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CRIMIC CR01305

Gene Tsp42Ef

CG12845

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:7025488..7025490	42E5	FBti0202716	81216

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AATTGACGTAATCGCTGGAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGACCTAACCGCAGTGAGTG	AGTGGGCTATCTCGGGTGT	CAGCGATTACGTCAATTGTATGC	TTATAAGGGTGGCGCTCGAC

 

Amplicon 5'

Amplicon 3'

TGACCTAACCGCAGTGAGTGTTTTTTCCACTCTTTCATTGGTATTCATAAATTAAAGTATTAAGTATCAAAGTTTAAAGTATTATTCATAAATTTTAATTTTAAATTTGACTATTCACATTATATTTCACATATTATTCTTCCTACGTTTAAAACTGATTGTTTTGAGATTATACGTTTATTTTATGAATAGCGATTGTATTGAATGCTAGTAATGATTATATCTTCATTTTTAGCAAACATTAAACTAGTTTAAAACAGACATTGCCCATATTTTAAAAGCCACTTCTGGGACCCGCCACTCAGCTTTTCTTGTCAGCCTGGCAACGTTGATGTCATTCGGCACGCAAAAGCTACTTCGAAAGCTTCGGAGAAGAGATAGAGATAGCCGGCAGTAGAAGCGCCGTTCAGTGAATTTCTAGCCGACGGACAGACCGCACCTGATCCGAGTCATTCGCCCATGAGTCATTCAACTCGCTGCGGGAAAGTGCCTCGCTGATTCCGACAATTTCCCACCATTGGTGGTGCTACGTTGCACGCTGTTAGATTGAGGAAATTCGACATAGTTGCGACGATCTTCCAGAAAGTATCGATAACGTACAGGGAGCCCAGAGAGAGATAGAGAGATATAGAAACATCGCTGATCTCACTGATCCGATTCGATCTCAAAGAGAGCGGAGGAGTACGAAAACAAGTTTCGCGCAGCTTGCTCTCTCTTCAGGCGGCTCTCCAGGAATTTCAGGCATCTCGACCCCCAACGAATCCGAGGAGCATCATCCGCCATGGCGTCCACGTCGAGTGTGAAGCTGATTGTTTACGCCCTGGACGTACTTTGCACAGTGAGTTACTTTCGCTAGTTTCCCGCCCACACCCGAGATAGCCCACT

CAGCGATTACGTCAATTGTATGCGTTGTAAACAAGAATACGGTACAACGACCACTTAAGTTGGTTTCGCAACGATCCCAAACAATGCACGAAATATATCATCTTATCAAATGGCACAACTTTAAGTGTACCTGCGTACATATATACACGGCATATTTTAGTATGGGAACCGTCGCCATGTGTTAATTTTTGGTTGGCAGGAGTGTAAAACACACCAGTAAAACCGTCTAATGGTCGAACCCAATTCCCCGATTATAGTTTATTGGTTTATTTATACAATTTAATTGCAAGCCGCGGTGAGAAGGACCAGATGGCCCCAGAGGTCACAGCATATATAATACGATCGGGCTATCCGTCCGCTGGCAGCTCATCAATTCGAAATTTTCCAATCGATATCTGGACTTGCAGCAGGGACTCGGCTTACTCATGACTTTCGGACGAATCCATTGCAATCGGAAAACAGTCACACTCGTATCAGAGCTAAATCGAAACAGCTAGCGAATTAATCACCGATGTTTGCACCTCGCCTTTCATTTTCGAATCGAAACATCATCGCTGTATGAGCGCCTCACGTCACCTTTTAAACTTATCCCAATGCTACAGAGACTGAATTTGAATAATTCAAACCCACTCAAACAAGCGCGTTAGCAAACAAGCTATCCGCTGCGATACCGCCGTATCAGATATGAATAGTAATCGAGTGACCTTGTCTGTGTGCGCAGTCCTGTCCGAATTGTTTGCTGTGGGTGTCCTTCTTCGACTTCCCAGTCCCTAGTTTCCCACACCACGTTCCCGCCAACTTCCTTGGCGTCTATCCCTGTCGCCAGATGGGCTAGCCTGGAGGACGCCCCCGAATGGGCTCTCCAACTAACGCCTTGTGCGAGGTCAAAACACTAGAAATGGAACACTAGGCCACAAGTACCAGGACTTTAGTTAAATGGGTTGCTGACGAAGGTAACTATTGCCAATTCAGTGAGTTTCACTCGCAAGGAAAGATAAGCTGAATAACATAACAAACACTATACAATAACAACATGTCTATGAATGTGTATTGCCGGCCAATTTGAATTTGGACGGCTTGATTGTGGGATTTCAGCGGTCTCTTATCAAAGCTATCAAAGCTATCAAAGCTGTCGAGCGCCACCCTTATAA

 

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