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CRIMIC CR01315

Gene MCPH1

CG42572

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:11900732..11900743	48C5	FBti0206892	86507

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CGCAGGATTCAGCGTGAAGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGTAAACAATTGCTGCGCA	GCTGAATCCTGCGGCATG	CTCTGTGGTACGGACAGCTG	AACAGATAGCCGGCAAGAGG

 

Amplicon 5'

Amplicon 3'

GGGTAAACAATTGCTGCGCAACAATTAAGAAAACTTGTTCGCCCTGTCTGTGTACACGCGAATAAATCTCGGGAGTACAATTCCATACCAGCCGGTGACGGGCACGGAAAAGCAGCTCTAACTGTGCAAGATGATTCCAGGCTATGGACCCGTCACGCAGGCTCTGCTGGGCACCCTGCTTACCTGGGGACTGACCGCCGCTGGCGCCGCCCTAGTGATCTTCGTCCGGGGTAACCAGCGGAGGTCTCTGGACGCCGCCCTGGGATTCGCAGCTGGCGTAATGATAGCAGCCTCCTTTTGGTCACTCCTCAAGCCGGCCATCGAAATGGCCGAGAGTTCCCATTTGTACGGAGTATACGCCTTTCTGCCCGTCGCTGGAGGCTTCCTCTTGGGATCGATCTTCGTCTACGGATGCGACAAATTGATGTCATACCTGGGCCTTAACAGCACCAACATGATGATCCAGATGACGCAATCAAAAGCCAAGGCGGACATAGCCATCGAGGACTCTAAGCGCAATGGAGTTGCCCCCGATAGGTTGGCCTCAAAGAGTCTGGACAGCTTCTCCGACTGCCTTAGCGTGCAGCACAGCGGCGAATCGAGACGCCGAAAGAAGGGAGCTAGCATCAACGAGATGGAGCAGTGCACCTACACCACGCCTGAGGAGCAGCAGCGCGCCGAGGACGCCCTGTCCCAGTGGAAGAGGATTATGCTTTTGGTCGTGGCCATCACGGTCCACAACATTCCAGAAGGGCTGGCTGTGGGCGTAAGCTTTGGTGCCATTGGCTCCACGAACTCATCGACATTTGAGAGCGCCCGCAATTTGGCCATTGGCATTGGCATTCAAAACTTCCCCGAGGGATTGGCTGTCAGCTTGCCACTGCATGCCGCAGGATTCAGC

CTCTGTGGTACGGACAGCTGTCCGGAATGGTGGAGCCCATTTTTGGAGTTCTGGGCGCCGTGGCGGTGACCTTTGCCAACCTGATCCTGCCATACGCCTTGTCTTTTGCGGCCGGAGCCATGATATACATTGTGTCGGACGACATTCTGCCGGAGGCTCATGCCAGCGGAAATGGAACGATCGCCACGTGGGGCACTGTATCCGGCTTCCTGATCATGATGTGTCTGGAGGTGGCGCTGTCGTGAGGAACGAACGGCTCGCCTTCGCCCGCCTCGCAGATTTCATAATCTAGTGCTAAACTCAAAGATTCCACTGTACTTAACGCAAAACTCTGCTGTCTTGTAAATAGAAGCATGCTGTGTACAAATCTCAGAACCAGCCCTAAACGAGAATCTAATAATCTAGCGTAGAGTGTAAGTTTTGTAGAAAGAAGCTTGACAACCAGCGATGCCGCGACGACATCCGTGCAATTGTGTATCTGTGCACACGTATCTGTACAGCATTCCCAGCGAGTTATGTAAATGAACTGTTCTAGGAAAATTTGTAAACAAACGTGCATGTGAATTAGGTTTTAAGTTGTCACCGGCGTCCAGTAGCTTGTGTCTTTTTTAACCGTCTAATGTCTAAGTAATAAACTAAACGAATTCAAATGAACATCTCTCAGTTGCACCCACTCGCTTTTTTCCCTCACAAATGTAGCTGTCTCCGTCCGGACAACTTCCTGACCGTTTCTTTGGCCGTACGATAGCTGATAAGGGCAAGTAAATTCACTTAGATAACCCGGAGTGCCAACAACAACAACAGCTGGTTGACTCGCTGTCAGCCAGTGATAAGTGATAAGAATCTGTCCGACACGAAGAGTTCCCAATATTGCGCGTATTCCGTGTTTGAGATACGTGCAGTTTCAGACGGCCCGCGGTCCTGCGTTTCTGTTCGCCATTTTCTGGATTAACTAATCATTTCAGATATGTATGTTTGTACTCGCACAAGTGCTTTGGGCCACAGTCCACCCACTGTGATCGTGGTCATCAGGAAAAGTGGTCGATAAGACCTCTTGCCGGCTATCTGTT

 

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