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CRIMIC CR01318

Gene Rubicon

CG12772

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:8677527..8677533	8A1	FBti0210330	86343

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK

Images

Construct Design Sequences

sgRNA sequence	ACATGCCTCGCTCTAAGTGTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCGAGTGTGCTTTTGTGTGG	TGTAGGGTATTCGCCGAGGT	AGCGAGGCATGTTGATTTGA	CTGAATGCGTCTGGGACTGT

 

Amplicon 5'

Amplicon 3'

CCGAGTGTGCTTTTGTGTGGAAAATAAATGCAAATATTTATTTGGCAAAAGTCACAAATTGTGGAACGGATGCGGTGGGCGGAGCTGGAAACAAAATGGTGGCAAAAGTAGTGCGATATTGTTGTTGCTGCTGCTGCAATTTTGTTTGTCTGTTTAATTAAAGCAATATGTGGCCTCCAGGAAAGGAATGTTGGGTTAATTGACTCATCACTTATACGACAAACGAAGCACAAACAAACAAACAAACAAGCCAACGAACACACACATAAAGCAGGAGTAAGAAGAAGAAGAAGAAGAGTGTTTCCCAAATCAGCGCAGTCGACGAAGAAGAAAGAAGATGAAACAGTTCAAAAACCATTTAAAGGGCGTGGCCAGCATTTTGACATTTTGGAAAATTCCACACTGCGCAGCTCCATGACGCTCTCGACCAATCAGAAGTGTGGATTCCAAACGCAAAACTACGCCCACACACATAACGGTAATAGTGGGAACAGCAGGTGTTGTTCACAGAGCGACATCTGCCGGCAGCCCAGCAACACTTCCAAGGCCTATTAGCGGATACAAGAGCCGGCGTAGTCATGACACAAACCGCAGCACGCCTGTCTATGCAAACGAAAAACTGCGAAGATTTGGTATCGATTGGCGATGGGCCTCGGACACATCGATTATTATCGAATCAAACAATAACAAAAAAAAATCGCATTAAAAAACAATCAAAACAAGCTGAAGTTACTCGGAATCAATTGAATCGCGCACCGCGAATATGCAACCGCTCCGCCCCCCCGCGCCCCTTTGACAGTGTGCACAGCCATGACCACGCCCCCGCCACCGCCCACGCTCGCATCAGACGCAATTGAAGGCGCGGTGTACGCAGCAGGCGAGGCACTGCATCATCAACATGACCATCCGCAGCAGCAGCAGCAGCAACAACAACAACAGCAGCTAGCGAGGAATTCCCTGCTGGCGGACCTGCGAAGGTCAACGAATTTCTGGTTCCGTGCGGCCAAATCGACGGACGGACTGCAGTTGCTGGCCCAAAGCTGCACTAGAATTTTGCAGCATGGACTGGTGAGTCGCATTTGCCCGTGGAAATCCCGAAAGCATCGCACACACCTCGGCGAATACCCTACA

AGCGAGGCATGTTGATTTGATCATTCATCTTGCACTATGAAACAATAGTTGAATAATTTAAAGATAAATCATCTTAACTCAGCTCAAGTGGTAAATATTTCTACAGAATCAAGTGAACCTAATACCCCAATAACTAATTATAAAGTAGGGGAGTCTCTAAAGTCTATGCAATCAGCTCTTTGCAGTAAGCAATCAAATATGATATAAACATTTACATATGTATTCAAATGAATTTCCCTGAATGTCTGTCCCACATAAAGAGAGTATCAGCTACTCGCTGCGCGCGTGTCGTAGACTTTCCCCATTGTTTTGCAATCGAATTGTATTTATGCAACGCCTCACTCTCCATTCTTATCAGTTGCAGCCCGTGGAACTTCGCCGACTGGCGGACGACTTTGAGTTTCTGCTGGCCAGTGCGGGAAAACAGCAACAGCAGACCAAACCGCAGCCTTATCGACCGGATTACGCCGCCCGAGACAGTGCGTCAATGGTCAACGGAGTCGAGGAGTTTCTCGGCGAGTGGACTACCCGCTGCCTGCGTGCCCGTTGCCTTTCGCAGTGCCTGCAGACGCTGGTCTCCGATCCGGAGCTAATGGAGCTGTACTATCCCAGCGAGGAAGCCTTCCTGCGACGATCCGGTGATGCCACCGCCTTGTTCGTTTGCCTAACGGCTGTCCAGCTCAATCAGAGCGGACTGCTAAGCCAGCTGGAGGTTAGGCATCAGGTGCGCCACCGACGCACTTGCTCACAGCCGAACTTTAGCATATCGCCCAAACTGCAAGCGGTGCCGGAGGAGAGACTGCAGAAACGCATCTTCCTGCGGCGATTGAAGAGCCTACCAAATTTAAGTCAGGAGGACGAGGAGCTGGACGATAGAGCAGCCTTCAGACGGCGTTGCCAAACATTCAGCAGCTCCAGAAGCCACAAGCTGAGGGAGGATCACTTGGATGCCCCATCTACCAGTTCTTGTTCGTCCCACGACAGTCCCAGACGCATTCAG

 

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