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CRIMIC CR01320

Gene S

CG4385

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1056584..1056597	21E4	FBti0206893	83265

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TCCGAACATAGCCTACTTATCGACGC	TAAGGCCCAGGTGAAACCCACAACTT

Images

Construct Design Sequences

sgRNA sequence	AGTAAGTACCTCATAAGAAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATCCGGGATCTGGAATGGC	TGAGGTACTTACTCCGCCCG	CCTCTAGTCATCGTGAGTCGC	TTAGCCCATTTGTGCGCAAC

 

Amplicon 5'

Amplicon 3'

AATCCGGGATCTGGAATGGCGTGCTCGCCCTGGATCCGCATGTCCTATTGCCATTGCCTAGTAGTTTGGCTCGGTTGGTCTATTGATTTGTGCTTCTTGTCTGGCTTGTAATTACAAGCTCGTACGCCGCCATTCAAAATGGCCTGTTTCCATGTTGATAACGTGAAATGTTCCCATCCATGAACACGTCTTGCATGTGGTAACAGAACAGGCAACGCAAGCGCTGATTTATTTAGCAAATAGGTCCAAGTGGGCCTAATCTATGTGGGTCGGATCTTAATCACCGCCTGCAAAGCGCATTTAGTTGTGGCGAAATGGAAAAGGCCAATGCAGGGCAAACATGTACGTCACGTGATCAGGTGTGAAATGCATTAAGTGGGTGGAAAAACAAGGGAAAAGTGCGATTTAAAAGGTTGAATCGAAAAGAGCACCAACTACTAGCGAATGAAATGAAATATTTTATTAAACATTACCAATTCAGACTGATATATGACAGCGTGGCACTCTGTATGATGATATATGACCCATTGAGACGTGTCAAGAAACCTTTGGCATGGTAAATGACCCCGTTTTAATGCGTTAAACGTTCCCTTTAGCCATTAATCAACCCCGATTGAAATATCTTCCAGCGAAATCCCGATAAAGCAGACACGAGACCGAGACCAGTTGTTCAGCCCCCGCTGTTGTTGTTTTCCTTATTAACCTTATCGCAGTGGGCACTTTTCCAAGGAGAACCCACACGACGCCTAAGAAACGGCGCAATTGCAATGCCACAAGGAGGGTTGCAAAACCCGAGACCCACATGCGGGTGGTGGCTCTGCGGAAAGAAGGCAGGCGGGCGGAGTAAGTACCTCA

CCTCTAGTCATCGTGAGTCGCCGTGGCGCATGCTGTGATAATGCCGTTTGAGCTTTGCTTTTCAAATTGAGCACTCGCTGCGCTTTTCACGGAGCTGATCATCTCCGCCGGAGGCTCGAACGAACCGGGCGAACCACTTGCACCACCGCGACGAAGCGCAGACCATGCCACGCAGTCCAGACGACGACAGCGACGCCTCTGCGGGGGTGGCTTTTTCCCCCCCGGGCTGGGGGCCATGCAACAATGTTGGCAACTGCACACGAAAATTATGAATCACGAGGCAAACTAAGTCGGTGGGAGGAGGGCCCACAATGACATCCGGTGGCTAGCAGGGGAGGTTAATGCTACACACGAAGAAACAAGAGGACAAATTTAGTTAATAGTCGAAGGTTATCAGTCGATAATGTGAGACTTTGTTATAAAAGGGTCGCTGAATAAGGCATATCCAATGTAGCAAAGAAATCTTTTAAAGTTCCAAGATTTTAATTTAGATGTAGGATCAAAGTCTTAATATTCTGCAAAGTACTTAAGACTGGTTAGAAACTGCCAGACTAATAGGGGATTTGTCGTGACCCCTCCCCTTCCACTTTGAATTCCCTACGAACCCGCTGCGTGCCTAACGGGAATTAAGTCATTAGTGGTTGGTGTGTGTGTGCTGTGTGTGTGGTGTGTGTGCAATGGAGTTCAAGTTGTAAATTTCAATTAAAGTTGCAATTTGCAAATTGTCACCAGTGAAGGCCAGACGACAGTGACGACGACATCGTGTAATCACCGCAAGTGTGAAGATAATGCCGTCCTAACATGATTAATGCCCTGACCTTTGGGGGCGTGTTCGCTAGCGTTCGAAGTGCACGGCAAAAAAAACTAGTGTGGGTAGTTAATTATATTGTAATGTGTTGTTACCCAATTCACAATTTTACTTCCTTGTTATCGAACCATAAACATAGTTGCGCACAAATGGGCTAA

 

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