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CRIMIC CR01347

Gene Wwox

CG7221

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:8000511..8000544	28D3	FBti0206904	84884

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGATTCATTTGTGGCCGCTCGAATGG	CCACTCGTCGATAGATAGCTCACTGT

Images

Construct Design Sequences

sgRNA sequence	CAAGTGAACTCACTGGACTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATTCTCGCGACATCCGTACC	CGAGAAATGTTCATGTGGGTC	GGCCGACAATCGCGAAAC	CGCACGAGACTTTTGTTGCA

 

Amplicon 5'

Amplicon 3'

ATTCTCGCGACATCCGTACCAGGAGAAGGAACCACTCAAGCCCTGGCCCAATCAGACCAATCCGTATACGGGCGAGATCGGCGGACCAGCCGGGCCGGAGCCCACACGCTACGGCGACTGGGAGCGCAAGGGACGCGTCTCCGATTTCTAGTGCCCAAATGAAGAGTTCCTTAACTAGTACTTAGACTGTTATTTAAATGCTAGCCATTAAGACTTAAAAACAAATCAAAATGTATACTTGTTTAGTGTAAAAAGAAAATACATGATATTCTTAACGTACAAAAGTTTATTTGCATTGCACTTATTGGGCAGAAACTATTAAAGAATTCAAAATATATCTTTGTAATGAATAGCTGGTTTAAAATGTAAAATTATACCTAATGGAATCATTGGTGGTAGCATAATGCATAAATATATTTAAAAAACAGTAGCCAAATGACTTGGCTAATGCTGCTCCTGCTCCACAAGCTCGGCAATCAGGTTCTCGCTCAGCTTCCACAGTTGCTGCTGCAGTGCAGCACTTTTGGACAACTTGCTGGGCTCGCAGAAGAAGCAATTGTTGAAGTACAGTCCAGACAATCCGGTCAGCTCGTTGGCCGTCGCACAGTAGATACTCGTGGCAGCCGCTTGTTGCTGCCATATGGGGCACAGAAAGCAATTAGTGAAAGTTAAGGGAAAACAGAGAGTTCTTCTCATTGCCAAACTCACCAGCGACTTGGTGAAGGGACGCACAATGGCAAAGAGCAGGCGGTAGAACCAATAGTTTCGTGACAAATCGCTGGACACCATATTGCCCGGATGCAGGCTAAAAACAGAAATGCCTCGCTGCTTCCAACGCTGCAACAAAAAAACAAAAAAAAAGGGTTGGCAAGTGGAATTCAATTGACCCAATTTAAGGACTCACCTGGGCGAGCTCCTGGGCGAACAAGACATTGCATAGCTTGGCATTGTTGTAGGCCATCATGCTCCAGTATTTCTCCGGCGGCGGCGAAAGGTGATGCACTGCCAGATTCTCCACGGGCAGGTTGGCAAATCTGCATGTTAGTTAGTAACTAAGATTATTTGCGACGCATTTCTTTGTCGGTACATGAGACCCACATGAACATTTCTCG

GGCCGACAATCGCGAAACGAGTCTTGATTGCCATTGCCGGCTGGCATAGCCGGCTTTAAGCGCTCTTTATTAATGAGATGCAGAATTAGCGCAGTGCAGGGCGCACAAAGGCTCCCCACGAACAATGGCGTGAGTCAACAAGTGGGGCCGGCGGAACAAAGCCCCACAATCGAACAAGGACCCTCTGATATGCATAGTGAACTCTATGGCGAACTATCTATCTATGTATCTATCTATCTATCTATCTATCTATCTATGTATCTATGTATCTCCAGTTTTCGGCGGAAGCCTCTGGAATCCTCGGCAGTAGCGCATGCGCAAGTCGCACATTTCCTGCGAATCCACGAATGCCGCAACATGAAAACATTTCTATACACAAACACATATCTGCGCGCACATTTCTGGACTTTGGATTTGTTTTCATTTTATTGAATATCTGCGCTCGGCTTGGGGGCGATGTAAATATAACGTCGCAAAATGTATGCAAATTCAGTGTTATTTATAAGTATGGGTATCCATTGGCATCCATGACAAATGAAACCCGTTGGCGTTCCATTTTTTTTCTTTTTTTATATATGTATATTTGTATCTTGTTGTGGTAGGTAAGCAAAAAAGCGTTCTTTTCGACTGTTTGCATGTAAATTAATTAGGTTCTACAGACAGATAAATTGCTGCTGAATGGCATTTAATTTTAGAACAATAACTCGTTGCTTTACCTTAAGACTCTAATTGAATTAATAATTGTTTAATGTCTGGACAACTTTTCCCACGCAAAAATATAATTATTTGAAAAAGAGAAACCTTAAATAAAAATATTTTATCTATGCAACAATGAATAACCCAATTTTTGTGAGCTGATGCAAGAATAACTAATTGTAAGTAATCTTTAATGTTTGAAAATGATAACCAGTCGAACTTCCATACCAACATTCATATCTTTTTCTCGCCCGTGTGAACGTGCAACAAAAGTCTCGTGCG

 

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