• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01312

Gene Tsp42Ej

CG12143

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:7040586..7040612	42E5	FBti0206889	83261

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCATTTCTGGCAGGATCCCATGAAA	CGGCTATAACCAGGCCACTGCAAATT

Images

Construct Design Sequences

sgRNA sequence	TTCCCGAGCCTGACCCAAATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGGAGATTGACACGCTGCAA	GGGAAAGCTCTGGAATGGGG	GAATCTTGCACAACACCCGG	GACCCATAGCTGCCGTAGAC

 

Amplicon 5'

Amplicon 3'

AGGAGATTGACACGCTGCAAAAGTCCCTGAGTGAGGCCAATAATTCAATCATCCAGATCGAGGTCATTGGCGAGGTGGCCTATCCTTGTTATCTGTGCACCAAGAATTTAATCAGAAACGACGCCTTGAATGTGCACATTGGCACCGGCAAGTCTGACATCCGCCACCGAGAAGGAAAGGATCGAGCCACCAATGTGCACCTGATAAACACCATCAAAATGGAGCTGGAGATCAAAGCCGCTAAAGGAGCGCTAGAATGCATAGCAACTCGGTGAATCGTTGCTGGTATTCACAGCATTCTAGGCAATCTTCCACAAAACTGTCACCATCATCATCACTCTCGTAAAAGAAAGAAAAAAGAATTAAACCTTGGTTGTGTTGTTTAATAGCTTCATCGGTGCTAAAAACCTCTGGATACATTAATATTTTCCTAATTGCTTAAGCTCGAATCCATTCAATCATTCAGCAGTTCACATATGTACGCTCATGACAGACCCGATAAGAATTCGAAAACACCACCGTGTGGAAACGCCAGTCGACATGACTTGGAGCTCTGACCCAAGTGCAACGCTCTCTTTTGAGAGAGCGAGTGCGCTCTGTGTGGTTTATTTTGATTGGGGAACAACAGTCCTCACGGGAAGAGAGAAAAACAAAACTTTTCCCGCTTTCAGTTTGCCAGCGAACGCAGAACGCGCCAGACCAAAAAGTTCAGATTCGAGAGGGGCCCTTGGGTCTCTCATTTTTTCGCTCAGTCGCAGGGCAGCTTTTGTGGCATCAACACCACGAGTTCCAATCCGAAATAGGGCTCAGAGCTCCGAGCCCAGTTTAATGTGGGTGTAGACCTACTCTGGGCGCAAGTAAACAATGCCCTGCCCTAAGCCAGTGGCATAGTGCGAATCGAGCGAGTTTCCGGAACTCGGAGGTCATATCTTCCATTCTATCGGCAGCCAGATACTTTGGTTCACAAGCATAACTGCCAAGGAAACTCACGTGCGACATTGTGGCAACATGGAGAAGTCGTTTCCCATAACACCCTGGAAGTACGGCCTACTGGTCACCTGCATTCTCATTGTGGTAAGTGAAAAAGATGCGCATTTCCCCATTCCAGAGCTTTCCC

GAATCTTGCACAACACCCGGTGATGCGAGCAATGAGCCAAAATCGAAAAATCAATATTGCGCCCAAGTTCACATCTTTCGCAAGAGAGCAAAAATTGGTGACAATGAGCCGCTAAAGACTATAAACAAATCGATGCAATCTCAAAAAATATTTATAAATGCATAATACCTGTTCACATACTTACATAATTCCGCCGGCCAGCTGTTTGTAATTTTTGCAATATCTATAAATGGCCCGCAATTCTGGGTGGCTTACGCCAATTAAATTCACTATTATATTATATAATTTATTCTTATACAATTATGCACATGGGACACTGTTTACAAATGTCAGAGATTGATTAAGCATGAAAGCATAAAATGTTCTTGCATTTGCATGCTATAGTCTCATAGATTTTCATTTTGGTGCTTAGCAAGTACAAATGCCAAATGCCTAATGCTCATTTTACCATAGTTTAGAAAACTAGTTGATGGATAGCTGGTAATGTCTGACTGCCAAATGTAAGTTGAAAAAATGACGCTGTAGACGCTAATGAATCGTGCTTGTATTTATTTATTTGGCACCCCAGCACTACACGTGCAATTTATTATTTAAAGTGCTTGTCGCGGCATTAAAATATTATTAGGTCAGTACAAAATGTATATTTAGACTTTGAAGCTGGCTGATCAACGATTTAATAAGTAACTTTCACCTATTAAAAATAATAAACCCTAAAACTATTACGCCTTATCGGTAGGTGTTAAATAATAATGCATTCCATATAGCTAGTTCCTGAAGTTCATACGCACAACCTTGGCAAGTAATTTATTAAGTTATTGGGGCCACACTTTTAGTTTTAGTTTCTAACTCTGCGAGAAAGATGTTCGTTCAAGAAGAAATAGATTAGGAAGTAATTTAGTCCATAATTTTCCTTAGTACTAAGGACTTAACTAACTATTTTGTTTCCCTTTCGTTCAGACGTGCAACGTGTTCTTCTTCTCCTGTGGCGTGACCACCTGGGGTTCGGCCGTCTCCGTCTACGGCAGCTATGGGTC

 

---

Search Again