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CRIMIC CR01314

Gene CG12769

CG12769

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:8055467..8055484	44A4	FBti0206891	83263

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band

Images

Construct Design Sequences

sgRNA sequence	GATGGAAGTTCGGTTCATAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTGGCAGGTGAGAATCAGC	CACTTCATCTGCATGCGAAGG	TGAACCGAACTTCCATCTCAT	GTCACGGCATCGATCTGGAT

 

Amplicon 5'

Amplicon 3'

AGTGGCAGGTGAGAATCAGCTTGCGGTTGATCAGCAGGTCTCCATCCTGGCCAGTCGACGTGATGGTCCCGTTGCAGATGGGCGTCGAGCTAGCACCATTGGTGGCACTGGGTGTCGCCGGTGTGGGTGGTCCGCCGCCTCCACCTCCTCCGCTGCCGCCATTCACTCCGGGTGATCCGCGACCGGATCCGTTGCTCAGGCTGTTGGACAGCACGGAATTGTTGTTGTTACTCTCCAGCGGCGTGGCATTCCGGGTGATCCGGTTGTTGGCCTTTGCGCAGATGCGACGCTTGAGATCCTCGCGTAGGAGCGATTTCAGTGGCGAGACCTGGTCCAAAATCAGGTGGATTAATATGGTGGCTATATATTGTATAATTCTTATTAACCCACCTTGATCAGTGAGGTGATTCTGTTGTTGTTGCTGCTGCTGTTGTTATTGTTGGAAGCCTGTTCTGTATCGCTGCTTGGATCACTACCGTTCATCAGGTCCTTCTTGTCCATATCCTCCGACATGGGCTCCTTTTGGGCAATAAGAGAAAAATTTATTTTTTTTTAACACGGAATATAAATGTAGATAATATTTATGATTTTGTTGGTATCTCATTTATTCATTGAACATAATTCTATACTACAACGTAACGATTTTGGGAAAAAAATTAAATTAATATATTATGAACCTGTTAAATCGATTAAAATACTAATATTAAAAAAAAAATAATTCAATCATTTCCATGCACAGAATTTAATACATAATTCATGGCATTTCAAATCATGAAAATTTACTCTAACATTTATTTTACAGGTCAAGATCAAACGCTGAATCAACCATCAAAATAAAACCCCTGGATCTCAACAGTCTATTGGAGCCAGTTCAGCCAAATTAGGTATGCAGTCTTTTATGATGCACAACAGGTTTTCAAAAAATGGTTCCGCCTTCGCATGCAGATGAAGTG

TGAACCGAACTTCCATCTCATAAAGATGAATATTTACAAATTTGAGTTCGATCTGAATTCCTCTTTTCAGTAGGTGGAATTTATGATGTTTGATCTTCAGTTTCGAAGGGAACTACGTAGTTGAGATCCAAATACCTATTCTGGTAATATAAATTCACATACACCCTTCCGTAGCCCCATTTGATAAACCCAATAGATAGATATAATACATAATGCCGATCGCCCGATCTTGCCACTCGTCAACTCACCTGTTTGATAGGTATAAGACTGATCGACGGCGTTATGTGGTTCTCGTGGTGGTGATGGTGGTGGTGATGGTGATGCGGATGCTGGTGATGGCTCTTCAGGCCGCCACTCAGACTGCTGGCTGTGGTGCTGGATGTGATGGGCGTGGCCTTTGTGTTGCAATCCATTGGCAACTCCACCGGGGATTGCATGATGAGGGCTCGATCTTTCACCTTATCGCCATTGGACTCGGCATTTCCATTGCTAATGGAGTTGCAGTTGTTGCTCGCTCCATTTATATTGTTATTGTTGTTGGTAGCGCAGTTGTTGTTATTATTGTTGTTGTTGTTGCCCTGTAATGCAGATTCACGCCAAACGCAAATCAGCTAGTTTATCATTTGTAAGCGAGGGGGCTTGAAAAAGGGATGGGCTGCCATACAGCGATTGACTTACATTAAACAACTCCGTGTTGCCACTCTCGCCCAGCGTCGAGATCTCCACGTGGGGCGTGAGGAAGTGCATGGGGGGCGTGGCATTCTCCAGGGATCGCATGGACTCGCCGCTATCGCTAGCCGGAGGAGCCACCTGTTGCTGCTGCTGCTGCTGGGACTGAGATTGCACGGGCACCGGACTAAGTCCAGGTGGCACGTAACTAGCTGCCGCCGCTGCTGCCGCCGCCGAAGCCTGTGATTCCAGGGCCAGGAGCAACGAAGAGGTGCCACCCGGTCCCGGGTTATCCAGATCGATGCCGTGAC

 

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