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CRIMIC CR01349

Gene CG9331

CG9331

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:20826316..20826319	38F1	FBti0206905	83277

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAGTGCAAGCGACAGACGAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCAAACCAGTTGTGCGTCTT	GCGGACAGGCGGTATTCTT	GTCTGTCGCTTGCACTTTTG	GAGGTCAACAAGGACGCAGA

 

Amplicon 5'

Amplicon 3'

GCAAACCAGTTGTGCGTCTTATACATACGCCGTAGGAGAGACTGAGACTCACATCAGCTGCTCGGCGGCTTGAACGCCTACAGACATACATACATACGTACATACATATATAGGGAGAGATTTAGAGCGCGGATTATAATGCTAAACAGGTTCATTGGTTTATCGGCTGCGATCGTGGGTCGGAGCAGTCGGATCAGCGGCTTTCCGTTCTCGCCGCTCCTTAAAGCTGGGTGCGTATGCCGCTTGAGAGTTCAGTCGCTCGCGAAACACGACTTCGATCGCAATACTTCGAGATGAGATCGATTGCCACCAACTCCCTGCTGCCACTGATTGTGGCCTTGCTGATCGCCGGTAGTAATGCCTCCCTCCCACGCAGGGGCAGCAGAAATCAAAGCCAGAATCGCACCATGTCCGCTGGCAAGGCTTTCAAGGTCCTGGTGACACATCCCGAGGTTCCCCAGGAGGGCATTGATCTGCTGAAGGAGAACTGCGAGATCGTCCAGGTGCAGAGTGTTCCCATCAACCGGGCGGAGCTGCTGGAGAAAATCCGTGGCGTGGATGGCGTTCTCTGGGGTGGACACGAACCCCTCAATGCCGAGGCCCTCGATGCTGCCGGTCCCCAGCTGAAATCCATCTCCACCATGTCGGCGGGAATCGACTACGTCGATGTGCCGGAGGTAAAGAGACGTAAGATCCCGCTGGGACACACGCCCACCGTTCTCAACACCGCCGTGGCCGATTTGGCAGTGGGTCTCCTGATCGCCGCCAGTCGCCGTTTTCACGAGGGTCGCAAGACCATCGACAAGTGAGTACCACTAGTTGGTTAGTTGTGTTTGTGATGATTCATATACTTACAGAATTAATTTGAGCAATGACAAAAATAGAGTAAACCGAAATTATAATTGTCATAATTATTATTATAATTAAGCTAAACTAGGGACACTTTATTTTCAACTTAGTGTGGCCGTGAAATGTATACGCATGAATTCTCGATTAAAATTGCAAGCCGGTTCTTAAGAATACCGCCTGTCCGC

GTCTGTCGCTTGCACTTTTGCTCTATAGCGCTCACAAGTGGACAAAGCATTATCATCAATAGGTTTTGGCTCCTCACATGCATAATGTAAAATTCTAAAGTATTGACTTTCGATTTATCTACAAACAAAAGGTACGAGCAGTTTATGTAACCGCCGCTAGGTGGCGGTGCAATAAAAAAAAGTTTTTTTTATCTTCATCTTAGCTTAGTAACAGGCATTTATCAGTATCTCGAGTATCTACAAGTATATTATATATTTTGGAAATATTCATATATCTTTCAATTTCTTAATTTGTATCTTTTGCGTTCTAAAAGTGACAAGTGGGAAAACTACCATCTTAACTGGCTGTTGGGCCAGGATATTCGTGATTCCACCGTGGGATTCTACGGCTTTGGAGGCATTGGTCAGGCTATTGCCAAGCGTTTGTCTGGATTCGACATTGACAAGGTGCTGTACACCACCCGTCGTCGTGTTCACAAGGAGATCGAGGAGGAGTTCAATGCCAAGAAAGTTGACTTCGATACCCTTCTGGCCGAGAGTGACTTCGTAGTGATCGCTTCTCCTTTGACGAAGGACACACAGGGCGTCTTTAATGCCACCGCCTTCAACAAGATGAAGCAGACTGCTGTGCTGGTTAACATTGCCAGGGGCAGTAAGTTACCAGTATTAATTGGGATTCTCGACCGATTTTAACTATTTATTTTATTCCTCAGAAATCGTCAACCAGGATGACCTCTACGAGGCTTTGAAAGCCAACCGAATCTTTTCCGCCGGACTCGACGTCACGGATCCGGAACCATTGTCGCCCAAGGACAAGCTGCTGACGCTTGACAATGTTGGTGAGTGGGAAAATGAAAATAATGTTGTAGTTTATTGATATCAGTCCTCAGGCCCACACCTAGCAAATACTCGTATTTCTGGCCCCTTATCAATATAAAGATTTTGACTTATCTCTTCAGTGGTTGACAATGACTAAAAGTATTTTGAAATTAATGGAATCTTTAAGGTTTCTTGAAGGTTACAAATAAATAGGGTAACGTTTATTCTTTCCATGTTTTATCTAATTTCTGCGTCCTTGTTGACCTC

 

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