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CRIMIC CR01303

Gene Eaat1

CG3747

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9339155..9339164	30A8	FBti0210329	86342

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CGGTCCTATCAACACGGTTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAAGGCCTTCATGCAGGAG	CCGTGTTGATAGGACCGCTT	GCCCCAGACGCAAATTGAAT	CATAGAGAGCCGTTCCGTCC

 

Amplicon 5'

Amplicon 3'

TCAAGGCCTTCATGCAGGAGAATGTCCTTACCATGGCCACCGTTATCGGTGTGTTTGTGGGTGGACTCATCGGCTTCATCATCAAAAATAGCACTGGCGAGTGGTCGAAGAGAGAGATCATGTACATATCCTTCCCCGGCGAGATTTTCTTGCGGTGAGTAAAAAGAAGTGGGATAAATTCATCTTAGTTGTATCAGATTACTGAATTGGAATACTATAATAAATAACATATATTTTTAGAATGCTTAAATGTTTGATTGTGCCGCTTTTGGTCTCATCAATCACCAGTGCCATTGGTGGACTCGACCTGAGCATGTCCAGCAAGATTGCTACCAGGTGAGTTGATAAGAAAACCATATGAAGTTAGATAGCCCAGATAAAATGGAACTTAATTCCCACCAGAGCCATTACTTACTACTTTGTGACCACCATATCGGCCGTGATTCTGGGAATATGTCTGGTGACCACACTGCGTCCCGGCCAGGGAGCCAAGATCGTGGAGACCCAGACGGAGAGCATTGATAAGGCATCGAAGGTGCTCACCCCAGACACGCTTATGGATTTGGTGCGGTATGTTGGGATCTGCCGGTAACTGAACCCGCCTTCTGACTCATTTTATTTATACCCAATAGAAACATGTTCACGGACAACATCATTCAGTCGACCATGTTCCAGGTAAGACTCTGTTCCAAGGCGAATCGTTCATACGCACTGTTGAACGCTAAAAGCAACGGTAGCAATTATTCAATTCTTTGCGGTTTGGCGAGATTTTGATTGAAACAAAGCGCGACACATAATCAAAACTGACATGATAGGGATACCACAATTTCAATTACAATCCAGCTTCAAGTAGGGGGATTTCACACAAGTGCCTAGCGGTGTGAGTGAGTGTCAGTGCTTAAATTTCAATTATCGCATGTGCGACTGCGAATCGGGAGCGAGCGAGAGGGAAGCGGTCCTATCAACACGG

GCCCCAGACGCAAATTGAATCAAAGTACTTAGCTCGGCCTGCGGCAGATATGTGGACATTGTACCTCCATAATTATATGCATATGCGAGTGGATGTTGGTGTAGGTAGGCAACCGAATGAATATTCATGCGCCCAATTAGATTATTGTTGACTCAATGAAGGAAAATAACAGAAAAACCACAATTTCGCTTATTCAGATTCTACCATTTTATAAAACAGATAACGAAAAAGTCTGCCAAATCGCAACGGATGATGAATGATGGGACGATTATTTCTCGAACTACTGCTTTTGATATGATTAAAATCCATATTATTGTATATATAATCTATTTACACCTGCTCCATTTTAGCACCGCACTGAGATCTATGAGAACACTAGCATTAGCCCAGCACGTAAGTTCTTACATTGGCATCATAAACTCATCGTGAAACTTATTCCTATAATCCCCTACATAGAGCCTATGGAAAACTGGGAGTTCAAGTCGGCTCAGCGCGAGGGTTCTAATGTCCTGGGTCTTGTGATGTTCAGTGTTATCCTAGGTACCACCATTGGAAGAATGCGGGAGAAGGGACAACTGCTGCAGGATTTCTTCACCACACTGAGCGAGGCAATGATGACCATCACCTCATGGGTTATTTGGTAACGCGAGATGTAATTACATATCACCAAGAATTTTAGGAAACAATTATTGATTTCTTTAGGATTTCCCCGCTGGGTGTTGCCTTTCTGATAGCCGCCAAGATCATTGAGATGGAATCGATAGCAGCAACGATTCAGTCATTAGGATGGTATTTCATAACGGTCATGATAGGTCTATTCCTTCACGGTTTTGGTAAGTTATGAACACCGTATACAATGATTTAAATTAACAATTGGGTTCTATTTTAATTTCAGGTACGATTGCGGTGATCTTCTTCCTGGGCACCCGACGTCTCCCGTACCGCTATATTGCCAAGCTTAGTCAGGTCCTGGCAACTGCATTTGGAACAGGTTCCAGCTCGGCCACCATGCCGCTGACCATCAAGTGTTTGGACAACATGGGCATCGATCCGCGGGTTACTCGATTTGTCATTCCTGTTGGTGCCACAATTAACATGGACGGAACGGCTCTCTATG

 

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