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CRIMIC CR01335

Gene Spn43Ab

CG1865

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:7153185..7153204	42F3		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GTGTGCCTCATCTGGCGGGGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCAAACCCAAGCCAAAGGT	CAAATTGCGACTAATGCCATGT	CCAGATGAGGCACACTTGGA	TCCAGAGTGGTCTCCTGGAG

 

Amplicon 5'

Amplicon 3'

TCCAAACCCAAGCCAAAGGTGACACTAAACTTGGGCAGCAGCACCTCCACCTTTTGCTGGCTCATCGCTCCGATTTCGGCCAGAAGATTCTTACCGGACAGCGACTGCTGCAGGGATCTCAGACCATCCTTGCGATTGGGCAGGAGCAGCAGCATGGAGAAGTCGGGGTTCTGGTAGGGCAGCTCCACCACCTTGGCGTCCAGGGAGTTGACCTCGGCGTAGTTGAAGTTCTGCAGCGTCCACATGGTGTCTACCTTGACAGACTGCCCGCTTCCGGTGCGGAAGGAGCGCTTTTCGGTCTTGTCCAGCCGGAAGGCCTTTTGCCAGGCGGCTGAGTAGGAAACGCCGTTCACGATCACTCCCTCGGTGCGATCATTCAGCAGCTCGGCGCGGAGGATGTCAGTGATCTTGCCGTTGGTCTTGGTGGCCACCGCCCGGTTGATGCCATCCGCCGTGCGCTTGTTGTACGGCGGGTGAAAGTCCAGCTTGTCGATGTTCGAGTCGAACTGGCGCTGGGCCACGTCCCTGAAGGAGCCCTTGAACTCAAGGTTCTCGTTGATGTAAATGTTGTTGGCCAGCCGGAAGTTGTTGTCGCGGGTCAGGGCCTGTTGGTAACTACCGCTGCGCTGGCTCACCGCATCCGCATCGCCAGGACCTAAACGCAGCCCCTGCTGCAACTCCGATGCCGTCTGACCCTTGGCCCCCACGAAGGCCAGCGCCATGGAACTCTGTATGGTCGCTGGCGAGATGACCACATTTTCGTTCAGATGATCGGCGGCCACGGCGTTGTAGAGATCGGCAGCCAGCAAATTGCGATCGGCAGCCGCATCGTAACCCACGGTCTTGGACTGCGACACTGTCGCGAGGAGAACTAAAGGAATCGATGCGAAACATGGCATTAGTCGCAATTTG

CCAGATGAGGCACACTTGGAGAAAATTATAGCTCGAATAATTGTCACATTTAATTCACATATTTTTTCGTGCGATTTGATTCACTTTTTATATAATTTTCACAGAAATAGGAAGGATATTTGTCACTTTGAAATCGATTTGACTTTATCACCAAGTTAGAAACTGTGGCATTACGTCAAATATCAATTGAAAATAGAAGAAATTTATTCTTAATGATAATAAAACACCCAAAATTGTAGCAACTCTTTTGATAGCAACGGCCTTTAAAGTGGAAATACATTCCTGTACAGCTTTCGTACAGTGCACAAACCAGATCGCTTAATTGGGGCTTACGTAATAGGCAGCTGATGATGACAGCCATGGTCGTCACATAAATTGTTGATGGGCGTCTCCTGCTCCGCTTGATTCGCACTTGAGCTCGTCGGAATGTGAACTGAAATCTCTGCGATCGCAGAGCGCCTATAGAGGTTGATCCAAGTCGGATTCAAACACGAAACTCAGCTCTCGGGTCCCAAATACAAATACCGATCAAGTGTGTTTACAAATCATTTGTCACCTTTGGCTGGCCACGGGTGATTCTGTTTATTTCGCGGAGATTTGAGTGGGGCTGATAAGGCGATAGCCCGCCCGGCTTGAACTAGCATTGGAATCGAGAATTAGGTATGCCTAGAAAGACTCAGATGTCGACGATGTGGCCGGACAGCAGCAGAGTTTTGCCATTGCCGATGGCGTAAAAGAACGGGTGATTGATGACCAGGGGCAGGGCTCGTCGGAACAGATCCCCGAAGGCTAGAAGTACACCTGGTTTAGGAGGCTTCTTGGTCAGAGACTGGCGTGATCACTTACAGAACGAGTCGTCGGCATTGCCGCCATCCTCCTGTAGCTCCAGGAGACCACTCTGGA

 

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