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CRIMIC CR01331

Gene CG10543

CG10543

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:21003569..21003587	57B5	FBti0206899	83271

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGGTGATCCTGGAGAACAATAGTCA	TGGACTCAAAGTGAAACTGTCCGTCC

Images

Construct Design Sequences

sgRNA sequence	CTAAGCTGATGCTGTTGCCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAGCGTCGATGAAACCATC	CTCCACCCGAGAACTTGGTG	CAGCTTAGCCAACAATCTAGTGA	GATTCACTCGTCGACACCGA

 

Amplicon 5'

Amplicon 3'

GCAGCGTCGATGAAACCATCAGACCATATTCCCATATCGATGCCCATGGAGAACATGGATTTGAAAGCCGGGCAGGCGGCTCATGGGACTGCCAACGCCATCATGCAGGGCGGTAGTTCGAGCCTCGCGAGCCAGCTACATCAGCAGCAAAAACCCTACAACTCAAGCAATAATCCGCTGTCTATGATGGGTGGAATGATGCAGCAGCAGCAGCAGCAGCATGTTGCTATGCCGCACCACCAGCGCCAGATGATGATGATGCCAGAGGATTTCCCGCAACACGGAGCCTCAATGATGAACAGTCGTCAGATGCCCGCTCTGGCCGCCTTGTCGAATCTGGGCGACACACCGGCTATGAGTGGCCAGCTTAACTCCTCCCTAGAGCTGGACGACAATGACCTGTCGGCGGATGAGGACGACGACGATCTGGACCATGACCTGGACGAATTAGACGCGGCCAAGCAACAACTAATAGATGGCGGCAGCAGTAGCAGCACATCGCTTCAGGCGCCACCATCGCAGCACGGCAGCGGTAGTGGAGGCAGCGGCGGCCAGACGCCCGGTAGCAAAAAGGATAAGCCCAGCTACAACTGCCTGCTCTGCCCCAAGTCGTATCGCAAGCGCAAGTCCTTGCTAGACCACTACAAAATGCATCCGGGCTACTGCCATGACTGCGGTCAGCGCAATGGGAACACGCTGGAGGTAACTAGCCATATCATAAGCCAAATATCGCGGATGAGTACTAAGAATTCACTCTATTAATTTCTAGGAAATAATCCACCACAACCGGACAATGCATGTCAAGGAGTTTCCGTTTGTGTGCGAGACGTGCGGAGAGTCGTACTCACGCAAACAGCAGTTCCACGCCCACGTGGAGTCGCACAATAAGAAGGAAATCAAAAGTGAGTCGACGTGAATGTAAATTTCACCAAGTTCTCGGGTGGAG

CAGCTTAGCCAACAATCTAGTGATTGGTGATCTAACCTCGGTTTCATTTGCAGCCTTTCCCTGCGGCGAGTGCGGTCTTAAGTTTCCGCAAAAGAAACTGCAGCAACACTTCGAGGAGACGGGCCACAAGGCGGACGGCGCTATCTGCGAGGTGTGCGGCGAGGAGTTCCAGTCGAAGAATGCCCTGTACCAGCACATTATTCGTGTGCACAAGCGGGACAACTTCTTCGAGTGCCACATTTGCCATAACCGCTTCACGCTGAAAGCCAACCTGGAGCGGCACGTCCAGCTGCACACCGAGATCAAACGGCCCTACGTGTGCGATCTGTGCGGCTCGTCCTACTTCACATATCCCGCGCTCAAGGAGCACTACAGCAACGCCCACGTGGACGTGTCCGAGTGCAAATGCACGCTTTGCGGCAAGCGCTTCGGTTCGGCCAAGTCGCTGCAGCGACACCTTCCGTCGCACTCGGAGGAAAGGCCGCACTGCTGCAACTATTGCGATCAGGTATGTCTACGCGTAGCCCTTGGCTTGACCAACACAATTTATCGTGATGCCTTTGCAGACCTTCAAGTGGAAAACGCATCTGGTGCGCCACAAGCAGACAATGCACGGAAACGAGCCGCCTCCGCCTAAGAAGGGAAAGCAGCGCTTTCCCAAAACAAGCGAAGAAGGTAAGAATGTGGAGAACTTTGCCTAAAATCAAAAGCCATGCTTAACGTTATCTCCTGTCCAGCAGATATGGGTAGCTTACCGGACATGCCGGGTCCACCGCCTGTGAAGGCCAGCAAGAAGGCAGCAACCAGCAAGGCGAAAGCGCAAGCAGCGGCCGCCGCAGCCGCAGCAGCATCATCCCAGCAACAACAACAGAAGGGTTCTGCAGCAACACCGACGCCGCCACCGCCGGTCTCCACCACACCGGGATGCCTGCAACAGGATCCGTTCAATGCGGCCATGGTTAGCAGCAACAGCTCGCAGTCCTCCACGGCGTCCACGTCGCAACACTCGGTGTCGACGAGTGAATC

 

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