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CRIMIC CR01317

Gene Osbp

CG6708

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:24936205..24936206	96B10

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CTCTTCCGGATAACCTCCAATGCGAT	TTCTCTCGCTTCTTGCTCGGTTCTCT

Images

Construct Design Sequences

sgRNA sequence	ATGGAAGTAACCCCGATCCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACATTTCGACGCAGGGGTAT	ATCGGGGTTACTTCCATTAC	CCCAGGAGTAACTTGCTTCT	TCCATCTACGGACGCAACTG

 

Amplicon 5'

Amplicon 3'

ACATTTCGACGCAGGGGTATGGATTAAACTTGGTTAGTTTACAGGAATTAATTTATATATATATATATATATAAATATGCTCTAGATCAAAGTGTGGTACTACAATATGCGTATGTGCTGCTCCGAAATCAGTCGGAGCGTGTCCCGCTGTGGTGTGTTAGTTGTGTGTGGAGGCTGGATATCTGAAAACTTTAGAATATTCGCTAAGGCTGCTATGTGTGGTAGTGGCTGCAGTTGCTCCCTTGGCTGCTCCGTTGGCTACTGCTGTCTAGTATATGTCCGGACAGCCTTCGAAGTTCTGAGCCGCCTTGGCCTCCCAGTAAGTGTTCTTGAAAACGTGCACATATTCCTCGCTTCCCTCCTCCTTTTCGCGCTTGAACCACATGGGCTCATAGGCTGGATATGGCCTACCCTCTGCGGCGGCCTCCTCCGCCTCGTTTTCCCGCCGGCGGCGTTCTGTGCGCTGCTTCTCCTCCAGGCGCAATTTCTCCTTATTCGACTCGTCCCAGTCGCCCTGCTCCATCAGCCTTTGGTCGGGACGAAGGCGTGAGTCCGTCGGCGCCACGCCCTCCTCCTCTTCGTTGAGCTGGCAGGCTAGTGTAGTGAAGTTATAAAACTTGTCCGAATCCGGAGGAGCAGGACGACGGCGCCAGGCCAGCTTAAACTCGCCCGTGGTGTACGTTGGACTGCTGACCGATCCCGATGTCTTGAGCACCGGTGCAATCTCGATCTTCATGTCCCACGTACCACGCACCACCCACTTGACCTCGTTGTCTTTGTTCATTACCACGCCTTTGACGCTTCGCTGCACATCGCGACTAAAGTACGAGTAGGGGATAAAGTTCAGGATGCATTTGTGCCCCTCTGCCGCCTGCGATCCCCGGATTTCCATTTCGCCATGCTGGTCCACCCACAGTTTGCCCACAATGATGTTGTTCACCGTTGTGGTCACCTTGCGCCAGGAATAGCGCCTGCCGCTGTTCGGGAATTGCACATAGACGCCGCCCAAGGGATTGATCTGTCGCGAAATTAATGCATTATTAGATTATCACATTGTAATGGAAGTAACCCCGAT

CCCAGGAGTAACTTGCTTCTTTCATAAAGTCCTATACACCTATACTCACTTGAACGTACTTGCCGCGGAACTTGCTGGTCATGGAGAACTCCTGCCAGCACGTCCAGTTCTTACTTTCGCAGTGCAGCGCCGCCACCGGTGGATGATGGGACACTTGCTCGGCCAGGCACCGCCAACCATAGTCATCCATCCGGTCACACTCGTAGGTCTCGCCCAGCAGCGGATTGAAGGGCTTGCCTGTGCGGTTTGTGGTCGTGGCATAGGCGGACACGGTAAAGGCGGCTATGTAGGCCAGCTGCTCGCACTCGTCCTGGCAGGTGGCCGCGTAGTCGAGTATCTCCGTGTACTCATAGTCCTCCACCAGGCGCTGCAGCATGGACAGCGGTTCGTTAAAGTTGATGGGCATGGGAATTTTGGACAGATCCTTGCCAATGCAATTTTTCATGATGCCCCACAGGCTGATGGGGTGGTTCGGTTTATCTGGCACACGGTCGCGTCGCTTCTTAATCAGATCCGGATTCCAGTTGCGTCCGTAGATGGA

 

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