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CRIMIC CR01302

Gene vkg

CG16858

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:5025521..5025546	25C1	FBti0206886	83258

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	GATGGCAAACACGTGAGCGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCCTCACCGATCACTTGAG	AAGTGTGCAGCTCGTCTGAC	GGGCCAGATTTCCAGATCCC	CGGGGGTTCAATGAAGTCCA

 

Amplicon 5'

Amplicon 3'

CCCCTCACCGATCACTTGAGCTGCTGCTAATTGTCGGAATCGAACTGCGCACACACACACAAGCGCACTGGCGAAAGAGCACACTTATTCACAGGCAATAACGGCATTTCCATATATGTATTTATAGCTGGGCAAGTGCAGGAGAAAAGAGAAAGGGTGCATGGGGTAGTGGTGCAGTGGTAAAGTGGGGAGAGCGACTGATAAAGACCATTTATCAAAATTCATTCATTGAAAGCCTCAGCTGCATCTGCAATCGATGAGTTCATCTCTCTCTGTCTCTTCTGCTCTTCGATTGAAGCGGCTCTTAAGCGATCGGCTCTCTTTTCGCGGAACGGAAAAGGAAATCATTTGTTCGGCTAATGAAGCGCCAAACTGGTTGCAAAACCCATTGCGAGAACAGTTTTAGAGCCATTGCCTGAGAGCTGTTAACTGTTTAATAAGTATATTCAAATGATGCTGGAATAAATACCCTTAAAAACATTAAGTATGAATATATCTTACTCATAGCAGATAATTCCCTTTATAAATTTCTTTATAGATTGTAAATAATGGCATACATACAATGTGTGTTTCTAATCAAAACTAATTCCAGCGAAAAATGTGAATCCCCTTCTATTCAGATTAGCTTAATGCACCCAGGATATAATTAATTATATCGTTTTCAAATACTGTTGCAAGTGAAACAACTTTTTTTGTAAGCCTTCGCTTATCGTGAGGGTAACACGAGCAACGGTAGCTAAGCGAAGTCAAGTTTTTCATCGTTTTGCGGTTAGTCCTCCATTGGCAACCCACAAGACCTGGAGACCTGGCAGCTCTCACTCGGAATCCGGGAGTTAGGGTCCCAAGCCCATGGCATTGACCACGTAACCGAGTGCGAAAAGAGCTGTGGATCGGATTACCAAAGTCGTCAGACGAGCTGCACACTT

GGGCCAGATTTCCAGATCCCAACGAATTGAGTGCACGAATTGTGCATTGAGTTAACTGCTCAACTGGTATGAAAACTAAACTCTGATCTCTGGTAAAGGCAATAAAGTCAAGCCAAGCCGATAGCCTCTGCGACTGCTGGCGACACTTGTTTCAATTCTAAAGTGTATTTATTCTAAATTCGAGTCTAGAAAGCCATGTCGCAATCAGCGGAACATGCTATCAATGGCGTCAATGCCGCCAACATAGCAATAGGTCTAATTAAATGTGTGTAGATCAGTCGCAGCCTGATAAGACCAGCCGAATGCCATCAAAAAGTAGGTGCCGCCGGCATGTGCTCCATTTTTCATCCAGCATTACGAAATCTCTGTGATCCCTAGCCATTTAAATATTAATACTCAATTTAATTTGGTACATACACAAGGTACACTGAATTGGGCAACTCGGTGTTAAAAAGTAGTGCATAGTTTTTGGGGCAACATTGAGTATTGCGTTTGCCTCGCACTTTTGTGTCAAATCATTTTTGTGTTTGCTTAAAAAATCATTCATAAATAAATGAAATATTCGCAACCTCAATGGTTAATGTGTCTTATTTTGAATAATAATCTATTCAATTGGGGTAATAACTAAGAACCTGCGCATTCTTTGTTTTTCATACTCATTCCCGTTTCATTTTACAAATGATTATATGCCAATTAAGCGACTAATCTGGCGGTTATTAAATATAATTCTAATTGCCAGCCGGAAACGTTCAATGGCGAACCACAATTGGTATGCCAAAAAAGCAATGTCCTCCAAATTGAAAGATTAGGGGGGTTATCAATGGGCGTTATCGCCAACCTCTTCACCTACAAAATTATGTTGCCAAAATTATTTAATGACATAATCGATCCCAAGCTGGACTTGGACTTCATTGAACCCCCG

 

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