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CRIMIC CR01192

Gene sl

CG4200

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:16352221..16352221	14B17	FBti0202713	81213

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCATAAAAGGTATAAGTCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAAGCAAAACGACCTGTGG	TGGGTCATAAAAGGTATAAGTCAA	CCCATTACAGGTTTTCGGGGA	GCCGGTATAGACCCTGTTCG

 

Amplicon 5'

Amplicon 3'

CCAAGCAAAACGACCTGTGGAACAGCAAGTACGACCAAGTGTTCATGGACATGAACCTGCCGTTGTCCTCCTACTGGATCGCATCGTCGCACAATACCTACCTGACGGGCGATCAGTTCTCCAGTGAATCCTCCTGCGAGGCCTACGCCCGCGCTCTTCGCATGGGCTGCCGTTGCATCGAGTTGGATTGTTGGAACGGGCCCGACAATCTGCCCTACATATTTCACGGCCACACCATTACCTCCAAGATCAAGTTCATGGATGTCATCAAGACGATTAAGGATCATGCGTTCACCAGTTCCGAGTATCCGGTAATATTGTCCATAGAGCAGAATTGCTCCCTGGAACAGCAGCGGAATATGGCGCAGGCCTTGATCGAGGTAAGTTGACCTAATCGAATCTATTAATAACAACAATTTATATATTCCATGCAAACAGGAGATCTGCATGTTTGCTTTTTGATTCATTCGCTGTAATAATGAGTTCTTTTATGGCTTTCTGTGAGATTGCCTAACTTAATATATAATTGAAATGGATTTTGTATATTTCGATAGTTGGGAATATGTATGACAATATGAAGAATTCGCTTTTTATTCGTTTGAATCTAATTATAAATGTAACAGAGCAATATTTTTAGTTACTTAGATTTCAAGACGATGGTAATTAGATATTTCTTGATGCTTTAAGTATTAATCGCATAAAAATCCATTGACTTATACCTTTTATGACCCA

CCCATTACAGGTTTTCGGGGATATGCTGCTTACGCAACCTTGTGATCGAAACGAGCAGCACTTGCCATCGCCATACCAGTTGCGTCGCAAGATAATCCTGAAGCACAAGAAGTTGCCGCAGTTTGACGACATCGCGAATGGGATTTCTAGCACGGGTTCACTGGGCCATAGATCATCGTTGGGCGGAGCGGGCGGTGGAGCGCATGGGGAGAACGATGGCGAGAATGTGCGCAAGGTGTTCAAGGAGGGACTGCTCTACTTCAAGGATCCGGTCGATAAGTCCTGGAATCTTTATCAGTTCGTTCTGACCCATCAGGAGCTAATCTACTCCTCTGAGATCAACGAGTCACGCAACGGAAACTCCGAGGATGATGACTTTGGGCTTTCTTCGTCGTGCTCCCTAAACAGCAATATGCAGCAAAAGCAAAAGGATACCTCGGCCAACGATGAGCTGCACTTCGGCGAGAATTGGTTCCATGGGAAACTTGAAGGTGCGTTTATTGGTTGACATATATGCAGTATATTCCATTTGTTTCGTTAACAATTTGCATTTTAGGTGGCAGAAAGGAGGCTGATGATCTACTAAAGAAGTACAAACACTTTGGCGATGGTACATTTTTGGTTCGGGAATCGGCCACGTTTGTGGGAGACTATAGCCTTTCGTTCTGGCGACGTAATCGCCCGAATCATTGCCGCATCAAGTTAAAGCATGAAAATGGATCGATCAAGTACTATCTAGTGGAGAATTTTGTATTCGATTCACTGTACTCACTGATCGTTTATTATCGCAAGAATATGCTGCGTAGCTCAGAGTTTTCGATTATACTGAAAGAGCCAGTTCCGCAGCCGAAAAAGCACGAGGATCAGGAGTGGTTCCATCCGAACACCACCAAGGAGCAGGCCGAACAGGGTCTATACCGGC

 

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