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CRIMIC CR01350

Gene Myo31DF

CG7438

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:10498984..10499014	31F3	FBti0206906	83278

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GGTTAATTTACACTCGCTGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGCTCCCGACCCTTGTAT	ACTTTCGGAACACTCTGGGT	AACCCAGATGAGCTACATAACC	CGAGTGTGCTGTTCGTCATG

 

Amplicon 5'

Amplicon 3'

AGAGCTCCCGACCCTTGTATTTGCGAATGGTTTCCGGACCGTAGATGTTCATCTGTCTGTAGGGATTCATCGAGACGCACACTTCGCCGATGTATGTGTAGATGGATCCGTTTTGGAATCTAGAGAAAGAGTAATGGACAGTCGTTCATTAGTTCGGCAGTGTTCGCTGCAGTGTTTGGAATGGCATTTCCCCAAGCTTACGAAATACATTTCGAGTGAGTGGGAGTGACAGGTGGGGAGCCACCACCCGTCGTTGGCGCCTGTAGCTTTCAATTCGAAAATTGCTTTTTCAACATTCTACATGCGGGTCTATATACCTGATATACCTGCTCCACATGAAGCCAACGATCGATATAATTGAGCGAGATGCGCTATGTAGATCAAATTGTTGATCTCTTTTCTCACTTTTTGATTGACTGACATGTGGATGCATTTATTCTGCACTCGATTTGTCAGGCGCCAAAAGATTGGGCATATAAAAGTTTGTTGTGTTCATAAAATGCATTCGCTAAGTGGGTCACTTGCGATCGATAGACAATGAGAAAGAAAGAGAGCATGAGAATCTCAAATATTTCACTTGGAGACTACAATATAAAGTATATATTATTCTGAACTAAAACTTTAGTTGATAAGAGTTCTAATAAGTCTTTTTCATGACACAGGCTATCTTATCTGAAGCGATTGCGAGTTCTTGAAACTATCGACTGGATTATTATAAGTTGGAAACTAAGATTTTGCAAAAGATACTTTTTTATTGAACTTTGCCTCTTCACAAGTATTTAGATTAAAAGTGAGTTGAATTTACACATGATAGAGTGATTTTCTAGTTTGCCTTAAGAACAGTTTAGATTTCACTGCATGTGAGTGGAATTGAAAAACAATAAAGCAACTTAAATGCAAATTACGCATTACGTGTTCCCAGAAAATTACCCAGAGTGTTCCGAAAGT

AACCCAGATGAGCTACATAACCAAAAGTCCAGCCCCAAAAAGAAAAAAAAAACAAAACTGTGCAACAAGTGGCTATTACAAAAGGCTGGACAGAGTGTTATTTTCCCTCCCAAAACCAGAAAAACAAAAAGCCAAAAATGTAAATGAAAATGAAGCCAACAAATTAGCAGACAAAGAAACGGTAAAATTGTTTAAAGCCAGAGACCAATTCGAAAACTAAGCCCAAACCAAACAGCCAAAAGTTTTCGTTAGCCCGAATTGAGGGAATGAAAACGATTAAAACAATACACTTAATACGCGCATTTGTTTGTATTTATGTTTTGGCTGCTGGTTTGGTGCCCATTTTTTCTTCGAACACATGTTTTTACTTAACTTTTCAGTGCTTATTTATAGAGACGACTGCGCTGGCTTTTCAGCTTTCTGTTTCGTTTTGTATGCCTTTGGCTGCCCCGGCACTTCTATTTGCCTTCTGTTGCCCTTTTCAACACATTAAGAATTTTGCGTCAAAACGAGTGCGACGAGTGACAAATGGCCACCTGAACTCCGGCTCCAGAATGGCCAAGAAAGTCCAAGACGCCGTTTAGCGCGCTTTTCAACTTCTTTTTTGGGGCCATCACATTATGGTAAGTACATACATATAAGTTGAACTCAGTGGCAAAAGGGCAGGCAGCAAATGACAAGCCTCTTAGAGGCAAAGGAAACCCAATACGCCACAGATTTGTATGATTTCTACCCCATCCTCCCTTTTACAACAACCATATTGACACCATGTGCGCGCCACTGCGAAGGGTCTGTCCTTGTGTGTGTGTGTGTGTGTGGGTGGGTGGTGATCGCCCACGGGGCGTATGATTGACATCATGTCAAAATTTGTTTTTGGCCCGCATGACGAACAGCACACTCG

 

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