCAACGTGCTAATCATGTAAATCACTCTGCTTTCCTTACAGCACGTGACTCCTCCAAAAGTCAATGGATGGACCCCGCTTCTGATGCTCATCTGCCTGACCGTAACGCTGGGCACCACCATTCCAGTGGGCTATTTCTTTGGGGTTCTCAATGCCCCGGCCGAGATCATCAAGAAGTGGTGCCAGGACATATTGGCGAACGAATACGACACTATCGTCACTGCCGGTCAGCTTGATATTCTATGGACTAGCATTGTGTCCATCTACCTGATTGGAGGCATCTGCGGATCCTGCTTCAGCGCTGTGCTTTGCGATAAATACGGCCGGTAAGGTTTAAATATAATATGGTGATTGGATTATATTTAATAGCTTATCGTTCCAGTAAGGGATGTCTGGTTATCAGTAGCGTACTATTCGTCGTTTCCGGCATCCTGTTCACCTGGTGTCGAGCAGCCAAATCGCTGGAAATGCTGATGACTGGCCGCTTTCTAGGCGGCATCGCCTCCGCTTTGATCTTCACAGCTCAGCCCATGTATCTACTGGAATCCGCTCCATCGGAGCTCAGTGGGAGCGTCGGAGTGTTCACCTGCATC

 

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