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CRIMIC CR01323

Gene snsl

CG2837

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:4905585..4905607	25B3	FBti0206895	83267

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CATGAGCTCCAACTTGTTGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCATGGCACTGGTGGGATC	TTTGTGTCAAGGTCGTCGGA	CATGTGTGAGAGCCCAGGG	TACTGGAGATACGGTGGGCA

 

Amplicon 5'

Amplicon 3'

TTCATGGCACTGGTGGGATCTGTTGCCTGTGCATTCTGATAATTGCTTGAAGAACTTTCTTTCACTGGTTTCTTGGCCTGATCCTTTGATTTGGGCTTCTGCGGTTTCATGCCATTCGACCAGGCCTGCAGCCAGCTGGCCCCATTCCTCTCCGTGGACGTCATCAATCTCTCCGCCTCCTCCATCACCTGCTGGCGATAAAGTTGACGGGCCAGAGCCTTCTGATCGCTCTGGCTCACCTGGCGCTTGTTGAACGATTGATCATCCTGGTTGGGCTCGCCGATGGGACGCGTCTCCTTCTGGGGACCCAGTTCCACCATCACCTGAAAGTCCGCATGCTCGCCCAGGCGAAAGCCAAAGCCCAAACGGGAATCAGTTCCTGTTTCAATATAAAATCGCATTAAATTAATGGTTCCAATTGTATATACATCATCTTACCCTTTTTAATCGGTGGTATATTGGAGTAAACTATGGATAAGATGGAGGGCAGTTCCCGGGGCACAATAAAAGCTGATGCATTTTGTGCTGTAAAAGTATACAATAGTTAATTGACAGGGCCAGTCCAATGATTTTTATCTCTCTACTGCCGGCCAAGCCCCAGCACTTATGTAAATTTTTGGGACAGTTAATTTCAGGGCGGATTATGTAACCGTTTTATGGTCTTGGATTTTCGGATTCACGGATGACTAACAGTGCGTATGCTTAATATCTTACGGCTCTGAGGTCCGTTTCACTTTGAAGGTCGCTAACAAATTCTGAGTAATACTTTAAGTTGATACTATAATGCTAATAAAATAATTAAGAGTAATCTAAGACTAAGAATTCTCTTTAAACCAAAGCTGTGTTTAAGAGTATTTACTTTATTTACTTTATCCACTTAAAGTGCTTGATCTTATTATTTTATAATGATTTTTTCTCGTACCTAACTAGTTGCCCATTTCAATGGAAAAATACTTAATGATTTTACTCCTTGTACTTTGATCACAAAGAATCATTAGGTAACTCTAATCCGAACAGAAATAAAACCCAAAAAAAAAATCACACGAATCACATTGCGAGACAGACAATGAGACCCATAGACGATCCACAGGTTAAACAACATCTGCGGCTATCCGACGACCTTGACACAAA

CATGTGTGAGAGCCCAGGGTGCCAAAGTCTGCCGTGGAGTCAACTGAAGATCTCGATAGACTGTCGCCGGATCGGTGGAAGTAGTTGAACCGGTTACAAATGCTCCAATCTGTGCGATTCGAACGATTTTAAAGGCATTTACTTACTGTGGAGCAGGCCGATCAGCACGGGCAGCCAGAGGCTAGCGAAGCCCCATTGTTTTGGCCAAGACATCATGGCCAGCAGCAGCAGTCAGCATTTATGTGGAGCCGCAGAAACTGCAATTACACACAGGCGATTACTTCATGCCACCAGCGATATGATTACTATATGTTGCAGAATTATGCCTTTGCCGCAAACTGGCTCGATTTTGGCGCGTTTGCGCAACAGTCAGAAAACCCGATCTCTGACTTGAGGTTGGTCAGGTTGACCGCTTGAAAAAACCAGGTGCGTCATTTGGATAAATATTTGAAATATTTGAGTTGCCAGGTTTTCGTTGGCCGCTTTTGTTTACGCGATTTCGATTCAAATGCGATATGCAAACACAAACACACACACACATACCATTTACTATACTATACTATGTTTTTTTTTTTTTTGGGTCTGTCTCGAAATAAAAGCGTAAATACAAAAATCCACAACGGCAACAACACAGCAACGCGGTTTGGAAATCACTCTCACTTTTGTTCAATGAGCTTCGCACTGTTGTTGGGTTTTTTTATGTTTTTATTTTTAAGGCTACCTTGTATTCGTTTGGTTGGGTTTTTTATTTATTTATTTATATTTGTTTTTCCTTTACAGTTTGGCCTTGTCTCATTTTGGCCCTGATTGATTTCTGACTTCTTTCGGCATTTTTTACTGCTGTAAATTCATTTACACGCTAACCTCCCCAAATTGCCGCTAATAAACTGGGCAAACATCGCGCAGAAATTTCGGATCGCATAAAAATGAAAGTTAGTGGTCATAGAAATGTGTCTTGCTATTTTCGGCAGTACATACTGCAATTTTTCTTTTTTTTTGGTCTCTCGGCAAAAACAAAACGTAACGCACATACGAATGGCATAATTATTAAACTAACGTATTTTCGGTTCAATTCAATTCGATTCGAATTACTTAGCGAGTGCCCACCGTATCTCCAGTA

 

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