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CRIMIC CR01203

Gene Hcs

CG14670

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:5476153..5476158	83A5	FBti0210327	86340

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	GGGTGACGCCGCAATGTGGACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGAGCCAATTACGAAACCGC	CACATTGCGGCGTCACCC	GTGTTGCGTGCGGTAATCTT	TGGACAGCCGCTAGTTTCTC

 

Amplicon 5'

Amplicon 3'

GGAGCCAATTACGAAACCGCCATTGATTTTGGTTCCCATCAATATGTAAGTTGTGCCCCGGCTTCTGAATTAGTCCCCAAAAAACTTTGAGTCCGCGGAGGAAGCAGTACCCACCAGAAATCTGCGATGTTGACCCTGTATTACGTGAGTGCCACCGTCCTGCAGTCCTGGCGCATTCAAAAGGCGTGCTCCAAGATCGCCGAGCACCTCGCGCAGCCCTCGAGCATCGCCTTCTATACCCTCCAATCGGGCAGCGATGACGGATTCGGTAAGTGGCGGCGTCATTGGAAGGCGTTCACGCTTATATAGACTGCCTGCTTCCTCCACAGATCCCGCCCTGGCCAGCTCGGAGTTGTGCTGCAATCGGAACGCGGCCAAGGTCACGGACATCCTGTGGCTGCACGCCAACCAGCGGGGTTGCTGCCTGCGTCCGCTGCAAACGCTACACATCACGCCTTGGATCAGCTTTCCGCCGGCGCCCAGTCTGCTTCCCTTCTCCTATGCCGCCGACACGTTAACGCCGGCGTCCACGCCCACGGAGGCGGACGTCCCGCGCCAACAACGGGTTTCGCTCTCTGCCCAAGGGGAGGAGCGGATGCAGCTGCTCCTTGAGGCCGACATAGAGCCACTACAGCGGCCCAGCTCCGAGGACACATCAGCAGTACGGGTAAGTTAACCCGACGGCGTGTTGCGTTTTTTTTAATCTCCAAATCCATCAATAAAAAACAGTAAGGCTTAAGCAAATTCAAGTTATAAATGTTTAATTGCCACAATTCGGAGTCCACAAATACATGTTTATACATGTTTATTTGCAACAGAATAGTTCAAAGACTTCAGGATGTAGGAATAGCTTTTTAATGGACTTTTAACTACATAGTTTGGAATTCACCATTAAAGGGGGACTACGAATATTACTCCAAAGTATCTTAAGAATGCTACCGTAATTAAAGTTAGCCCACTTTGTATCATCATAATGAGAACAGATAGGGACAATCGACCTTCGGGTGACGCCGCAATGTG

GTGTTGCGTGCGGTAATCTTATTAAGATGTACATGGTTGAATCGATTAGAGCGCTGGCGTCATACATACATACATACGACTACACTAACTAGGGGTATCCTCTCGTGTGTCCTTTCTTCTTCTTGCATGTCGGCGGCCCATGAATCAGCTGGAAGATTACGGTAAGCTAATTGCTTGGAAAATAGATTCCCACCTGGCGGTGCTCATCGAAACAGACGTCGAGCACTTCACGAAACTTTTGATAACGACTTTCTTGAGAAATAATTTATGCATTAACGATCAGCTGCCACTTTTGCGGATTGAGTGTAAGCTGCACCTGAAATCGTTTTAAGAATTTGACTTCATCTTAAAAATCCCACTGATTTATTGTTTCTCCCTCCCAGCGGTGCAACGGGAAGGGGATCCGCAGCCCTTTGAGCTTCTGGCCAAGCATCTGAAGCGGCAATCCCGCATAAGCGTCGGTCTGGACAAGGATGGATGGAAGAAGCACATGGAGGACTTGCGTGCCGTAGGTGTGCTGGCGCACCAGGCCACCGAGTTCGAGTACCAGAGGAATCGCTCTGAGGGAAGAACGAAATCGGATCCCACCACACATGATCAACGCCCGACTTCGGAGCTTTTGGCCAAGGCTGTGGCCGTGGTGGAGCCCACCAACAAGGCATATCTGTCTCCGGCAAGGACGACAGAAGCCTCGCTCAAAGCCGAGGCCAAGGGAACGTCAACAAAACCTTTTCCGACTAAAAGCGATGCCAAACCCGCCACTCTTGCCAAGAGCGAGGGCACGCCAGCCACAAGCAAAGAGGACTCCAAGTTAACGCCAACTAAGGGTGCATTAAAGACAAGCGAACTAGAGAAACTAGCGGCTGTCCA

 

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