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CRIMIC CR01198

Gene CG14968

CG14968

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:3300202..3300212	63C5	FBti0202714	81214

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTTATTTCTCTCAGTGCCGGGCTGT	CAAAGGATAATTGCGGACGAGCGTTG

Images

Construct Design Sequences

sgRNA sequence	GATGTGGTATTATTCATCGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAACTCCATCGTGCAGAGGT	ATACCACATCGTCCATGAACA	GTGGCATTGGTTTCCGCTG	TCTGTTTACCTGCGCTCGAG

 

Amplicon 5'

Amplicon 3'

CAACTCCATCGTGCAGAGGTTTGAGTGTCCTGTGTTTTGCCTAATCGCATTCAATTTCTGAACGGTAGAGATGGTACGCTTAGAAACAACCTAAAACAATTTTATCTTATAGTTGAACTTAAAATAAAGTAACAATATTTAAAAATAATTCTATAATCCTTATTTTTTTTCACCACTTGTAATATATGTGCTTAAAAATCAAAAGACTTAGGACACAAACTGCAAGTGGCATATCAACAGGTTGTAGCCCAATTCAAATAGCCAAACATACCATATTCGTAATTTATTTTAGAAAATCGTTTACAAATCGTATTAACACTTAGGCTTTGGGTAATTAGCTGTATGAGGCAACTACCAGGTGAGAGAACCACAATTTACAGATACAGTGAACACTGGACGTTAAGTAACTAACACATCACATCTCATTGACGGAGCCAACGCAGGATCACCAGGATAACTACGCCTCCGTTTCCTGCTCCAGTTCGCCAGCCTGGATTTCCTCCGGCGTTCGCAGATCGATGGCGAACTTGTGTCGCGTGGGTGTAATAGTGTCGCTGGGCGTGGCCTCGCCCTCCTCCTCACCGGTTGCCTCCTTGACCCGTCGCGAGTAATCCTGGAAGGCGGCGGCCAGGGCAAAGGTCAACTTCCGCGCCATCGCACGACTGTCGCACACGAAGGCGTGAACCTCAAAGGGATGCGGACTCGACTCGTCCTTCACCACGATCATGGCAAAGACCCTTGTGTAGACCAGGTCCTGAACGCCATACGAGATCGTGTCGATGGGATAGCTCCAGTGATTGATGCTGGCCTTTGGCGATATGATCTCCAGCTGGACTCCGTCGGTGGACACCTTCAGTTCGCAGTTGGGCAGCACCTTATTGGGCGGCAGGTTCTTGGCCACGCCCACCATTATGTCCACCGGACGACGCGTATACTTAATGCCCCATAAGCCACGTGCCACTTCGGAACCAATGTACTTCACCTGCAGGGAGAAAATGTATACGATATATTAAAAAAAAAAGATTGCCGATTCTACACTGCATTTAGTCAAAAAAAAAAAAAATTCCAATGAGTATATAACCAAATATTCCTATTTTACGTGAGTAAACAAATATATGTTCATGGACGATGTGGTAT

GTGGCATTGGTTTCCGCTGCGCATGCGTTGCCGTTAGAGTCGCTTCATTTGCATCCGACCTGCGACCGGATGTCCACAGCCAAGCCAGAAAGCTGTTGCTGTTGCTCATGTGGTGCCACTGCCACGCCCCTTTCCCCTCACCGCCCCCAAAGTGAAGTTCAGCAGGGCGTGACGCATGCGTGTCTGGCTCTTTTTTTTTGGTCATGTCTCATGTGCGGGTGGTATTATGTTCAGATGTCTGCTTTTGTTCGTTTATGCACTCATAAAAAAAGAGTTCATCGAGAAATTAGGATAGACATTTTAACAGTGGATTTTGTCATATGTATAAGTAGCCAATATGGAACCAATATACTCATTTCATTTTATCATTAATTTTCAGTGTACCTAATTTTTTTCCGTGTATTTTCGGTCATATACGTAAGTGGTCTTAATGGGGATTGGTACCTGAACTTGCGCCACATATTTCACAGCATTTAAATTTGAAAACGCATTTCGCCATGTGAACACTCACCTTGAACGTTATGGGCAAATCCTCCACATTGACCTGGCTGTCCAGATCGGTGGTACTACTGTTGTTGGAGGCCATTCTGTAGCTGTATCTGTATCCGTATGTCGTCTATCGCGAAAGGTGCAAGCGAAGATCCACCGTTGAACGCATTCACCGCAAACCAACTGAAAGGGCGCAAAAAACACGAGCTTGAGTAATGCTCCCATTAAATGTATCTCGCGGATATTTTATGCGCCATCTTACGGAGTTCACATCCACCGTTGCATTACCATATCTCTACCGTAATCGCTGAATTTTACATGGTTTACTTTGTGTGCGCTTTTTGAACCTATATATGTTATATTCCGCTGAATATCGGTCGGGTCGACTAATGGTTCTGTTTGGGCTTGTTGTTGCTGTCGCCGGCTACGGATTGCTACTGAACTAGCGATCGGCGGCAAAAGGTGTCGCAAGTTGTGCCCCCAGGTAGCTCCACTCCCTCTCACTCGCTCCCAATGCCACCTGGTCCCCCTCCCCTCCCCTCCCTCCCACTCGAGCGCAGGTAAACAGA

 

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