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CRIMIC CR01324

Gene GluRIIC

CG4226

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:914725..914760	21E2	FBti0206896	83268

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CCAACAGAGCGTATTCGTCATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAGGAGATGGCTGGAAGGC	ACAAATCTGGGAGATGCCGT	TCATCTGTTTAATGAGCAGACCT	ACGTTATCCGCTGTCCATCC

 

Amplicon 5'

Amplicon 3'

AAAGGAGATGGCTGGAAGGCCTTCGCTGTCGTCTGGTTGCCATGAAAAATTGACACCTCATCGCCTTTCAATGGAGCACCTGGAACCGGGAGCTCCGCTCGTGGGTGAAAGACTGCGGCTGATTGAACCGTGTCCGGCTTTAATGGTCTCTGCTCCACGGGGAGCCAATCGCTGATCCTTCGTCAGCTAAACAAAACTAAATTAAATATTGGAAATATTTGCTTAGAATCTTATATAGTACAGCATGTCAGCTGACAGAGCTTTCCCAGTCAGCTGATCTCCGATCTCCACTTGTCGCATTCGATCTCCAATGGCCCGAAGAGCTTTCCCAGTTGCCATGGAATCGCCAGCCCGTGTGCTCGCATGAAAAAAGAACTGTCCGGAAATAGCTCAGCCCGCTGGCCACAGATGTTCGGTGGGAATTCCAGAGAAGCAGGCATGTGGCAGCGCATACTTTTGCTCGGCTGCATGTGGAGTGCATTTTTCATGTGCCGCAGCCGGGGGCAACAAATCAACATTGGTGAGTTGTGGTTAGGAGTGGGAAAAAGCAGCTACCTACTTTTTTGTGCAACCCGCAGGCGCCTTCTTCTACGACGATGAGCTGGAGCTGGAGAAAGAGTTCATGACGGTGGTCAATGCCATCAATGGACCTGAATCGGAACAGACGATGCGTTTCTATCCGCTCATAAAGCGCCTGAAGCCCGAGGATGGGAGTGTCACAATGCAGGAACATGGTGAGTCCACCCATCCACGCACTCTATTCCAAATAACTCGAACATATTTTCGCTTTCAGCCTGCGATCTCATAGATAATGGTGTCGCGGCTATTTTCGGACCAAGTTCAAAGGCGGCGAGCGGTAAGAGAGTGCGGGAAAATCTTGTGTAGAACGCGAAAGCAAAATAGAACGCGAGGAAAATTGGGCACAAGAAAATCGACGAAAACTTTGACGGCATCTCCCAGATTTGT

TCATCTGTTTAATGAGCAGACCTCTACTCTCTTTTTGCAGACATTGTGGCCTTGGTGTGCAATAGTACCGGCATTCCGCACATAGAGTTCGACATATCGGACGAGGGCATTCAGGCCGAGAAGCCCAACCACCAAATGACCCTTAACCTCTACCCGGCCCAGGCCATATTGTCCAAGGCCTACGCGGATATTGTACAAAACTTTGGGTGGCGCAAGTTCACCATTGTCTACGATGCGGACGATGGTGAGTAGGAGCATTCAAGAGAATTTGTTTATATCCAGGACGAACTTCCCACAGCCAGGGCAGCAGCTCGACTGCAGGATCTTCTGCAGCTGCGAGAGGTTCACAATGATGTGGTGCGGGTGCGAAAGTTCCACAAGGATGACGACTTCCGGGTCATGTGGAAGAGCATACGGGGCGAGCGGCGAGTGGTTTTGGACTGTGAGCCCAACATGCTGGTGGAGCTTCTGAATTCCTCCACTGAGTTCGGTCTGACTGGACAGTACAATGTGAGTAATCCATGGGATCCACTAAGAGAAACAGCTTTTATAAACTTTAAAACAGCATATATTCCTGACGAACCTGGAAACCTACACCGATCATCTGGAGGAACTAGCGGCGGATAACGAAACATTTGCCGTGAACATAACTGCTGCTCGTCTTTTGGTCAATCCCGATCCTCCGCCGGTAAATGAAGCAGACAAAGCCCTATTCCCTTGGGCACACTAACTCATTTTGGTTTCGCAGTACTCCCTACCTTACGGATATGTCACCCAAAGGGACAACATCGTTTACGAAAGCAGCGATCCGCCTCGCACCCTCATCCACGACTTGATACATGATGCACTGCAGCTTTTTGCGCAGTCCTGGCGCAATGCCAGCTTCTTCTACCCGGACAGGATGGTGGTGCCGCGAATAACCTGCGACTTTGCCGCATCCGGAGGTCGCACCTGGGCCATGGGGCGTTATCTGGCACGCCTCATGAAAGGGGTAAGAACTACTTTCCGCATAAACCGAGGAATCGAAACGTTAATCTGCGAGGTGTGTGTTGCAGACTTCCGGCGTGAACAACACGAACTTTCGGACAAGTATCCTGCAGTTCGACGAGGATGGACAGCGGATAACGT

 

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