• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01194

Gene IP3K1

CG4026

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9787061..9787064	30D1	FBti0206885	83257

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK (weak)	OK

Images

Construct Design Sequences

sgRNA sequence	CAGTTATCGCGGCTGCGAACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCACTGTGATGCCATCCACC	AACGGGTTTGCCTCGATCTT	CAGCCGCGATAACTGCAATT	GCTTTAGTCGCTTCAGCAGC

 

Amplicon 5'

Amplicon 3'

TCACTGTGATGCCATCCACCCCTTTTTCATCCCTTCAGCTCAGTGAAATGGAACGCGTGACGAATTTGTGCCACATGTGCAAATTTGCCGACTTACGTAAAGATCGCTCGCCATTCGGGGCAATGCTATTAGCCCAAGATTACGGGCGTTGGTAGGGAACTGCTTAAGAATACGCAGTGTTATCTGGCCAGTTAAGATACGATAATAGTGGAGAGATAAGCGCAAGAAAACAAGAGCAGAAACAGATAGGCGGCCATATAAGATGGGGGGGCCGAAACAAGTTCCGCGGCATTTACCTTAATTAGTCAATTAATCAACAGCAACGGAGTGAGGATCGTTTCGGTTTGTATCGGATTGGTTTTGATCAGTTCATATTGGCTCGGATCGCCTTCTACGCTGACAGATCGTTATTTGTTATGGCCAATGCTTATGGCTCAATTCGCATAGGCAGCGCAAACAAAAACAAACGCCAACTTTGCATACTAAGTGCCTCATTAGGGACACGGCAGTGCCGGGGATCTCGGTCGTAAACCACACACGACGACCCATCAAGTGGCTCCGCATTTTTATATAAGCATAAACAAAACGGGCCGCCTAGAATTTTTTTTGCCATTTACCCATGCATTCGGCTTGAATAATTAATTAACGCCTTCGTGAGTCACAAAATGCATTGCAATGTTAAATATTTTGTATCACCTTCTTGCACTGACTAACAAATTTATTCATTTCCTTTGTTACAGCGGATACCCAACGAGAACTGCGACTATTTGAGTCTACAGAGATCGGGCCAGGCGCCGAAGAATCACATCCAGGCGCAGGATCCGGCTCAGATGTCCCTGCTCAAGTTCTTGGCCATTGTAAGTACCCCATGTTGTTAGCACACTCGTCTCGTGGCAATTGGCCAGAAACTTTCCACCGTAGCGCTTCCTGGTCAGGAGCCTCGGACAATGTGCGATCGGTTGGCCTGGCGAGCTGCCTGCATAAATTTCACGTTTACGAGGCTGTGAGTTGGGCGTGGCCCGTCGGCTGGCAAATAAAATTCGTTTGATTATTTATAATCGCTGCCGGCGGACAGTAATGTTTCCCTCTGGCGAAAAAGATCGAGGCAAACCCGTT

CAGCCGCGATAACTGCAATTTATCAATGAATGCGTATTTCTTGTTATCCTTTACTAAGTTAATTTTTTTCTTCTTTCGCTTTCCAGAATGCCCTAGAGCTGAGTGCCCCAGCAACGCCACATCTCCTCCAGCATCAGCAGGCCCACAAGCAGGCGAAGCCACAGGGCTGGATGCAGCTATCCGGTCACCCAGAGAGGTAAGGATCTTACCAATAACTTAAATTTTGTTTTGAAAACAAACATTTTATTAGTCAATTTACTAACTGCTACTATTATTCTTATTTTGCAGCATTGTTCCCACATCGACTGGAATAGTGCGCAAACGGATCTCAGGACTGGAGGATAGCGAAGTACATGCCTACCGACTGATCTGCAAGGAACCACAGACCGCTCAGATAGTGCCCGCCTACTTTGGAATACAGGAGATGCAATCACAGCACTTTATTGAGCTGCAGGATCTGCTGGCTGGCTTTCGGGATCCGTGTGTGATGGACATCAAGATGGGCAGCCGCACCTTTCTCGAATCGGAGGTCAGCAATGCCACGCTCAGACCGGACCTCTACCAGAAGATGATCGCCGTGGATGCGGGAGCTCCCACGCCTGCGGAGCACGAGGCACGAGCGATCACCAAGCTGCGGTACATGACATTCCGGGAGTCCCTGTCCTCCTCCCACTCCAAGGGTTTCCGGATCGAAGCACTGCGTCTGCGCGGACGACCGCCTGTCAAGGATTTGAAGACGTGCCGAAGCAGCGAGCAGATTGCCCAGACGATCGAACAGTTTCTGGCTGCTCGGCGATCCGTGCAAAAGGAGCTGCTGAAGCGACTAAAGC

 

---

Search Again