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CRIMIC CR01196

Gene magu

CG2264

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:10059296..10059308	46D9	FBti0210326	86339

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CTTCTGGTGTTTAGTATCTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTGTCCACAAAGCGTAGGA	AAACACCAGAAGCTCGACAA	GGTGCATTCGGGGTTTCATT	ATCGCACTCTCCCTGTTTGG

 

Amplicon 5'

Amplicon 3'

TGTGTCCACAAAGCGTAGGAATTCGCATTTGATTTAAGAAGAGAAGTTACCGATTTCGTTCTTATTCCTATTTGACATAAAACAGGCATAATAGAACTGAAGAATTCGCATTTCAGGCATTTATATGCAGTCGTATCTTTCGTGCTACTACTAAAGCGTATTCCAGATTCGACCTAAAAGGCTAGCAATCCTTTCTCATTCTCATTTAAATACCTTTTTTAGTCATTTTGCCAATGTCACTAAGCTGCCTGATGATGACAAAGTGTGAAGAAGTATTTAAAGTGTGGAGCTTGTTCTTCTGATTCTTCTCCGCCCATTGCAGCCTCCTCTTCGCCGCCTCGTTCTTCGAGCGTGAGAACTGTCAGTCAATAATTTTGTCGTTATCTTGGTAACAATCCTTGAGCTGTCTCTGTTTATAATTTGTGGCTAAGAGGGTGAGACAATGTGAGGGGGGCGGGAGGCAACAGGGTGCTCCAAGTGGGAATTTTCAATCCAGGCACGTTTTCGGGATAACGCCCTATTTCAAAAACTTAGATAATCACCAGCAAAAGTGTCATTTGCATTCCCAATCAGACATTCCCCATAGCAGGGGCATTGCAAACGTGAAATTCGAGGGGATTTTTATAATAACATATAAATTATAAACATTTAACACACTTTTGCATGTACAACAAGTATTGTATGGAACTTAACAAGCTTTATAGGCATTTGCTCGCCAATATCCGAAATTGATAATTAGCTAATGGGGTATTCATGTTCAACTTTTAACCAGATGCCACACTCTCCACTTCTGAGAACAGCCATCCGATCTGCCTATTGCAGCAATATACCTATGTACATATGTATGCTCTGGTTATACATATATGTACATATGTACCTATGTACATACTTTTGTCCGATATCTGTCTGTGCCCAGTCGGGAGTCGAAAATCACCTGCGCACTCCGATTTCCAAGCAGCGCGTGCTTCGTTGCTCACAAAAAAGAATTCGAATTGTTTACAGACATTCTACATTATTTGTATACCTACATATATGTATGTTGGGGCAACAAGATAACACTGGGACAGGTAATATTTGTGCTTCTCATAATTGTATAACATTTCTTGTCGAGCTTCTGGTGTTT

GGTGCATTCGGGGTTTCATTTTGGGTGTCCACATAAACGCATTTAATTTACTAAGCCGGATTATAAAATGTTGATAGCCATAATCCCTTCTTGTTCATTTGATTCCCGTTGGATGTATGGGTTTTTGGGCTTTATTCATTGAAAGTATTCAAAATGATACAGAAATGATAGAGAAGTATACAACTCACACACTTTAACTACCATTAGCACCTCAAGTAGTATGCTCATATCTTATCCCAAACATGTTTCAGTTATGGGACATTTAGATGGCTTGCCGCATTTCACTTTTCAATAACAGCGAAATCTAATTAATGTCCGAGTGACACGACAAATATGTACATACATATACATATATCCGTGGGTCGGCCGGAGCAAATTGGCGACAAAAGGCGCTGCACTGGCAAATCCCAAAGTACTTGGCCGCCCCATCTGTGTATGTGTGTAGAAAGATATATAGATACAGATATTTGCGTATACTCGAATGTGATTTTGTGGGCTAAACAAGAATTTGCGCACTCAGCAGCCAAGTTGGTCGAGAGCAGCAGGCAGCCATAATTTTGGTGCGGATTTCGGGGCTTTCGAGTTATGGGCCTGACAACTTTGATTTTTCACATTAGCCGGCTAACAGGTAGGCCACCCCCAAAAAGCCCCCTTCCCCTTCTGCACACCACAGCCACCCCCCTTAGGCGGCCGCCCCTGTCGACCATTGGCCACATTCGTATTCCAGTCAGCGGCAAAGGCCCAGACGCTTGGCGACTCGCAGACTTGGCCATAAGTGCAGTAAATCATTTTACGCGTATTTGCCCAAGGGGGAGCGGTACGGGTAACTCACCCACTCCCTGGAGCTACCATACAGTTGAGACTTGCACTGACACCACCACCTCCACCACTTCTCCTCTCGATCTCTACCCGCAGATGAAGCTCCCCGCCTGCGCCGCCAAACAGGGAGAGTGCGAT

 

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