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CRIMIC CR01329

Gene IA-2

CG31795

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1021698..1021726	21E3	FBti0210331	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	AAATGATGGGTATTGGGGGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CAGTGCAAAAGTCCTGTGCC	GTGGAGATGGGCATCCTGTC	TTGATTGATGTCCACCGCCA	CATCCTGCCAGGCCTTGTAA

 

Amplicon 5'

Amplicon 3'

CAGTGCAAAAGTCCTGTGCCGCTGGGAAAACAGTCAATAAATGTGGCGACACAGGCCGTCTGTCTATTTGTCAATAGGCCAACAGAGACGTGACCTGAATGCCGGACGGAAAAGCAATATTGAAAGCCCCGACGCGAAAATAGAAACTAATTAAATGAGAGATGGTCGGGCAATTTCGAAGCAAGTGAGCGGTCTAATTGCCCTGCCTCGTCTGCGATGAAAACTTGAAGAGACCAATCGACCCAAGGGATGCCACCCACACCTCATAAATATGCGAGCTCATGGTCGCTATCCAGGTGCATTGTTTTTCCGTTGTACCTGGCCCAGCTTTTCCTTTTTCCTCCACTTTCCTCCCCACTGATAAACTGATAAGTTGCGGCGTATGCGCAACGTGGCATGACGTGCCGCATCCATAGGCCATTTCAACGCCATGCAAATGCGTTCTGGCAATAAAAGTAAGAGGCATCCTTCGTACACAGCGGCTAAAAGCTTTTACGGCAACAAAATCCCCGTCGGATTGCAACTATCTGCGCGTCATGCTCTTCGCTGTCCGTCGTGATGGTGATGCAACTATTATTTATGGCTAGTTCGATTTACCACGCTGGCGGGAATGGGGGCGAATGGGAATGGCAGTGAAAAGCCAAGACCTCGACCGCAAGAAGGAAACGGCAAAGCCTGGCCAGCTTTCATCGGGATTGGCAATCAACAAATCAAAATCTAGGACGTCGATGGGGGTGCAATCGTCCTTTTGTGCTGGCCCTGAATTGATTGATAAGTCGGCCACTTGATTGCTGGCTCCCAGTAGGGAAATCTTGGCCAGCCTAAATTGCGCCACAAACCAACGTTTGTTGCTGGGCAAACACAGTGGAGACGCAACAATTTAATAAATTTGCAGTCCCCGGGCGAAATTGAAAATTCCGAGCGCATTACCCTTCTTCTCGCTGCGGTTGCTGTGGCAGCCGCCTCGACAGGATGCCCATCTCCAC

TTGATTGATGTCCACCGCCACACCGCACATAGTTAAGAGGAAGCTCCTTAGGAATTTTCCGCCTGGCCAGGGCTGTTTACTTATGAGACTCGCCTGGCGCCTTGGTGAGTTTTCCAGGTCCTGTTTGCTTTTGGGTCAGGGCATTTAAAAAAGAAAGATTTTCATTTCATAACGACATTTTTCCATTTTTTCTCCCGTCATCTGGCCACGACGCAGGCACACACATACACGCTCACGGCCAGGACACGTGTGCCCCGGACAACGTGTGGTATGTGTAATATGCGGAGGCGAGCGTTGAGAGCTCGGGCGACATCTTTAATGACAATAACGAATGTCCGCACTCTGATTACGTGCGGCATTTACCACAGGCAGCCGAAGCAGCAAAAAAGTGTGCAAACACACATTTCATGGGGCCACTCACAACGGGACCTTCTATAGAATATGGATCAAAATGTATACAAAATCTGAACTTATGGTAAAAGAATAATAAATAGATGCAAATAATCTCAGCATTTGGCTCAAGAATATATGAGCTTTAAAAGGATTTCCTCTTGAATGTAAAATATGTTGCCTTCGAGTTTGAAAAGAAAGATTTCAGCGTTAGAAGGATACATTTCTAAAATCATTCCACATTTTTTTGCTCTATAAACAAACTTTACCTAGGAGAAAAGCTGATTAAAATTGTAATCGCCTAGGATACAGTAAAAGGCGTAGGAACAAGCAGGGAAATAAAGGGTAATGATGTTTCCATTCACATAATACCATTTAATTAACATAAAACGTTTAATATTTTCCTGAGCTTGGGACTTGAGCCTCTTTGCTTTGAATTAAAGTTGAAAGTGAAGTTGTATCAAAGGATTAAATCCTTGCCAAAATGCCAGTTAAAAACATAATTGCTGAGTCTGTGACTGCACGGCACAGTAACCAAAATCGAACTGGTGCTTAAAAGACACAATTACAAGGCCTGGCAGGATG

 

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