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CRIMIC CR01346

Gene CG5080

CG5080

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:1162593..1162610	21F1	FBti0206903	83275

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	AGTGCATTTGTCGGCGCCTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGGCCGACAACTTCTGGAT	TGTGCATCATGGTTGTGGG	GCGCCGACAAATGCACTTAG	ATCGCCGCGAATATGTCCAA

 

Amplicon 5'

Amplicon 3'

CTGGCCGACAACTTCTGGATCTCCTCGAGCAGCTTGGCGTTGAGGCGCTTCTGATCCTCCAGGAACTCCTTGGGAATGGCGGGTGGGGCGGCCGCGGCTGGGGGAGCGATCATGTTGGCGCTGACCGGCGGCCTCTTGAAGCACTCGTCGTTCTCCCGCATCCACTTGAACATGTGCTCCGTTGCCTGGTTGAAGCGGTCCGACTGCGAGTTGTACTTCTCCTCTTGCTGAGGATCGGATAAGTTTGGATGATAACATCTACTACCCCTTTATGGCTGACTTACCGTGATCAGCATGGTCTCTATCTGGCTGACCCGCTCGTCCAGGCGCCCGAAGATCCCATTGATGGGCTCCAGGCTCTTCTGGCCCTTCTGGAGCGACTGCAGCGCCTTCTTGAGCAGCTCGCCCAACGCACGCTCGCGATGCTCGTGCCGCTCCAGCTTATTGTCCAGCTGATTGTAGGAGTAGATCAGCGACTGGATAGTTCCGCGCAGCTCCTCGTTGCTGGAAAGGGTAGGGGGTTAGGGTTAGTGGCTTAAAATTAGCCTCGCTGCGGACCATTAATCATCGGAGTGGGGGAGGGCAGATGGCAGCGGGACCACCACCATGATGGCGCGTCTATAAATATTAAGGGCTGGGATCTCAGGGTCAGGAGCCCTCCACTCCGCACGCTGTTTGTGTGAACTGCATTGATGGCGATGGATGTGGCATGGCTATGTGTGGCTGTGGAGGGGAAACACGGAACACATGCCCATTTGCATGGGCGAGCAGTGGGAGCTCTGGGAGCACTCCCCCATTCCCAGCCAGAGTCCCAAAATAAACAAACAAATAGGCGCAGCGCCAACATCTTGGCTCCCTTTGAAGGCACCACTCACTACTGGGGAGTGGCTGTGCATGGGCTGCATCCATACTCCATACTCCATACTGCACGTCCCACAACCATGATGCACA

GCGCCGACAAATGCACTTAGTTTGAAGTCTGGGCACAGTGGAGGGCATTATGAGCGGGAACCAGAACCACTCCGCGATGTGTGACATGTGTCAAGACCATAAAACCGGAGGGCTGCAGTTGTGGTTCCTCGAATCCACATCGGCCCCTCCAAGAGATTCAAACGAAGAACTTGAAGTGGCAGCGGATCAATTGGGTCTAGAAACTGAAGATCTGAGTGATTTTACGACGCTAATGGAAGTTTCCGATGGAGTTGGGCAATCAATAATGTGTATGGAGAACCAGGAGTGTTGGTATTTAGTGGTTTAAGCGAAGGGTTCCACAATGTCATTGGACCAAAGATCTATGTACTTATTCAAGCTTTTCCCCGAAAATGAATGAAATCTACTCACGAGACCAATCCAGCGGCGATGGGTTGCCATTGGGCGAGCGCAACTAGGACCAGGAGAACTAATTGGAGAGGGCGACTTCCTCCGGCCATCGTGGGTTTGGTGCGTCGATGCGATCTTAAATTGGTAAGCGAGTCCACTGCGTGGGGTGACCTGTGGAAAAAGAAATCCAAATCGAAATCGAACGGAAACGGGGAATTGAGGATGGGTAGCGATGGCGCCTCCGTTCGCCGCCAGTGGATGCGGATACTTCCTCTGGAGCTGCGTCGGCGAATGTTTATCGCTAGCGACAATGGGGTAAACAAATGTAGGCTTATTTATGTGCAATATTTGCGGCGGTGCAGGCGAGCAATAAAGAAATGTCAGTGGAACAAAGCGGATTGGTTCCTTGTCCGGATGGACCTCGCTAAAAATACATACATATGTGGATGCGATATTGTTCTTGTGGCGATAAACAAATGACGCGAATTATCCGCTGGACACTGCATGTGCCAGATTCATTCTCCTCCGCGGGAAGATATGGAGGAAGTGATGCATTCGGGAAATTGGGTCAGAAATAAGCGAGTGAAATTCGCGAGGGCGAATCGAAATTACAATAAGACCAGTTGCCATCGCAATTTCCCCGGAGATTTCTGCTGTAGGTTGGACATATTCGCGGCGAT

 

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