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CRIMIC CR01310

Gene Slik

CG4527

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24400045..24400066	60C8	FBti0206888	83260

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	TCGCGCGGTGCGATTTGGTTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGTCATCGTCCAGGTCGA	GCACCGCGCGATACATTATT	CGGCAGCGTGTAAGAGAGAA	TGCTCATCGAGTACTGCGAC

 

Amplicon 5'

Amplicon 3'

AGAGTCATCGTCCAGGTCGAGCTGGAGCGCCGAGTTGCGGGGTTCCTGAGAAAGATTAAATACAGCTTATTAGCATATTCATACGTAGTGTCGTTCCTAAATAATTCCTGTTCGGCTAGCAGATCTACTTGTCTCTTACTACAATAGCGTTAGCAATCCTACGGCCAAACTATGCTTACATTCGGCATGCCGAATAAGCATAATATTCGATTCTGAAATTCCACCGAGATTCATTTTATTGCCATACCCCAAAACAGCCCTAAAAGAATTTGTTTACATCCGACGTTGAGCAATAAACGCATTGTTCGCAAATTCTATCTGTTATTGCTCCATAAACAATATCTAAGTCTTAACGTAAGGAGCTTATAGATAGTGAAACTGACACTTTTGTCAGCTATGTGCTGGTCAGACTGATTAGTAACTTAAAACCGTGTGTGATTGAAAGTGTGCTGCCTATAATTACCGCAAGAAACCGATAGCGAGGGCTTACGGAGGAAGCCAAGTACAATAACAAACACAACGAGCGAAGTATGCCTCCGACAGAGGAGCTAAGTGATAAGCGGCATTTGGCAATGCTGATAAAATAAGTAAACTAATTGTGGGTCCGCTTTTGAAGGACAGTGCCTGGGATGCAGAAACAGAGGAGGGGAGGGGTGCGGGTGCCAGGAACGGACGGAGCACGGACGGCTTGCTGTAGAACGACTTACCAACCCTCTGATAGAGCGAGCGCCGCTTCACTTGGCGTTTAAGCTTCTATATCAATTATTTCCAAGTAACCACATACAAATGATAAGAACAAACATTGCGACATTTTGGCGGAACAAAACAGAACGTAACATAAACGCAAGGCAAAGAATAGTCGAGAAGAAACGAAGATAAACATATAAAATAATGTATCGCGCGGTGC

CGGCAGCGTGTAAGAGAGAAGAAAGAGCAGTTGGAGAGGTGAGAGAACGTAGAAATTATCATCACATCTAGCAGTTGGACTACAAGGGTGCGGTGCTGGGCACAGGGATCCATGGGCTTCCAAGGCGCACTCACCTCGGTCTCATCATCCACCACCTCTTCCACGACCTCGGCCTTGTACTCAAGCAGCAGATCCTTGATAGGCTTGGCATCCAGATTTCTGTTGATGAAAGCGTGCTGCATCAGCACATCCGTCGTGGGCCTCACCTGCGGATCCTGTTCAGAAGGAATACAAAAGTAAAGTTAGAATGGAATGTAAATAACAGATTTTAGGGTTACCCACTTTGACTAAGGACTTCTTCAGGAAGTCGTTAAACTCCTTGCTCCATCGAGAAGGCTGCTCCAGCTTGGGCGGCTCGCTCTTTTGTATTTTCAAGAGCACTCGCATGGGCGACATCTCGCTGTTGGGCGGCTCCATCTGCGCCAGCTCAATGAGCGTGATGCCCAGTGACCAGATGTCCACTTTGTGATCGTACGGGTTGTCCCGGAAAGTTTCGCAAAGCACCAGCTCCGGGGCCATCCAGTAGGGAGTGCCAATGAAGGTGTCGTGCTTTTGCATGGTGTGCTTGTTTTTGGCCGAAACGCCAAAGTCCGCTGAAATGAGTTGATTTCACTTTGAGATCGTCTCGGCTTAGGAAAAGGGCACCGATAACTTACCCAACTTGACGCCACCTTCCATTGTGAGAAGCACGTTTCCTGCCTTCAAATCACGATGGATGACTTTGTTGCGATGCAGGAAGGTGAGACCCTCGGTCATGTGCTTGCAAACATAGGCAATCTGCGGCTCAGTCAGCGGCTTTTCCAGCTCCACCATAATGCTATCCAGGGCCCCTCCGTCGCAGTACTCGATGAGCA

 

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