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CRIMIC CR01322

Gene Arr1

CG5711

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:18078857..18078864	36E3	FBti0206894	83266

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ACGCGAGCAAGCAAACCAAGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GACGAGTGCTTAGGGCTCAA	TTGCTTGCTCGCGTAATCGA	AGGCTGGGCGCTTGTAAAT	TATCACCCTCAACGGCGATG

 

Amplicon 5'

Amplicon 3'

GACGAGTGCTTAGGGCTCAATTAAATTAACAACAATTACGTAAATGGAAAAGAACAAAACCCACCACGTAATGTTTGGGTTTAAATACGTCATCCTAGGCGGAAAACAACAAACCAAAATACGCATACTAAATCGATTTCTCGAGCTATTTCTGTACATTCACCTGTCCTGCATTGCTAATACGCCGTGTTGCTCGCGCGTTATTTAATGTTTGAGCCATCGATGTCGATGTCGTGCTGCAATGTCAATATCAAAGACACTGCGCACAGCAAGGTTGCCGAAGCCGTAGTAGTTTACCGCCGTGCAATTGCTGAATTTCTGCTGTGCGGCTAATTGAATTTAGAGGGGCGACAGGTGCCACAATGCCAGGTATAAATGCCGGATTGCCAAAGAGCGCTAATTAATAGCCTAGTGGACCACGCAACGCGGCGTATACCATCGAGAACGAGCGCGAAACGTTAAAGGCACATCCAAAGTTTAAACTATTTCCGCAGAGATTTTGATAAACAGCTCCAAAATGGTGGTCAATTTCAAGGTGTTCAAGAAGTGTTCCCCGAACAACATGATCACGCTCTACATGAACAGGCGTGATTTTGTAGATTCCGTGACTCAGGTGGAACCCATTGGTAGGTGTCACAGACCGAAACCCTTGAGCAATGGGATTTACGAATGAGGAAATCCATCAAAAAATAAATTCGTGTAGGAATTGGTACCCATATTCGATTGAAGTATCTTATAGTTTGAAAATAACTTCAGTGTAACTTTTGTTTCAACTTAACACATTGGAATTTTTAATACCTTCCTTGAAAAGTGATATCAAATCAAAATTATATTATAAAAACTCCATTTCGAATCTGCATATGCCGACCAGCAAATATAAACTCAGGGAGTTATTCAAAATTGCATCTGAATTCAATAGCCCAGGGAGTTAAGTTAAATTGGCTGCGCTAGCAGCTAACCAAGTTCATCGATTACGCGAGCAAGCAA

AGGCTGGGCGCTTGTAAATAATATTCTCCAATTAATAAGCGTCCTCTTGCAGATGGAATCATTGTGCTGGACGATGAGTACGTGCGCCAGAACCGCAAGATCTTCGTGCAGTTGGTCTGCAATTTCCGATATGGGCGCGAGGACGACGAGATGATCGGTCTGCGGTTCCAGAAGGAACTGACCCTGGTCTCGCAGCAGGTGTGCCCACCCCAGAAGCAGGACATCCAGTTGACCAAGATGCAGGAGCGTCTGCTGAAGAAGCTTGGCTCCAATGCCTATCCCTTCGTGATGCAGATGCCACCAAGCTCGCCGGCCTCGGTGGTTCTCCAGCAGAAGGCCAGTGACGAGAGCCAGCCCTGCGGAGTCCAGTACTTCGTAAAGATCTTTACCGGAGACAGCGACTGCGATCGATCGCATCGCAGGAGCACCATTAACCTGGGCATCCGCAAGGTGCAGTACGCACCGACCAAGCAGGGCATCCAGCCCTGCACCGTCGTTCGCAAGGACTTCCTTCTGTCGCCCGGAGAGCTCGAACTGGAGGTCACCCTCGACAAGCAGCTGTACCATCACGGCGAGAAGATCTCGGTGAACATCTGCGTGCGCAACAACTCCAACAAGGTGGTGAAGAAGATCAAGGCCATGGTGCAGCAGGGCGTCGATGTGGTCCTGTTCCAGAACGGTCAGTTCCGCAACACGATCGCCTTCATGGAGACGAGCGAGGGATGTCCCCTGAACCCGGGATCCAGCCTGCAGAAGGTCATGTATCTGGTGCCCACCCTGGTGGCCAATTGCGACCGCGCAGGCATCGCCGTTGAGGGTGATA

 

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