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CRIMIC CR01340

Gene CG8046

CG8046

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:9163641..9163660	45B1	FBti0206900	83272

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TAAAATCGAGGTGATCATACGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCCAAACCGTGCTGAAAT	GGAAAGCCCTGAAAACGTTT	TTAAACGACGCTTGCATGCT	GGAGCAGGTACAGCCATACG

 

Amplicon 5'

Amplicon 3'

GTGCCAAACCGTGCTGAAATATTTGGTATGGAACACATTAGAAATTTATCGCACTCGAGGTTCATAGAAACTTACCCCAGCAGAATAATACCGATGCCAAATAAGAAGGCGCTAAACACGTTGAAAATCCCCGGTTCAGTGTTCAGTGTACGGGTGTAGATCGCAACGTACAAGGTGGAAGATATGAGGGGGGAAAGTGCTTGCAAGACACCAAGCAGGGCAAAGATTTTGCCTAGAAAGGGGAAACCACAAATATGTGTGTTAAGAACATGATCATAAGGCATGCCATCCGTACTTACCGCGCTCATCAGCCGGCAGCAAATTGGTGATCATGGTGCGGCACATGGGGTTCACAAGCGACTTGAAAACGCCCAGCCCAATAGCCACGTATATCTGCCAGCTCTCCAAGGCGAAGCCCTTCATCAGGCTGCTGGAGCAGTGGCTCAGCAGGGAAACCAGTGCCAGCCACAGAATCGCCGAATTGGTGCACTGTCAGTCAAAGGGGTCATGGGTCATAGGTCAAGCACCAATTTAGCTAGCTATCACATACAACTCACCTTGCGGAGCGACCAAATAAAGAGAACTCCACCTGAACCAGCCACTGCGGGTACAAGAATGCCAACGGTCTCGTACTCAGTAAACTGCTTTACGGTCCAATGGAACTTTATCCTCATAAACTTATAGAACACAGAGTTGCTGCCGTCTGGCGATAGCCAAAATGAATATATCAAACCAAGATCTTTGTCAATGCTTATCCACAAACAAAAGGACGTGTCAATTAATAACAAACCCATTTCAGAATTTCAATCGATTTATGAAATCTTGAAGTGCCAAAACGATTTAGGTTCATAAACGTTTTCAGGGCTTTCC

TTAAACGACGCTTGCATGCTAAATTATGATTTTTGGAACTTACCCGATACAAAGGCGGTGAGGAGGAGTACTACCATGATGGTAGTGAGCATGAAGCGATTCTGGTGCTCACGAGGCTTCGAGCAGGTGCTCCACAAACTCTTCAGCATGGAAACCACACTGGTGTCAGTCGTCGGTGTTGATAGTGTCCTATTTCTGGCGGGCAAACTCTCCGGTAGTAGGAGTGCCATCAGAAATAGAGCCGTGAAAATGCAAACTGCAGATATGCTGAACACAATGTAGGCGTCCGTGGCCTCGTATGCATAACCAGAACCAAGGCTACCAAGGAGTAGGCCCACATATATGGCCAGTTCATAGGCAATCATCCTAGGGTGCATAAGTCCATAAGTAATAGTAAATCCACTCGATTCCTTGGCTAACTACCTGTAAGATCGAACTTTTCCGCCGGAGACATCGGCAATAAAACAGACGGCAGCCACGGAATAGGTCACACTGCTTCCCAAGCAAGAGAGCGGTACGATGGATAGCACATACCACCAGGGACTGACCAGACCTTGGGTGTACATGGCACAGTAGGCAATCGCAGCTGAAATGAGATACTGAAGGCCGTAGCCTGCATAACAGGTTAAGCACATGGGCATCTTCTGACCAGCGGTAGTTTACACCCACCTAGAAAAGAGCACATGAGAAGGGGCTTGCGTCCATAGTGGTCCGCCCAGGAGCCAGCAAAGAGCCCACAAAAAGCGGGAATAAAGCACTCCACCAATCGCATCGCCAGTGTGACCTGTGCAGCGTATGGCTGTACCTGCTCC

 

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