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CRIMIC CR01342

Gene Bap170

CG3274

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:6638084..6638113	42B2	FBti0206902	83274

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GCTATCATAAATACACTGCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGGAGTTCTGGAGGGACCT	ATGATAGCACCTTGATACTTCGT	CGTAGCATAGCAATCCCGA	GGCTGAGCTCGCATCCTAAT

 

Amplicon 5'

Amplicon 3'

GAGGAGTTCTGGAGGGACCTGCAGCAGTTTCACGAACGACGCGGGTGAGTTGCCGCGGCTCTCCTGTATTTGGAGACGACCTAACCCACTTTCCCTCTGCCCAAACAGCACTCCATTGACACAGCCCGCCAGGATCAGCGGTAAGCATGTCGACCTGTACAAGCTGTACAACGAGGTAACCGAACGAGGCGGCTTTAATAAGGTGACCATGCGGGACGAGTGGGACGAGGTGTACAGCGCCATGGAGACATTGCGGGAGCGCTGCGTTAACGGAACGGCTAGTATCAAGCACATCTACAGACGCTACCTGGACAAGTACGAACGGTTGAACTTCTTTGGCGAGGATCCAGACAAGATGGAGGCTCTGGAGGCGGCTATTGAGATTGTAGAAATGGGCGGTGGACGCTCGCGATCTCGTTTTTTGGCTGGCGCCGGTGGTGGCCTAGGAAGCATTTTTGGATCAGCACACTCTACGTTGCCGGCGGTGCCAATGGCCTACAACCAGCGCCAGCACTCTGTTAATGTGGAACGACGCCGACAGTACAAGATGTCCACGCAGTTGCACCGACACAGCAGCTACGAGAAGCTGCTGTTGTCTCTGCTTTCTCCGCTGCCCAATGAGCAGGACTTCGCCATAAACGTGTGCACACTTATGGCCAACGAGGCGCGCCACACCCTACAGCTGTCCGAGTGCCCCAAACTGCTTGATGTGCTGCTTGCTCACCTGGGAGTCTACGCTGACTTCACCATGCGCCAGCTGTTCCAGCACAGTTACACCGAGGTTCGACACCATTCGCAGCTCAGTTTCTGGCGCGACCTGCTACACGACAAGCCACAGGTCCTAGAGCTCTACACGGATGAGCAGGCCTGGCTGGACAACGGTCTGATAAGTAAGGAGGACGGTGAGAACTCTGCCGCTCGAAGTGAACTGCTGGCGGCCTGCGACGAGTTGCACTTCCTTAATCTGCGCCGGAGCAACGGGACTGATGAGCGCATGGGCCAGCGAGTGCTTCAGATTGTCCAGCTAGTCCGTACACTCAGCTTTCATCAGGAGAATCATGCACTCTTGGCCTCCAACCGCACGTTGTGGCGATATTTGGTCATGGGAGCAAACGTACGATGGAGTAAGTTTTTTTTATCTGCTTAGAAATTATATTTTTTGGCGCTTTGAATGTACGAAGTATCAAGGTGCTATCAT

CGTAGCATAGCAATCCCGAAAGAATCAATTTAAAATAAAATAACTGAAACACGTTTCGCTCGTTCTAGGCAACATTCACATCCAGGCCCTCGAGACCGCTGGCAACCTGGCTCAACAATTTGAGCTGCTAGACCCCACCACTGACGAGCTATCGCGGAATCTTTTAGCTACTCTCTGCGAGGGTATTGACTCTAACGACCGCGCGGTGATCATGAGCTGTCTGGAGATACTCTATAAGTTGTGCGGACGAGAAGGCAATTCGCAGCACATCAATCGTTGCCTTGGCCTGGACTTTTACCAGCGAGCCATGTTGCTCCTCTCGCTTACCGACGTCATGATGATTATCTTTACGCTGGAGGCAGTTTACGCACTAAGTGCTCTTGGCGCTCGGCCATGTTCGATGCTGATGCAAGTACGTGGCCTAATAGACCAGCTGGTGACCCTTATCACAGTGGAGGCTCAGTCATATGGCACAGGCGGTTGCATATTAATGCGTGTCGTAGAGGTTTTCCCCGGAAACATGGCTGCCTCGCTGGCTCAGAACATGCCCGCCATTCTTGCTCCAGGTCCCACTTCCCTGACTACACTTCCAACATCGCTGCAAGTAGCTGTTAAACCGCCAGCAGTTTTACCCACGCCTACACCCGCCCAGACGCAAGTGCTGCCTCACGGAGAGCAAACACCCTTGGTTACAGCTCTCCCCGGTCTGCCAATGCCCAGTCTGCCACAGGTTCAATTGCCTGTACCCAGTCTGCCGCAGGTACAATTGCCACCAATCGCTGCCACCGTGACCCTTCCCAGTCTGCCCTCCGCCTCCCAGAGTTTTACACACGAGGACGAACAGTATGCGCTGGCTTGGCTGGGAGCCACGTATGAACGGGCTGGGAACGGTAACGATCTCCGTGTGGAACAAGCGGAACTATACAGAATATATTTATCGCATTGCCAGAAGGCGGGTAAGCTTTCAGTGGTTAATCACATGCAGTTTCCTCGTCTAGTGCGGCTTATATTTAATCAATCGGTGGGACCAGTAATTGTACGGCACCTGGACGGAATAGAGCTACCTGGGACGTACTACGTAGGCATTAGGATGCGAGCTCAGCC

 

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