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CRIMIC CR01304

Gene CG31712

CG31712

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9937577..9937601	30F1	FBti0202715	81215

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGTGCAAGTCCGCAAGCATACGTTAA	TGGCGTAGGAAACTCACCCGTTATCA

Images

Construct Design Sequences

sgRNA sequence	CGCAGCATGAGTGTCCATCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACACCATACCACTTCGCTGG	CACTCATGCTGCGCTTATGA	TGGAATGCGTACCCATGGAG	CTTCCTACGGCTCCTTCCAC

 

Amplicon 5'

Amplicon 3'

ACACCATACCACTTCGCTGGGCTTTGGTATTTCTTTGATTCTTTTGATATTTATTGTTTGGTGTGGAGGAAATGCGTCCGTGGACCAATCGCTCGGCAGCAGGCTTGCTCAACTTCCGGCATGGCGGCACAACGTGGAGTGCACGCGAGTGTTTTGCGGGTAAGGATCACAAAACAGCCACACACACCCACTTGCATACGGGACAAGAGCAGCACGTTCGTTGCACGGGCCTACTGAAGCGGAATTTGCGCGCAATAACAACAACAACGAGTTACGAGAACCAATACCAAAAAAATTAAAATGATACAGTATGTGGGTAGGAACGAACAAAAGATGAAGCTAAGAGTATGCAATAAGTCTGCAATAATGGGCATCAAAACTTTGACACATGCGACACTTGGCAATGTCGTCATTTTGGCTTTTTTGGTAGGAAAAATAAAAAGTTACAAAATTATTGGCAAGAAGAAAAAAAGGGGCAAAACGAGCCGTGGCGTTCTCATCTCGGCTCACAGCATTTAGCTTGCTGCATCATGTGGGGAGGGCTCTGAAAAGTTGCGACTGGTTAGTGATGTACCCGGTTAGTGGGCGCCCTTGGCCCAGGGTTATGCAAGCAATCTAGTACGGGGAGGCTGCTGATGGGACATGGGTTGATGGATGAACAGCCAGTGAGCCGCGACTGAGAGCGCCGCCGGATCGATTTCGCGTGACGTGGTGGAGACGACTGTTTGAACTGCTGTTTTGGATTTATTCAAGTGTACGGTAAATGTGGCATTGTGTACGTGTTTCTGACAGTGATAGCAGCAGTCGTCCAGCGGAATCTGAATACGAGCACCTACCTCGCGCCGGCGTTCTTCTTCGCGTATCTGCTCTCGCCGCCGCTCCAGCTCCAGCATCATTTCGCGCTCCATCTCAGCCTTGGCGGTCTCAACGCGTCGATTGACCTCCTGTTCGATTTCGTCGCGACGCTTTTCTAGCTCCTCCTCGACGCGCTTTTTTACCAGCATCTCTATGCGCTTGGCGGCTTCCTCTTCGATTGTCTTTTGCTCGAGCTCCTTCGCCCGACGCTGCCGCTCCATCTCGGCCAGGCGTTCGACCTGCATCTGGTTTTGCTGTAATGGTAGATCATAGTTTAGTTGCTTTCATCGAAACAGTTGTATCATAAGCGCAGCATGAGTG

TGGAATGCGTACCCATGGAGTGCTCAAGATGACAGACTATGGACACTATCCACTTACCTCATCCAGCTTCTTAAAGCGGCTGCGCTTCTGTTTGCTACGTTGCGACTCCTGCTCGGAGTACTGACTGTCGCTGCTCGACGAGCTGGAGCGTTTTCGGGATTTCAGGATCGAGGGGGAGTGACGGTAGTCGCGCTCAGTGTGTCGGTCGCTGCGGTGTCTATCCCGCTCGCGGTCCCTGTCCCGTTCGCGATGTCGTCCATGGTTGTTCACCTCCGAGGACTTGTCTCTATCCGTTCTTGTGCTGGGCCGCTCGTGGTCGTGGTGGGACTTACTGCGTTTTTTGTGCTTGCTCTTATGGTGGCGTCGCTTGCCCGACGGACTGGGTGTACGTGAACGGGAGCCCATGGTGCTGGATTGTGTGATTGTATAAGATTTCGAGCTAGTCGAAGGGCTTAACTTGGCTGCCACAACAAATCAATGAGAGCGAATTTAATTTCTCGAGCAATTTTCACAGATGCCTTAAAACAGTGTGCACAACGTTGGCATACTGGCCTGACATAATTCTGGTATTTTTTTTGATACGGTATTTTCTGTGCTTTTTTTATTTTAATTTCTGTGTTGCCCAACATTAGACCGGCAATTGTTTTCAGTTCACATTGGTTTGTTTTGATCTAAAATCATTTGGTGGAACTTGTCGATCATGACTTGGTTATCCTTAAAGCCGACGCACTGCTTGGCCTCCTCCTTGGGCAGCCACTTGAGGTCGGTGTGCTCCTCGGAGAGAATGGGCTCCTGGCAGGGATTCCGCAGCTCCGCTAGCCAGTAAATAACAATCTTCGGCTTGTCTTGCACCTGATAATTCAGGGTCAGCGGAGTGTCCTTGTATATGATCAGATCCTTCTCGTCGTACCTATGAGAGCATTTTTGAAATTAAGCATGTTTTAAAAGATATATCGTTGTGCACTTTCCCGGCTTCCTCTTTCGTTTCTCGCAGGGCAGTGGTAAAATCATCCTCGCCTGGGTCCACATGTCCCTTGGGAGAACTCCAGTGGAAGGAGCCGTAGGAAG

 

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