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CRIMIC CR01353

Gene Gr43a

CG1712

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:7392762..7392787	43B2	FBti0206908	83280

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK (weak)	OK

Images

Construct Design Sequences

sgRNA sequence	CTACGGGCTTGTCCCATTCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGGTGCTGACCTTTTGTG	CACTCGGAGTGGAGTCCTG	GGACAAGCCCGTAGAGATTAGT	ACACTCCGTCTAGTCTGGCT

 

Amplicon 5'

Amplicon 3'

CACGGTGCTGACCTTTTGTGGCTTGATGGCACTGGTTGCATTGTTCTCTGTTGGGTGGTTTACAACTAATCAAAAATCCTGGAAATTTAAATTACCACTTACCTGCAAGAAAGCTGCTCGAAAGCATTCGGTTCAGACGGCCAAAGCGCCTTCCTAGTCCATAGGCCGTGTTGGCAATGTTAACCTGCAAGCCCGTGAGGATGAAGTAAAGACTATAGAATGGAATGTACCAGTGCACATTGAGCTCTGTCGAAGAGAAAATGAAAAGGTGGGGATTCAAAGATAGATTTCTTTCATTTTAAGATTTATTTGGCGGCTTATAAATGAATACATTAAAGCGCTAGCTACCCGTATCCGACTGAGCTATGTTCATATCCTGGGCAATGCGCATCCACGTCCCTACATCCAGGCCGACCAGAATCGAAATGGCCAGCAGAGAGACGGCAGCCATGCCCAAGGCTCGCCATCGATCTCTTCGAAAGTTATTGTAGGCATTTAGGGTATTATCGATCTGGGGTGTTTATTGGGTGATTATAACAAATAAAAGGAGGGTGGATCTATGATTGACGTGATCTCACCTTGTTTAGTCGATCGTTGAGCTCGAGAGTGTCGTTCAGGCTGAAAAGGCCACAGTAAACACCGGATACAATGGCCATGACCACCACAGACACGTCCAGGGCCGTCACCACCTTCGACGTAGCCGACTTCATTCTAAAAGATGCACTTTTGATTTGTTGGTTGGTTTTGTTTGCAGTTTGTCAGAGCAATTAATTGGTCAAGATCGAATGTGTTTAGAAGGGAGCAGAGGTGATGATTAGATAGAGAAAAATCGAGAAAAGTATACACACAATTAGGATCAAAGTAATGACACCCTCCGCAACAAAATCATATCCTTCTTGAGAGGAAAGTATTACTTATACATATTTGATAAGATATGGGTTCTAAAAGTGCAGGACTCCACTCCGAGTG

GGACAAGCCCGTAGAGATTAGTAAAAAAAAAACGGCACAGAACTGGGACCTTACAAGTAGGAAACATAGATGTAGATACAAAAGTACCTACCGCACGGGAATTTCGGAGTTAGCATCGAACAGGAGACCCCGATAAGTGAGAAAAACTACGCAGATGCGGATAGCGTTTAGTCAGAAGGAAGTCACCCGCCTTTGGATGCTCAACTCACCCATAACCACCGTTAATGTGGCCGAGTATACAGTGAAGAGCCAGCTGCGACCAATTTCCACCCGGCCCTTGGAGTTCCGGACCGTAGCATATGGAGCCAGGGCCAGCACTTTGCTGATGTAGAAGATGCCGATGCTGGGCTGGGATATCTCCATAGTAATTCAGAACGCGGGACACTCGCAGTACGACCTCTGGACGAGAACTGTTCTCGAGTCGCCAAATACAGGACCTTAAATTGCAAAACGCCATATTCGACACCAAACAAATACTGCGCTCCCATATTTGACTATTATTAGTGGCCACCACCATCCACGCCCCACACCATGACCACATGGCCCTCGCAGACCGTCTGGGCAAACACCCCATGTGCGAGTGAGTAACACATTTGCTTATCAAAAAGCTCGGCTGTCCGCGATGTAAATAATAATTAGCGTGTGAAAATACCAAGCCGGAAAAAGGACACAGGAGCAGGAGCCAATTATTTTGGGGCCGACGTTGACAGCTGCGGCAGCTGCGACAGGTGCACCCGAGTGAATGAGGTCCACAGCCCAATTGTTACACAATTTCTCATTTATTGTTGCGACTCCACTCGGCGGTCCAGCCTAGTGGGGATCGCTATTAAGGAACAGTGGCAGATGCGCAATTAATCAAGTTAATTCTATCTGCCAGCCAGACTAGACGGAGTGT

 

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