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CRIMIC CR01330

Gene Dlip3

CG12767

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:18128861..18128894	55B7	FBti0206898	83270

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGGATGTGTCTTCTGCGCTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTTTGTGTCCAAGGTGCTGC	TTGGCTTTGGGGGTTGATTC	TTCTGCGGCGAAGAAACGTA	TCACTTCTCCGTTGGACAGC

 

Amplicon 5'

Amplicon 3'

CTTTGTGTCCAAGGTGCTGCACAAGGCCACCATCGAGGTGAATGAGGAGGGCACGGAGGCGGCGGCTGCCACCGGCATGATCATGATGACCCGCATGATGACCTTCCCGCTGCAGTTCCAGGCGGACCGGCCCTTCCTCTACGTCATCTGGAATAAGAAGAACATCCTCTTCGCTGGCGCCTTCGTTAAGGCTGCCTAAAAGCCCATTTCTCACTTCTTTCGTATTGTTTGTATTGTTGTTGGAAAAGTATTTTTAAATAAATAAGCGCTCCATGATTAAATTACCCGTGTTTATCGTATTGCCAATTGGAAATTCTTGGCAGATGTAAACAAATTTGAACTAAACACCTGTTTCAATAGAAAAATATGAGAACGTTGTTTGAGGATCAACATTTCGGTTATTTAAATATATGTTTTCATGATATTTGAAGTGCGCGCCACTCATTCAGCCTTGCCACCAAGTTCGTGGTCTGTTATCGATTCGATATCGAAAAGAAGTCGCGAAATGGCCTAAAACTGGCCGAACCGCAAGACCAGCCGAATTGAGTCTGCGCAGAGCGCGTAAAACCAAAACGAAGAGAACGCAGGAGAGTCGAACGGTAAATCGGTCGCAGCAACATAAACTTTTAGAATCGCCAGCGAGAGCCGTCTTAAAAGTGCGAATACAAACAGAAGGAAAAGCGGCCGCCTATGGCAACCTGACTAGCAAAACATTCGAAAGCAATTGGCCAAAGTTAGGACGAAATGAGCCGAAAGAACGGAAACCAGGACACCTTTCCCAAGACGGAGAAGATGCAGCGCTACTACGCGGAGCGCGAGACCACAGGACCCGAGTTCGGTGAGTTTTCCTCGGTTCGCAATCGGTACACAATGGATTCAGAAATGGAATCTGAGTAACCGGGGCTCGCAGAATCAACCCCCAAAGCCAA

TTCTGCGGCGAAGAAACGTAGAAACGGAATTAGAAAACCGAAACAGATTTTAACGATTTTTCCCACAAATCCTTGCTCCCAGACGATCGCCTGATAAAGCTTGTGCGCGCCAATCCGGCCATCTATGATGTCAGCCATCCGCACTATCGCCGTAATCCGGTGCGGGTGGACATATGGGATCGCATTGCCAACGAACTGGGCGCCTCCTGTGAGTATATTGCATTTTATCCACTGCGTAGATTCTGTCGATAAAACCACCCCATTTTTTCTACGCCCACTTCACAGCCCGATTTCTGCAAACCAAATGGAAGAACATACGATACAACTATCTGCAGGAGGTCAAGGCGATTGAGACGGGCCAGGCGAATCCGAACGTACGCAAGCGGCGCTTCACTGAGGATCTATCCTTTCTGCAGAACACGGCCCAAACCTACAATGTGAAGAAATCCCAGAGCTACGTGGCCCAACAGAATGGAATGGGAAGTGACAACGACAGCAATAGCTTTTTGTATCCCGATCCGGAGCACCTAAAGATCGACGCCTCCGAGGGCTACGACATCATCGAGCTGGACAACAGCGACGATGGCTCAAATAGCGACGACAACGAGATTGTGCCCGAGTTGCAGCTGGTCATGGGAGAGTCCAAGCAGCAATTATCCCTGCCGACGACACTGAACGGCACCAGCAGCAGCAATCACAGCCACGAACATGACCAAGCCAGCTCGCCGGCCTCCTCGCCCCTGCTAACGCCGATGGTTGTAATGGGCAATGGCTATGGCCAGGAGGTGCATCTAGAACAGCAGCAGACACAGGAGCAAAAGCCGTTCAAGAATAGCTCGCTGTCCAACGGAGAAGTGA

 

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