• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01348

Gene CG10383

CG10383

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:18694747..18694785	37A1	FBti0210332	86345

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	TGTAAAAGTTGTGAGTCATCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACGTCACCTCCGATTGCAAA	GGCTTACTACGATTTAGGGGGC	AATTCACCGCACACAAGGAA	AACGAATCAATGAACGCGGC

 

Amplicon 5'

Amplicon 3'

ACGTCACCTCCGATTGCAAAAACAACTACCTAATTATCCCATTCTTCGTATTTGAAAACACGCGCCAAGTGCAAAATTACAAAACAAACAATAAGAATAGCACTATTATCACTTCTGATAAGCAGAACTATAAATTCGCGGGGGGCACTAATATTAAAAACTGCGCATTTCAATGGCGCCAAGCAAGTTTTGGTATCTGTTTTTTTTCAGTGTTTCCTTTTTTTGTGGGGGCCTTGAACAGCTGCGAACGCACACGCTGAAGAAGAAGAAGTAGAGGCGAGCCAAAAACAATAACAAACTTCACCAGCTTGCTATCTCACTTTTACAAAATCGTATGTTATGTTTTGATCGTGTTTCGTCGAGAAAAAAAAGAGTCTGTATAATGAACAGCATGATTATGACCACGAAAATACCCTTTGAAACTTAAAAATACTCTTTATGAAAATAGGTTTTGTCAGCTCTAACTGGTCTTGAATCTAGTAAAATGTCTGATAAATGATGGTTATTTGCTATAGTGAAATGAGATCAATTTATTACTGTAATTTTAAGATCAAGTGCAATTCTAAATCAAAATCCTATCAACATATTATCAACATCCATTTTAAGTCACCCCACCGAAAACCCTTTTGCTTGAAACCAATTTTCCGCTAATGCCTGATTATTAACCGCGCCATTTGTTGTAAACAAATGACGTCACAGCTGCGACAACACCCATTTAGTATAGGGAATGCGGAAAACAAGATCTGTTTTTGTTGTTGTTTACTCGATATATGCATATAGTATGCACAGATCGTACGACTGATCGGTGCAATGTGGCGCCATGTAAAATGGGGCAAAAATAAAATGAAAACGTGTGCAGACGAGCCCCCTAAATCGTAGTAAGCC

AATTCACCGCACACAAGGAAAATTTTTTATACGAAATTATAGTCTTTTTAGTAGGTAAATTTTATAAGATTTGTACATTGATTAGTTAAAAACACTAGTCCATACTTTGCTCGCGAATTATTACATTTTTAAATCAAAAATGGAAGAGCATGTAATAAAAGGGAGCTACTTTTTATTTTCTGTGTACTGCGACCTGTAGCAGCTGCTTGGGAAATGGGTTTGTTGTGGTTTTCTCGGTTGACATGGCGTGAATTACTTACGCAGTAATAAGGCCAACTCCTCCAAGGCCCAATAACTTCGGATGACGGCGCAGCTTGATCTTGAGATCCTCCAATGACATTGCCTGCTTTCTGATGGTTTTGTTTCAAGTGTTTCTTTGTTTTTCAGTGTAGCACTAGGCACACACACTAACGGGATTCACTCTGCAGTCGACTGCCAAAAGTTTGCTCAGTTTTTCACACTTTAAATTTAGACGATTGTATCACACACACTGGCCCATGCAAATGATAGCACTAGGGACTGTTTAGACTTTGTAGCTGTCACTGGGACTGCTTATAGTTTGTTTTGTTGCTTGCTTTTGCTTGTCTGTTGAGCTCTTATTGCTGGCGAATTTATCAGGACTTTCGTTTTTATAAACTAGCCGTTGGTGTGACCGTGCGCGCCTGTCAGCTGTTGCCATTCGCAGCGAGACCATTCAGCTGACGGAAAACAGATGTGGGTGGTGTAAAGCCCATGGGCAGGTGTTGGTGACGTTTCTGGGGGGTCGAGTTAGAACGAATTATTTTCACTAGAATATTTTCATTTTTCTAAACTGCTTAAATAAACTGTCCATTTCACGCAACGCTGACAAACTGTAATTTTGCAGCGTTTAAATCAAATATTTAGTATGACCGAATCCGGCTTGATGCAAATAATTCACCACCTCCAGCATTCACCGCGAAACGCACCCACTGCACAGCTGACTGATAACACAATCGGAAGGGGAGAACTTCATTTTGGGGTATATTTATTTTGGGCCACAATTAGTTGCTAGCATTTGATTGAAGTAGCTCCAGCTCCATTGGCTTGTTTGTAAAATGCCCTAGAGTTCGCTTTCCAACGCCGCGTTCATTGATTCGTT

 

---

Search Again