• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01298

Gene CG8837

CG8837

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:3321106..3321123	23E3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TACCTGGTTTGCTGGCATATGAGGAG	AATAGAGCCAATGCAATGACTGCCGC

Images

Construct Design Sequences

sgRNA sequence	ATTCAACGAACCATGTGTGTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCAAGATCGCTGGTATCGT	AATACTGACGTCCGCCTACC	CACATGGTTCGTTGAATCGGA	ATTGCACACCAACCTTTCGC

 

Amplicon 5'

Amplicon 3'

GCCAAGATCGCTGGTATCGTTATCGATATACTGTTACAGCATGTTTCCACTTGTTTGCAGTACTTCCAATCGTTAATCAAATTGTAAGCAAATCGTTATCACATGTATTGTGGTTATCGAGTTCAAAATTAAAATAAACTTTAAGATTATTTATTTTGTTATTTATGCTCGCTTTGTATATTCATTTTTATATTTTTTAGCTTCTTTATGCTTTTTCTCTTTAGCCTTTCAGTTAAATTGTTTCTTCCAATTAATGTTCAATTGCCTTCAATTGGTTCGCTTCTTTTTAATTAAAAAGCATTGTGTGCCACCCTCAGATTTCACTATAAACAAAACTAGCTGCCACGGCAGACGTCCTCAGATCTTATCGCCAAATCGTTCTGTATGCTCACGTTGGGCAAATATATATCTGCATATTGCTCATCCCGGCCATCTCAACTACACGAAAACACGTCCGGACACAGTCTACGGCCGTTTCGTGGCCGGTGGATAGGGTTCAACTGGTGGCGCATAGTAGCGTTGTTATCTCTGGACGAGGGTTAGATAAAGGCACCGATGCCATGCCCATAAATACCCCACAGCATCCATTAACTGATTCTCATTTCAGCAGTCAGTGCAGTGAATTGCAGGTGTTTTCCCCCAGGTTTCCTCATTGGATTTTGGATACAGAAGCTATATAAAAATGTGCCTGCCCAAAAGAGTGGGTGGTCCGGCCATCCAGAGTGTTGCCACGGCTTTAGGTGAGCTTCACAGGATTGACTCTTGCTTTAAGAGAAAGAAGTAGTTTCAGCACAAATTGCATACTAATATTAGTATTAGTTGGCCACTAGATGTGCGCTCTACTCTCTCTGTACGTAATAAAATGCTCCAATGTTTTTTTTTTTTACCCCATTCCAGGCAACATTCTCTGCTTTAACTTTGGCCTCATGTTTGGGATTACTCCGGCGCACATGACGCTTTACGAGTCGGAGGAGAGGACCCCGTTGAACCAGGCCACCGATCCGGCGGGAACTGCATGGCTGACGGGATACCTCTTCCTCAGTGCCGCCTTGGGAGCCCTGGTCTCCGGATTCCTGGCCCTGAAAATCGGGCCCAAGTCAGTGCTCCTCTGCTCTGGCCTGCTCCAAATTGTGAGTAAAATGTGAAACTAGAATGGGAAGGGGGCATGTGAAGGCCAGGGGTAGGCGGACGTCAGTATT

CACATGGTTCGTTGAATCGGAAAATTTGCATGTATATCGACATTTTTTTGTACAATGTGTGGAATTTAAATTAACAGTGTGTCGCATTTTTTGAAGTGGAAGAGAACACTGTTAGAAAAATTTTATAGTGAATTGTAAATATCTCCATATATTTAAAAGACGCATGACCTAGTACAAGACATATTAAATTGCCCCATTAAATTCACGAATATATTCCAATACAGGAAGAAACTTATAGTTGTGATGTCTAAACATCCAAGTCAAAGCATAAATTTAAATTTAAATTCATGTGAAAGCCAGTAAAATTATTTATCTTACTAAGTATTTAAATCAGTGAACGGAAAATATTCGACTTTTTCATGGAAATAAAACTGAATACCACAACTCCTTCCAGTCGGGTTGGGCCTGCATCCATTTTGGCTACGACATCGTGCACATCTACGCCTCCCGTTTGTTTGCGGGCGTGGCATCCGGTGCCGCTTTTGTAGTGCTTCCGATTTTCATCAACGAAATTGCCGAGTCGCGCGAAAAGGCAGCCCGCCTAACATTCACCATTGAGCTGTGGCGCACGCTGGGCATCTTGATTGGATTTGTCCTGGGCTTCTATGTGCCCTACGCCTTCGTGAACATCGTCGGCTGTGCGGTGTCCTTTGTGTTCACCATGACGTTCCCCTTCGTCCAAGAGAGCCCCCACTACTATCTGCGCAAGAACAACATGGCCAGCTTGGAGAAGTCGCTGCGCTGGTATCGCGGCATCCGCGACATCGATGATCGCGAGAAGCCGGAGTATCTCAGCGAGCTGAACGAGTTCCACGCGGAACTGAGGAGCAGGGACAAGAACGTGGGCTCCACGCCAATGTCGCATGGCTATATCATACGGCTGACTTTCGTCAGCTTCCTACTGACCGTGTGCGCCAAACTGAGCGGCGTGTTCGTGGAGCTCAACTACGCTGCCGACTTTCTCGGCCGCACCGGCTATTCCACGGAAACCAATTACGTGGTTTTGGCCAGCGCCCAGTGCGCCGGAGCGCTCCTCGCGCGGCTCGTTGGTCCGCGTTTGCCGCGAAAGGTTGGTGTGCAAT

 

---

Search Again