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CRIMIC CR01309

Gene CG33199

CG33199

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8950609..8950632	44F11	FBti0206887	83259

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK

Images

Construct Design Sequences

sgRNA sequence	GTGGTGGTCCTTGGTTTGCCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGCCAGCCTATTACGTCCG	ACCACCACAATCGTCGTTGA	ACTCAGAGGTTTCCCCCAGA	TCACACCGTTTTGCCCTTTG

 

Amplicon 5'

Amplicon 3'

ATGCCAGCCTATTACGTCCGTGGTGTCGTAAGTTTCGCTTCTGTGGTGGTTTCCAAATGCTCGATCAAGTGCATTACATCCTGTGTGCGAATGCCCCCCCTGCTGGTGGTTCCCTTGGCCAGTCGCTTAGGGCGTCTCCCTACGCCACGTAGCGCGGGTTTGGCTTCTGTGGAATGGCGTTTAATGGCGTCTAGAAACAAGCCGTTCATTCCCGTGTTTAACTCCGGAACACTGATAATAATATTGACGGCAGGCGGCGTTGACGGTGGTGACTGACCTTGCGGCTGGGTGGTTGCTTTCTTGGGCGGACTGCTGAAGGGAGACCCCTTAGATTTGATGCTAGGAGACATCCTCACACTTGCCTTCACACAAGCGGGTAGTAATGGTATTTCAGCTTTGCCTTGCCAGCTTTGGGACGTAGCTCCCTTCAGATGAGCCACATGTTCCTTGGCTTTGGTGTCCTCAGTTTGTACTGCTTTTGCCTTGGGCGGACTTATTAATCTGTCGGGTAAGACGACAGTTGAAGGTACTCCCAGCTCTGATTTCAATGCTTGTGGGGGTTCCTTGCATACGGTACTGTTCTCCTCCAGAAAGCTTAAGTCGGGAAGCTCCAGTTGTGGGCAGGGTTTTTCCTCTGAAGGCGAGTGCAGATTTGGCATGGAACTGGAACTCACATTTGTCTTAAGGGGGGTTTCTGTGGCAGAAGTCAATTGCTGCAGCAGTTCCAATATAAATTCGTCATCATTTGGAGGAGGTTTGGACGTTAAGGCCGCAGGGTGAGAAGGTAGCGTAGCAGCAGGAGATTCAAAAAGTTCAGATTGTGGAGCAGTGATGGGAAGTGCTGCCTCCGAGTTCAGATCTTCATCAGAGGAGACCTCATCGTTGTGTGGCCACAAACAGCTCCTGGACTCGCACTTCAGCAGCTGCTCCTCGAGATTAATGTAAAAGTAGCGCAGCTTGTGATTCCTGCCTTCACTCTGGCACTCCAGACACTCGTCTCCCGGCTGCAGTCGCTGCATTCTCAACGACGATTGTGGTGGT

ACTCAGAGGTTTCCCCCAGAATGCGCATGCTGTTTCTGCCGTTTGGCTGCCTGGTTTAATGTGCGTGTGCTCACCTGACCGAAGGTTAAACGGAAAGCGTACAGGCCGAGATTGCTTGTTTTCATGGTCCGTTCCAGTTCCAAATCGAGCAAATCGGGACCTGCAACAGTAGTAGACACGGTATCCGGTTATTCGAGCTTACATAACATCAATTGATTTTTCCCCGACTGCGTGCGCTTTGCTCTTGTTCGCTCATTGCTGGCGTTCTTTATCACTTACGTTTCGTTTGGCAATGGGAACTTTAGTGCTCGGCGCTCTCTGATTAACCGCGATTGCAGGTGCAGCCGATTAAACCAGCAACGTTGCTAAAATTTGGCGAGCAAATGAAAACCAAGAAAACTAAACGATCTAGAACTCATGTGAGTAGCGGGCAAGTGCGAAAGGGACTCGAGATTAGAGATGGGACTTCGAGTGGCTGCGATAGTATCGATCTACGGAGCACTTGCCTGCTGCGAGAGATAGCAGGGGTATTTTTTATTTTTATGCTTCATATTGTTAAGAAATCAACGAAAACTTTGAGCGTTAAAAAGAAGTTTCCTTACTTCAAATTCTTGTTGCATTTCCAACGCTTTCAGAATTTGAAACTTTGGAAATGTAACATCTTTAAAGTTGATGGGAGTACACATGCAAAAATATTGCGGGATCATTAAAAATACGAAAAGGTATTTTTCAGACCAAGCACACTTTTTTCTTTTCATGATATGTGTCCAATAGACAACTTGTCGGTGAAATGGTAGTTAAAAACCTTTATAGTGGAAAATATAAATTAAAGAATATCAGATTAAAACCCAAAGGGCAAAACGGTGTGA

 

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