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CRIMIC CR01300

Gene CG9527

CG9527

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:6447493..6447519	26D1	FBti0210328	86341

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CGGGCGACGATTAGACCGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTGGTGTAGTTTCCCTGCG	TCGCCCGTTTCTTATCTGCA	TGCAGCTCCGACAAAAGCTA	TCAAATTACGCGCCAGACCT

 

Amplicon 5'

Amplicon 3'

ACTGGTGTAGTTTCCCTGCGCATCGATGTCACCGGTCTTGGACAGCAGATTTGCCTTAGGGATACGATACTGGTTGAACATCACAAATCTGCAATGCCAAACAAATCGAAATTATTTCTGTTGCTGTTGATTAGTTTTATCAACTGATAGAAGAAAGTACTTGCCCGTTATCAATGCCATTGAGACCGATCTTTTCGCCCATATCGCCGACGGTTACGCCGGGGAATGGAAGCAATGTACGCTCGTCCCGAATGGGAACAAGGAAGGCCTGCAGGCCCTGGTGCTTGTCATCCGGCACGTACAGCTGGGCGTAGACGATTGCGTGCGTGCAAGTCTTGCCTAGATTACCGACCCAACACTTGGCCGCCTCGAAGTCTGGCGTGTGGATGATAAACTCCTGCCTTTTCACATCGTAGGTGGCACGGGTGCGCATGCCGAGCGCGTTGGTGCCGTGAGAAATCTCAGTGAGAGCGTAGGCGCCCAAAATGCGGTTGTCCGCGATCTTGGCCACGTACTTGCCCAGGCGGCCAGATCCGTTGGAGACCAGCGTGCTGGGGAACATGCCAGTTGAAAGGCCGAACTTGACCGAAGTGCTGAAATCGTAGCTAAAGATGGCCTGTCCGAAAGCAAGCAGAAGATGTGGGGTTCCAAGATACTGAAAGAAAGACAGGAGATTTAGGCAGCCACACTTTCAGGAGTACGCAACGCATCCTACCTCGTTGACGCCGTAGAACTGTTGCTCCCACAGAAGATGCTGCCGCTTGTTGGCCATTTCGCGGGTGCGTTCCAAATCGGATCCTGGCTCCCGCTGGTAGTCGGGGTGCTGTTCCATCCATTGCCACACCTTGTGCTAAAGACGGAGTGACATGTAAACGAATTACTCAAGTGTGCAGATAAGAAACGGGCGA

TGCAGCTCCGACAAAAGCTATCGAGGCAGTCTGCACTTCGACCCCGCTGACCATGTACATATGTAACAAACTGGAAATGGTAACGAACCTTCAGCCGTATGTGATCCTCGCCCTCGAGCACCACGTTCATGCGCTTGTAGCAGAAGCTAGCCCGCTCACGGTACTCTTCCAAAGGTCCGCTACGGAAGTCGGGAAAGATCCGTTTGTCATCGTAGATGTTGGCTGCAGCCTTCTCCTCCCGGCGGCCGCTGTAATAGTCGCCCAGTGCGGTTAGTAATGAGTTCAGACCGTTTTGGTCACCACCATCGCCATCGCCTACTCCGCTCGCCATTGCCCAGAGAACTGGCTCTTGTTGGAATGTTGGATTGGTGGACGTTGTGGTCGTCAGTCGAGGCGATGGGGCTGTGGGGCAGGGGTACTTACTTTGTGCTCAATGCAACCATGTTGGAGTTCGATTCTAGCGACTGGTCTGGCTGCTTCGAATGCTAGGCGCGAAATGCTTGAAAGTCCAGGCACTCGTAACTATTATTGGTAAAATAGCCAATGTCCAATGGCCAGCGGCGATAAGAAGGAATCCGCTTATCCGCACTTCATGGCCAAGTAGCCTTTTAGTGGTTTGCATCCGCCGCGGATAAGATACGGGCCCTAGGAGAATGCGGGATTAGGGCGGCCCGTGCAGGTGGTGGTGGGTGCTGCCTGCGAAATTCGAATCTTTTAGCGGCCACAAACATTGCTTTAATTTCCTATCAGCCATCAGCCAACAACAAGCGCGATAAACAAACAATTTTAACCCAGTTTTTCACCATTGAAGATAACATAGTTTGACGTGACTTAAGCCATGAGAAAGAACGGTGTTATATAAATACAAAATGTGAAGTTTTTTAAATTCTGTTAATTATTATATAATATAATTATTTAATTATTATACATAATATACATAAATTTCATGTTTAATTTTCTTTAATATCACAATTCAATGTTTTCACAAAATGTTTTAGCGGGGGCGTTGCCAGACCTTCTCAAGGTAGCCAGGGCTGGAATAGTAGGTCTGGCGCGTAATTTGA

 

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