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CRIMIC CR01354

Gene ppk9

CG34369

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:21964952..21964959	58A4	FBti0206909	83281

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	CTGCGGTCTCTGCTACTTTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAGCCACACGTTTAAAGCC	TGGCCGTAATGAGGTTTAGCT	AGCAGAGACCGCAGCTTAAC	TTAGCCCCTCGGATCTCACT

 

Amplicon 5'

Amplicon 3'

CGAGCCACACGTTTAAAGCCTGCACTGCAGTGTAGTATTAAGCAGGGGACATCGACAGGCGATTACTGTAGCGTTTTCCCCGATGTCGGGTTTTGGCAGCACCGCGTGGGAAAATCGAGTTTATGTTATATAGGTTTCTATGTGGGAAGCATAATATGAGTAGCTACTTTTTAAAGAAAACTTATTACAACTTTTTATCTATTATACACTTCAACCCATTTACTTTCAAAACCTGCAGCAATAAATCCCATCTTGCAGAAGCGCCCAAGGATATGCAACCGCGAATCGACGACAGCTCACCGTCAGCGGCGGCAAGGACACTGACATCCTCGTCGAGCAGGAGCAGCTGGGCGGAGAACGAAGGATCCGCATCCGGCAGCGACTCGCTGCTGACGTCGTCGACAGCTTCGCTGCTGCCGCCGCAGGATAATAATAGGAGGTTGGGTCCAGGTCCTGGATTGCTGATTTTTCCCGGCACCGGTCAAAGACATCAGCATAATCCAAATGGCAGCACGGGCAGCAAAACAATTGCAAAAGCTAATGACGGGAACGCGGCCATTAATCAGCCAGGACCCGCCAGGAGCATTCGCTCCTTTCGTCGTCCTCCACTGTGGCAGCCACCGCCACAGGATCACGCGCAGGATGGGCAGGAAATGGAGGTCCTGGATCGGCAAAAGCGAAAGGATTGCCGTCCTGCCTGTTTGGTTAAAGGGCAGGCCTTCATAATCGAGTTCCTGCAGGGCTCCTCCATACACGGATTCATTTATTTGGCCAAGCTCGGCCTGAGTTTCGTCGAACGGTGAGTTTTGATTTGTAATCCGCCAATGGATTCCATTGGGTAATGGAATTGGACACACAATGGAGACTCTATTCCCATCGGCTTTTAAATGAAGAACAGAGCGGACTGAAACTCATATTGTAAATATTACTAGAACTAAAGTAGGAATATTATGTATTCCTTAACCCGCATGCATTGGACAAATATTTGGTGTGAGAATATTTCCACTCAATTAATTTACAAGGGAAATTAAAGCTAAACCTCATTACGGCCA

AGCAGAGACCGCAGCTTAACTTTGGCACAGGATAACCAAAGTTCGCCGCTTAGAAACCGTAAAGTGCCAACTAAAACGATACCAAACTATAAGTCGGGGTCTTAAAAAGGACTATCCAGCGAGCGTGGCCACGTGCAGAGATTCCGGATCGGATTCATAACGTGGCTGTTTCAAAGGGGGAAGTGCGTCGTGCCGAGCCCCCACATGAAAGTTCTCCAAGCGAAATACATTTTTCTACATATCTTCCGTTTTATGCGGTCGATGTGGGAAGGTGGATCTTGTGGTCTGAAGTAATATTTCTGCTGCTGGAATTTGTATTTTTGTGCGGTGCAATAAATCCTTAAAACTGACATGACATGACAAACAGGATTACACACACACTAGCTGTGAGGGTCGTGGGGGGGGGGGGAAATTCTCCGCCGCTTTCGCTACCTTTCCCTTTTCCGTTTGGCAACACTCCACCAAAGTAGTCGAGTGGATGGGGCTCAGGATGCGGATGCGGTGGTTCGTGGGAAATCTGTCAGGACTGCCGGCTGAGGGTTAATTAGTGGATAACAGCCAGGCCAAGGACAGGGCCAAACAGGCACTGAGAGAATAACCTATATACTATTTTAATTATTAAATATTTTATTTTCAATATACAATTATGTGAAAAAACTTTATCGACTAGGTACGTTTAGTTGAGTTTATATTCATTTCATGACTATTAACATATTTTTATTAAATAACACATCTTCGTATTCTTTGAGTGCATTACAGTCTCCCCTAAAATCATGAAGCTCAATTACAATTATTGTTAGTTTCCTCTACAGCCAAAGTCCAACTGACAGGTGTTTCCCAGAATAGAAGTGAGATCCGAGGGGCTAA

 

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