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CRIMIC CR01313

Gene CalpA

CG7563

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:19426424..19426452	56D5	FBti0206890	83262

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK

Images

Construct Design Sequences

sgRNA sequence	CTTTGGCAGCGCATTATCCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTTTGGGTTTCGCCGTAGA	AATGCCATTACGATGACGGG	GCTGCCAAAGTGTCGTACTG	GAGTTTCTGCCTCCGTGACA

 

Amplicon 5'

Amplicon 3'

TGTTTGGGTTTCGCCGTAGAATTACCAATGTTCGCAAATACAATAACAGATGGGTCAGCCAATCTAGCATATTTCGCCCCAATTAAAGGCCAATTTACTATTGTCACAGAAATATATACCCTATATGTGTCGTACATATGTAGTAGTTCCATGGGTCAATGTCAATATCCCGGCTTAATATGGATAAAAAACGAAGCTTAATCTCCCGAATGGCGCACACCCTTTGAGTCACTAGAGAATCTCATGCTTCCGAATGATGTCACACAATATAGCATATATATATAGCGGCTACATATGTATAAATACAGCATTGTATATGTAGAAACATTTGAATTATATTTGGGTGCGAACCGAGACATTCTTGTCTCCAAAGTATAATTTCAGTTTGCGTGAATTAAAGTAATTTCATTTAGCCCGACTTCCAGTCACACTGGGAATACTAACGATTCTGTAATGCTCGTTAAACCATATTTATGTCTCTGGGTGTGCTTTTAATTCCACCGCGAGGCTTATAAGTAAATATGGGCGATATAAAAAATATTGGATGTTGCTAACTAGTCATCAAATGTTTGAACAATAAATATTTATTAATGGGTTTTTAATCTGAGGCGGCACCTTACACTGCTAACTGATATTAAACTATATATGTACATATATTTTCAGATATCTGCACGATGGACGACTTGAGGGGATTCTTGAGGCAAGCGGGACAGGAGTTCCTCAATGCAGCTGGTGAGGCTGCAATGGGAGCGGCCAAGGATGTGGTCGGATCGGTGATCAACGAGATCTTCATCAAGAAGGAGGCCGACACCAAGAGGGTTCTGCCCAGCATCAAGAATATGAGAGTTGTAAGTTGACCCACACGGTTATCAACAGTTATTTATAGGGCGCTATACTATACTAAACAAACATAGAAAACAATCCATTGAAATCTACATATGCATGTTAACAAATACATATACATTCCACTCATTAGCAACCATTGCAATCTCAAAGTAATAGTACGTTGTACTATGGGAACGTAAGTACCTACTCCCGTCATCGTAATGGCATT

GCTGCCAAAGTGTCGTACTGTGTTGTCCTTGTGTTCTTTTTCTTTCGCATGTAAAGTGTAAAACGCATCATCGTTTATACAACTCCATTACACAACTTTATCGTGTACATACATAATACTCTTAATCAAGTTCATCAATTTTGTTAGATTCATATCACTAATAGCATGTATTTTTTAGCTGGGAGAGAAGAGCTCGAGCCTGGGACCCTATTCCGAGGTGCAGGACTATGAGACAATATTGAACAGCTGCCTGGCCAGTGGTTCCCTTTTCGAAGATCCACTTTTTCCGGCTTCGAACGAGTCTCTTCAGTTTTCCCGGCGTCCAGATCGTCACATCGAATGGCTGAGACCTCATGTAAGTTTTAGATACTACCATATTACCCGTAACTAAAGGTATAAATGCTTTAATTATAAAGGAAATTGCCGAGAATCCCCAGTTTTTTGTGGAGGGTTATTCGCGTTTTGATGTTCAGCAAGGCGAGCTTGGTGACTGGTAAGAACAAAATCTCTATTTCCGCAGCCAGAATAATTAATAGTTTTAAATAAATGCTTACATTTACAGTTGGCTCTTGGCTGCCACAGCCAATTTAACTCAGGAATCCAACCTTTTCTTTCGGGTAATTCCAGCGGAACAGAGTTTCGAGGAGAACTATGCTGGTATCTTCCATTTCCGCTTTTGGCAGTATGGTAAATTATTTATTAAAAAAATTATTCATGCGCTTCTTACAATTTTAGTTTAAAATTTTAAGGTAAATGGGTTGATGTAATCATTGATGACCGTTTGCCCACTTATAATGGAGAGCTGATGTACATGCACTCCACGGAGAAGAACGAGTTCTGGAGCGCCCTGCTGGAGAAGGCTTATGCCAAGTAGGACTACATTATTCACATACTTTATGGTCAACTGATGTATATATGCTTTCTAGACTTCATGGATCCTACGAGGCACTAAAGGGAGGCAGCACTTGCGAGGCCATGGAGGACTTTACTGGCGGCGTATCCGAGTGGTACGATTTAAAAGAAGCACCCGGCAATCTATTCACCATTCTGCAAAAAGCAGCTGAACGCAATTCCATGATGGGATGCTCGATTGAGCCTGATCCAAATGTCACGGAGGCAGAAACTC

 

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