CR01304

CRIMIC CR01304

Gene CG31712

CG31712

Information

Donor Location Cytology FBti Stock number
pM37-p02L:9937577..993760130F1FBti020271581215

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence CGCAGCATGAGTGTCCATCCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACACCATACCACTTCGCTGG CACTCATGCTGCGCTTATGA TGGAATGCGTACCCATGGAG CTTCCTACGGCTCCTTCCAC
 
Amplicon 5' Amplicon 3'
ACACCATACCACTTCGCTGGGCTTTGGTATTTCTTTGATTCTTTTGATATTTATTGTTTGGTGTGGAGGAAATGCGTCCGTGGACCAATCGCTCGGCAGCAGGCTTGCTCAACTTCCGGCATGGCGGCACAACGTGGAGTGCACGCGAGTGTTTTGCGGGTAAGGATCACAAAACAGCCACACACACCCACTTGCATACGGGACAAGAGCAGCACGTTCGTTGCACGGGCCTACTGAAGCGGAATTTGCGCGCAATAACAACAACAACGAGTTACGAGAACCAATACCAAAAAAATTAAAATGATACAGTATGTGGGTAGGAACGAACAAAAGATGAAGCTAAGAGTATGCAATAAGTCTGCAATAATGGGCATCAAAACTTTGACACATGCGACACTTGGCAATGTCGTCATTTTGGCTTTTTTGGTAGGAAAAATAAAAAGTTACAAAATTATTGGCAAGAAGAAAAAAAGGGGCAAAACGAGCCGTGGCGTTCTCATCTCGGCTCACAGCATTTAGCTTGCTGCATCATGTGGGGAGGGCTCTGAAAAGTTGCGACTGGTTAGTGATGTACCCGGTTAGTGGGCGCCCTTGGCCCAGGGTTATGCAAGCAATCTAGTACGGGGAGGCTGCTGATGGGACATGGGTTGATGGATGAACAGCCAGTGAGCCGCGACTGAGAGCGCCGCCGGATCGATTTCGCGTGACGTGGTGGAGACGACTGTTTGAACTGCTGTTTTGGATTTATTCAAGTGTACGGTAAATGTGGCATTGTGTACGTGTTTCTGACAGTGATAGCAGCAGTCGTCCAGCGGAATCTGAATACGAGCACCTACCTCGCGCCGGCGTTCTTCTTCGCGTATCTGCTCTCGCCGCCGCTCCAGCTCCAGCATCATTTCGCGCTCCATCTCAGCCTTGGCGGTCTCAACGCGTCGATTGACCTCCTGTTCGATTTCGTCGCGACGCTTTTCTAGCTCCTCCTCGACGCGCTTTTTTACCAGCATCTCTATGCGCTTGGCGGCTTCCTCTTCGATTGTCTTTTGCTCGAGCTCCTTCGCCCGACGCTGCCGCTCCATCTCGGCCAGGCGTTCGACCTGCATCTGGTTTTGCTGTAATGGTAGATCATAGTTTAGTTGCTTTCATCGAAACAGTTGTATCATAAGCGCAGCATGAGTG TGGAATGCGTACCCATGGAGTGCTCAAGATGACAGACTATGGACACTATCCACTTACCTCATCCAGCTTCTTAAAGCGGCTGCGCTTCTGTTTGCTACGTTGCGACTCCTGCTCGGAGTACTGACTGTCGCTGCTCGACGAGCTGGAGCGTTTTCGGGATTTCAGGATCGAGGGGGAGTGACGGTAGTCGCGCTCAGTGTGTCGGTCGCTGCGGTGTCTATCCCGCTCGCGGTCCCTGTCCCGTTCGCGATGTCGTCCATGGTTGTTCACCTCCGAGGACTTGTCTCTATCCGTTCTTGTGCTGGGCCGCTCGTGGTCGTGGTGGGACTTACTGCGTTTTTTGTGCTTGCTCTTATGGTGGCGTCGCTTGCCCGACGGACTGGGTGTACGTGAACGGGAGCCCATGGTGCTGGATTGTGTGATTGTATAAGATTTCGAGCTAGTCGAAGGGCTTAACTTGGCTGCCACAACAAATCAATGAGAGCGAATTTAATTTCTCGAGCAATTTTCACAGATGCCTTAAAACAGTGTGCACAACGTTGGCATACTGGCCTGACATAATTCTGGTATTTTTTTTGATACGGTATTTTCTGTGCTTTTTTTATTTTAATTTCTGTGTTGCCCAACATTAGACCGGCAATTGTTTTCAGTTCACATTGGTTTGTTTTGATCTAAAATCATTTGGTGGAACTTGTCGATCATGACTTGGTTATCCTTAAAGCCGACGCACTGCTTGGCCTCCTCCTTGGGCAGCCACTTGAGGTCGGTGTGCTCCTCGGAGAGAATGGGCTCCTGGCAGGGATTCCGCAGCTCCGCTAGCCAGTAAATAACAATCTTCGGCTTGTCTTGCACCTGATAATTCAGGGTCAGCGGAGTGTCCTTGTATATGATCAGATCCTTCTCGTCGTACCTATGAGAGCATTTTTGAAATTAAGCATGTTTTAAAAGATATATCGTTGTGCACTTTCCCGGCTTCCTCTTTCGTTTCTCGCAGGGCAGTGGTAAAATCATCCTCGCCTGGGTCCACATGTCCCTTGGGAGAACTCCAGTGGAAGGAGCCGTAGGAAG
 

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